Plasmodium vivax infection in Anajás, State of Pará: no differential resistance profile among Duffy-negative and Duffy-positive individuals
Abstract Background There is large body of evidence that states that invasion of Plasmodium vivax requires the Duffy antigen, but the universality of this specificity is certainly now under question with recent reports showing that in some parts of the world P . vivax infects and causes disease in D...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2012
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1475-2875-11-430 https://doaj.org/article/713730be3300461f8a41ebca285a9e91 |
id |
ftdoajarticles:oai:doaj.org/article:713730be3300461f8a41ebca285a9e91 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:713730be3300461f8a41ebca285a9e91 2023-05-15T15:17:26+02:00 Plasmodium vivax infection in Anajás, State of Pará: no differential resistance profile among Duffy-negative and Duffy-positive individuals Carvalho Tarcisio AA Queiroz Maíse G Cardoso Greice L Diniz Isabela G Silva Aylla NLM Pinto Ana YN Guerreiro João F 2012-12-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-430 https://doaj.org/article/713730be3300461f8a41ebca285a9e91 EN eng BMC http://www.malariajournal.com/content/11/1/430 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-430 1475-2875 https://doaj.org/article/713730be3300461f8a41ebca285a9e91 Malaria Journal, Vol 11, Iss 1, p 430 (2012) Vivax malaria Duffy blood group Brazilian Amazonia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-430 2022-12-31T00:47:17Z Abstract Background There is large body of evidence that states that invasion of Plasmodium vivax requires the Duffy antigen, but the universality of this specificity is certainly now under question with recent reports showing that in some parts of the world P . vivax infects and causes disease in Duffy-negative people. These findings reinforce the idea that this parasite is rapidly evolving, being able to use other receptors than Duffy to invade the erythrocytes, which may have an enormous impact in P . vivax current distribution. The presence of P . vivax infection in Duffy-negative individuals was investigated in a cross-sectional study conducted in Anajás, Archipelago of Marajó, State of Pará, which is an area of malaria transmission in the Brazilian Amazonia. Methods Duffy genotyping and Plasmodium species diagnostic assays were performed successfully in 678 individuals. An allele-specific primer polymerase chain reaction (PCR) technique was used for Duffy blood group genotyping. Identification of Plasmodium species was achieved by conventional blood smear light microscopy and a TaqMan-based real-time PCR method to detect mitochondrial genome of Plasmodium falciparum and P . vivax . Results Plasmodium spp . infection was detected in 137 samples (20.2%). Prevalence of each Plasmodium species was 13.9% P . vivax , 5.8% P . falciparum , and 0.6% P . vivax plus P . falciparum . Overall, 4.3% (29/678) were genotyped as Duffy-negative ( FY * B ES /* B ES ). Among Duffy-negative individuals 6.9% were P . vivax PCR positive and among Duffy-positive 14.2% were P . vivax PCR positive. Although lower, the risk of Duffy-negatives to experience a P . vivax blood stage infection was not significantly different to that of Duffy-positives. Furthermore, the genotypic and allelic frequencies of the Duffy blood group among P . vivax -infected patients and in the control group did not differ significantly, also suggesting no reduction in infection rates among the carriers of FY * B ES allele. Conclusions The data obtained in ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 430 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Vivax malaria Duffy blood group Brazilian Amazonia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Vivax malaria Duffy blood group Brazilian Amazonia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Carvalho Tarcisio AA Queiroz Maíse G Cardoso Greice L Diniz Isabela G Silva Aylla NLM Pinto Ana YN Guerreiro João F Plasmodium vivax infection in Anajás, State of Pará: no differential resistance profile among Duffy-negative and Duffy-positive individuals |
topic_facet |
Vivax malaria Duffy blood group Brazilian Amazonia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background There is large body of evidence that states that invasion of Plasmodium vivax requires the Duffy antigen, but the universality of this specificity is certainly now under question with recent reports showing that in some parts of the world P . vivax infects and causes disease in Duffy-negative people. These findings reinforce the idea that this parasite is rapidly evolving, being able to use other receptors than Duffy to invade the erythrocytes, which may have an enormous impact in P . vivax current distribution. The presence of P . vivax infection in Duffy-negative individuals was investigated in a cross-sectional study conducted in Anajás, Archipelago of Marajó, State of Pará, which is an area of malaria transmission in the Brazilian Amazonia. Methods Duffy genotyping and Plasmodium species diagnostic assays were performed successfully in 678 individuals. An allele-specific primer polymerase chain reaction (PCR) technique was used for Duffy blood group genotyping. Identification of Plasmodium species was achieved by conventional blood smear light microscopy and a TaqMan-based real-time PCR method to detect mitochondrial genome of Plasmodium falciparum and P . vivax . Results Plasmodium spp . infection was detected in 137 samples (20.2%). Prevalence of each Plasmodium species was 13.9% P . vivax , 5.8% P . falciparum , and 0.6% P . vivax plus P . falciparum . Overall, 4.3% (29/678) were genotyped as Duffy-negative ( FY * B ES /* B ES ). Among Duffy-negative individuals 6.9% were P . vivax PCR positive and among Duffy-positive 14.2% were P . vivax PCR positive. Although lower, the risk of Duffy-negatives to experience a P . vivax blood stage infection was not significantly different to that of Duffy-positives. Furthermore, the genotypic and allelic frequencies of the Duffy blood group among P . vivax -infected patients and in the control group did not differ significantly, also suggesting no reduction in infection rates among the carriers of FY * B ES allele. Conclusions The data obtained in ... |
format |
Article in Journal/Newspaper |
author |
Carvalho Tarcisio AA Queiroz Maíse G Cardoso Greice L Diniz Isabela G Silva Aylla NLM Pinto Ana YN Guerreiro João F |
author_facet |
Carvalho Tarcisio AA Queiroz Maíse G Cardoso Greice L Diniz Isabela G Silva Aylla NLM Pinto Ana YN Guerreiro João F |
author_sort |
Carvalho Tarcisio AA |
title |
Plasmodium vivax infection in Anajás, State of Pará: no differential resistance profile among Duffy-negative and Duffy-positive individuals |
title_short |
Plasmodium vivax infection in Anajás, State of Pará: no differential resistance profile among Duffy-negative and Duffy-positive individuals |
title_full |
Plasmodium vivax infection in Anajás, State of Pará: no differential resistance profile among Duffy-negative and Duffy-positive individuals |
title_fullStr |
Plasmodium vivax infection in Anajás, State of Pará: no differential resistance profile among Duffy-negative and Duffy-positive individuals |
title_full_unstemmed |
Plasmodium vivax infection in Anajás, State of Pará: no differential resistance profile among Duffy-negative and Duffy-positive individuals |
title_sort |
plasmodium vivax infection in anajás, state of pará: no differential resistance profile among duffy-negative and duffy-positive individuals |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-430 https://doaj.org/article/713730be3300461f8a41ebca285a9e91 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 430 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/430 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-430 1475-2875 https://doaj.org/article/713730be3300461f8a41ebca285a9e91 |
op_doi |
https://doi.org/10.1186/1475-2875-11-430 |
container_title |
Malaria Journal |
container_volume |
11 |
container_issue |
1 |
container_start_page |
430 |
_version_ |
1766347677495197696 |