Identification of bioactive peptides from brewers’ spent grain and contribution of Leu/Ile to bioactive potency
Brewer's spent grain (BSG) is a co-product of the brewing industry and is a potentially rich source of bioactive peptides. A BSG protein hydrolysate, manufactured at a semi-pilot scale, mediated significant hypotensive effects 6 h after ingestion in spontaneously hypertensive rats. This hydroly...
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ftdoajarticles:oai:doaj.org/article:71838990c9e643e28c652311fda61fe0 2023-05-15T16:55:50+02:00 Identification of bioactive peptides from brewers’ spent grain and contribution of Leu/Ile to bioactive potency Maria Cermeño Alan Connolly Martina B. O'Keeffe Cal Flynn Adeola M. Alashi Rotimi E. Aluko Richard J. FitzGerald 2019-09-01T00:00:00Z https://doi.org/10.1016/j.jff.2019.103455 https://doaj.org/article/71838990c9e643e28c652311fda61fe0 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S1756464619303792 https://doaj.org/toc/1756-4646 1756-4646 doi:10.1016/j.jff.2019.103455 https://doaj.org/article/71838990c9e643e28c652311fda61fe0 Journal of Functional Foods, Vol 60, Iss , Pp - (2019) Brewers spent grain Hypotensive Antioxidant Peptide ACE DPP-IV Nutrition. Foods and food supply TX341-641 article 2019 ftdoajarticles https://doi.org/10.1016/j.jff.2019.103455 2022-12-31T06:58:26Z Brewer's spent grain (BSG) is a co-product of the brewing industry and is a potentially rich source of bioactive peptides. A BSG protein hydrolysate, manufactured at a semi-pilot scale, mediated significant hypotensive effects 6 h after ingestion in spontaneously hypertensive rats. This hydrolysate was fractionated and further analysed by UPLC-ESI-MS/MS for peptide identification. The peptides, IPY and LPY demonstrated the highest antioxidant activity (0.37 ± 0.02 and 0.34 ± 0.02 µmol Trolox equivalents µM peptide−1, respectively), IPLQP and LPLQP had the highest ACE inhibitory activity (IC50: 3.10 ± 0.60 and 3.17 ± 0.60 µM, respectively) while IPVP had the highest DPP-IV inhibitory activity (IC50: 38.67 ± 5.94 µM). Overall, peptides containing I had higher in vitro bioactivities when compared to peptides having L. Novel BSG peptides were identified with potential for application as naturally derived ingredients for the management of cardiovascular disease and type 2 diabetes. Article in Journal/Newspaper IPY Directory of Open Access Journals: DOAJ Articles Journal of Functional Foods 60 103455 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Brewers spent grain Hypotensive Antioxidant Peptide ACE DPP-IV Nutrition. Foods and food supply TX341-641 |
spellingShingle |
Brewers spent grain Hypotensive Antioxidant Peptide ACE DPP-IV Nutrition. Foods and food supply TX341-641 Maria Cermeño Alan Connolly Martina B. O'Keeffe Cal Flynn Adeola M. Alashi Rotimi E. Aluko Richard J. FitzGerald Identification of bioactive peptides from brewers’ spent grain and contribution of Leu/Ile to bioactive potency |
topic_facet |
Brewers spent grain Hypotensive Antioxidant Peptide ACE DPP-IV Nutrition. Foods and food supply TX341-641 |
description |
Brewer's spent grain (BSG) is a co-product of the brewing industry and is a potentially rich source of bioactive peptides. A BSG protein hydrolysate, manufactured at a semi-pilot scale, mediated significant hypotensive effects 6 h after ingestion in spontaneously hypertensive rats. This hydrolysate was fractionated and further analysed by UPLC-ESI-MS/MS for peptide identification. The peptides, IPY and LPY demonstrated the highest antioxidant activity (0.37 ± 0.02 and 0.34 ± 0.02 µmol Trolox equivalents µM peptide−1, respectively), IPLQP and LPLQP had the highest ACE inhibitory activity (IC50: 3.10 ± 0.60 and 3.17 ± 0.60 µM, respectively) while IPVP had the highest DPP-IV inhibitory activity (IC50: 38.67 ± 5.94 µM). Overall, peptides containing I had higher in vitro bioactivities when compared to peptides having L. Novel BSG peptides were identified with potential for application as naturally derived ingredients for the management of cardiovascular disease and type 2 diabetes. |
format |
Article in Journal/Newspaper |
author |
Maria Cermeño Alan Connolly Martina B. O'Keeffe Cal Flynn Adeola M. Alashi Rotimi E. Aluko Richard J. FitzGerald |
author_facet |
Maria Cermeño Alan Connolly Martina B. O'Keeffe Cal Flynn Adeola M. Alashi Rotimi E. Aluko Richard J. FitzGerald |
author_sort |
Maria Cermeño |
title |
Identification of bioactive peptides from brewers’ spent grain and contribution of Leu/Ile to bioactive potency |
title_short |
Identification of bioactive peptides from brewers’ spent grain and contribution of Leu/Ile to bioactive potency |
title_full |
Identification of bioactive peptides from brewers’ spent grain and contribution of Leu/Ile to bioactive potency |
title_fullStr |
Identification of bioactive peptides from brewers’ spent grain and contribution of Leu/Ile to bioactive potency |
title_full_unstemmed |
Identification of bioactive peptides from brewers’ spent grain and contribution of Leu/Ile to bioactive potency |
title_sort |
identification of bioactive peptides from brewers’ spent grain and contribution of leu/ile to bioactive potency |
publisher |
Elsevier |
publishDate |
2019 |
url |
https://doi.org/10.1016/j.jff.2019.103455 https://doaj.org/article/71838990c9e643e28c652311fda61fe0 |
genre |
IPY |
genre_facet |
IPY |
op_source |
Journal of Functional Foods, Vol 60, Iss , Pp - (2019) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S1756464619303792 https://doaj.org/toc/1756-4646 1756-4646 doi:10.1016/j.jff.2019.103455 https://doaj.org/article/71838990c9e643e28c652311fda61fe0 |
op_doi |
https://doi.org/10.1016/j.jff.2019.103455 |
container_title |
Journal of Functional Foods |
container_volume |
60 |
container_start_page |
103455 |
_version_ |
1766046891779293184 |