Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin
Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well st...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:71437bcf2f60477bacb7a8aa8db9743f 2023-05-15T15:09:37+02:00 Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin G. Rádis-Baptista F. B. M. B. Moreno L. L. Nogueira A. M. C. Martins D. O. Toyama M. H. Toyama W. F. Azevedo Jr B. S. Cavada T. Yamane 2005-12-01T00:00:00Z https://doi.org/10.1590/S1678-91992005000400013 https://doaj.org/article/71437bcf2f60477bacb7a8aa8db9743f EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400013 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992005000400013 1678-9199 https://doaj.org/article/71437bcf2f60477bacb7a8aa8db9743f Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 11, Iss 4, Pp 557-578 (2005) Crotalus durisssus venom snake venom C-type lectin homology modeling platelet aggregation antimicrobial activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2005 ftdoajarticles https://doi.org/10.1590/S1678-91992005000400013 2022-12-31T00:35:12Z Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen receptor. Several cDNA precursors, homolog of CVX subunits, were cloned by PCR homology screening. As determined by computational analysis, one of them, named crotacetin beta subunit, was predicted as a polypeptide with a tridimensional conformation very similar to other subunits of convulxin-like snake toxins. Crotacetin was purified from C. durissus venoms by gel permeation and reverse phase high performance liquid chromatography. The heterodimeric crotacetin is expressed in the venoms of several C. durissus subspecies, but it is prevalent in the venom of C. durissus cascavella. As inferred from homology modeling, crotacetin induces platelet aggregation but noticeably exhibits antimicrobial activity against Gram-positive and Gram-negative bacteria. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 11 4 |
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topic |
Crotalus durisssus venom snake venom C-type lectin homology modeling platelet aggregation antimicrobial activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Crotalus durisssus venom snake venom C-type lectin homology modeling platelet aggregation antimicrobial activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 G. Rádis-Baptista F. B. M. B. Moreno L. L. Nogueira A. M. C. Martins D. O. Toyama M. H. Toyama W. F. Azevedo Jr B. S. Cavada T. Yamane Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
topic_facet |
Crotalus durisssus venom snake venom C-type lectin homology modeling platelet aggregation antimicrobial activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen receptor. Several cDNA precursors, homolog of CVX subunits, were cloned by PCR homology screening. As determined by computational analysis, one of them, named crotacetin beta subunit, was predicted as a polypeptide with a tridimensional conformation very similar to other subunits of convulxin-like snake toxins. Crotacetin was purified from C. durissus venoms by gel permeation and reverse phase high performance liquid chromatography. The heterodimeric crotacetin is expressed in the venoms of several C. durissus subspecies, but it is prevalent in the venom of C. durissus cascavella. As inferred from homology modeling, crotacetin induces platelet aggregation but noticeably exhibits antimicrobial activity against Gram-positive and Gram-negative bacteria. |
format |
Article in Journal/Newspaper |
author |
G. Rádis-Baptista F. B. M. B. Moreno L. L. Nogueira A. M. C. Martins D. O. Toyama M. H. Toyama W. F. Azevedo Jr B. S. Cavada T. Yamane |
author_facet |
G. Rádis-Baptista F. B. M. B. Moreno L. L. Nogueira A. M. C. Martins D. O. Toyama M. H. Toyama W. F. Azevedo Jr B. S. Cavada T. Yamane |
author_sort |
G. Rádis-Baptista |
title |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title_short |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title_full |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title_fullStr |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title_full_unstemmed |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title_sort |
crotacetin, a novel snake venom c-type lectin, is homolog of convulxin |
publisher |
SciELO |
publishDate |
2005 |
url |
https://doi.org/10.1590/S1678-91992005000400013 https://doaj.org/article/71437bcf2f60477bacb7a8aa8db9743f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 11, Iss 4, Pp 557-578 (2005) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400013 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992005000400013 1678-9199 https://doaj.org/article/71437bcf2f60477bacb7a8aa8db9743f |
op_doi |
https://doi.org/10.1590/S1678-91992005000400013 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
11 |
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4 |
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1766340778496360448 |