Molecular surveillance of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum isolates from Mozambique
Abstract Background Malaria programmes use Plasmodium falciparum histidine-rich protein-2 (PfHRP2) based rapid diagnostic tests (RDTs) for malaria diagnosis. The deletion of this target antigen could potentially lead to misdiagnosis, delayed treatment and continuation of active transmission. Methods...
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ftdoajarticles:oai:doaj.org/article:717429bc75324f27b6d449cfa9344ae6 2023-05-15T15:04:08+02:00 Molecular surveillance of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum isolates from Mozambique Himanshu Gupta Gloria Matambisso Beatriz Galatas Pau Cisteró Lidia Nhamussua Wilson Simone Jane Cunningham N. Regina Rabinovich Pedro Alonso Francisco Saute Pedro Aide Alfredo Mayor 2017-10-01T00:00:00Z https://doi.org/10.1186/s12936-017-2061-z https://doaj.org/article/717429bc75324f27b6d449cfa9344ae6 EN eng BMC http://link.springer.com/article/10.1186/s12936-017-2061-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-2061-z 1475-2875 https://doaj.org/article/717429bc75324f27b6d449cfa9344ae6 Malaria Journal, Vol 16, Iss 1, Pp 1-7 (2017) Malaria Deletion RDT Mozambique Pfhrp2 Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-2061-z 2022-12-31T13:25:23Z Abstract Background Malaria programmes use Plasmodium falciparum histidine-rich protein-2 (PfHRP2) based rapid diagnostic tests (RDTs) for malaria diagnosis. The deletion of this target antigen could potentially lead to misdiagnosis, delayed treatment and continuation of active transmission. Methods Plasmodium falciparum isolates (n = 1162) collected in Southern Mozambique were assessed by RDTs, microscopy and/or 18SrRNA qPCR. pfhrp2 and pfhrp3 deletions were investigated in isolates from individuals who were negative by RDT but positive by microscopy and/or qPCR (n = 69) using gene-specific PCRs, with kelch13 PCR as the parasite DNA control. Results Lack of pfhrp2 PCR amplification was observed in one of the 69 isolates subjected to molecular analysis [1.45% (95% CI 0.3–7.8%)]. Conclusions The low prevalence of pfhrp2 deletions suggests that RDTs will detect the vast majority of the P. falciparum infections. Nevertheless, active surveillance for changing deletion frequencies is required. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Malaria Deletion RDT Mozambique Pfhrp2 Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria Deletion RDT Mozambique Pfhrp2 Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Himanshu Gupta Gloria Matambisso Beatriz Galatas Pau Cisteró Lidia Nhamussua Wilson Simone Jane Cunningham N. Regina Rabinovich Pedro Alonso Francisco Saute Pedro Aide Alfredo Mayor Molecular surveillance of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum isolates from Mozambique |
topic_facet |
Malaria Deletion RDT Mozambique Pfhrp2 Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria programmes use Plasmodium falciparum histidine-rich protein-2 (PfHRP2) based rapid diagnostic tests (RDTs) for malaria diagnosis. The deletion of this target antigen could potentially lead to misdiagnosis, delayed treatment and continuation of active transmission. Methods Plasmodium falciparum isolates (n = 1162) collected in Southern Mozambique were assessed by RDTs, microscopy and/or 18SrRNA qPCR. pfhrp2 and pfhrp3 deletions were investigated in isolates from individuals who were negative by RDT but positive by microscopy and/or qPCR (n = 69) using gene-specific PCRs, with kelch13 PCR as the parasite DNA control. Results Lack of pfhrp2 PCR amplification was observed in one of the 69 isolates subjected to molecular analysis [1.45% (95% CI 0.3–7.8%)]. Conclusions The low prevalence of pfhrp2 deletions suggests that RDTs will detect the vast majority of the P. falciparum infections. Nevertheless, active surveillance for changing deletion frequencies is required. |
format |
Article in Journal/Newspaper |
author |
Himanshu Gupta Gloria Matambisso Beatriz Galatas Pau Cisteró Lidia Nhamussua Wilson Simone Jane Cunningham N. Regina Rabinovich Pedro Alonso Francisco Saute Pedro Aide Alfredo Mayor |
author_facet |
Himanshu Gupta Gloria Matambisso Beatriz Galatas Pau Cisteró Lidia Nhamussua Wilson Simone Jane Cunningham N. Regina Rabinovich Pedro Alonso Francisco Saute Pedro Aide Alfredo Mayor |
author_sort |
Himanshu Gupta |
title |
Molecular surveillance of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum isolates from Mozambique |
title_short |
Molecular surveillance of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum isolates from Mozambique |
title_full |
Molecular surveillance of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum isolates from Mozambique |
title_fullStr |
Molecular surveillance of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum isolates from Mozambique |
title_full_unstemmed |
Molecular surveillance of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum isolates from Mozambique |
title_sort |
molecular surveillance of pfhrp2 and pfhrp3 deletions in plasmodium falciparum isolates from mozambique |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-2061-z https://doaj.org/article/717429bc75324f27b6d449cfa9344ae6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-7 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-2061-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-2061-z 1475-2875 https://doaj.org/article/717429bc75324f27b6d449cfa9344ae6 |
op_doi |
https://doi.org/10.1186/s12936-017-2061-z |
container_title |
Malaria Journal |
container_volume |
16 |
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1 |
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1766335956535738368 |