Phenotypic and Molecular Detection of Methicillin Resistance in S. aureus
Detecting methicillin resistance is complicated due to the heterogeneity of its phenotypic expression, making its detection difficult in the laboratory; this has led to the development of several techniques to increase its expression in vitro. Four techniques for detecting methicillin resistance wer...
Main Authors: | , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English Spanish |
Published: |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2008
|
Subjects: | |
Online Access: | https://doaj.org/article/712c362d621c413ebe8652ca91f45f31 |
id |
ftdoajarticles:oai:doaj.org/article:712c362d621c413ebe8652ca91f45f31 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:712c362d621c413ebe8652ca91f45f31 2023-10-01T03:54:04+02:00 Phenotypic and Molecular Detection of Methicillin Resistance in S. aureus Maribel Castellano González Armindo Perozo Mena Rosana Vivas Vega 2008-06-01T00:00:00Z https://doaj.org/article/712c362d621c413ebe8652ca91f45f31 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://produccioncientificaluz.org/index.php/kasmera/article/view/4820 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/712c362d621c413ebe8652ca91f45f31 Kasmera, Vol 36, Iss 1, Pp 28-38 (2008) S. aureus resistencia ameticilina métodos fenotípicos,métodos moleculares Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2008 ftdoajarticles 2023-09-03T00:47:34Z Detecting methicillin resistance is complicated due to the heterogeneity of its phenotypic expression, making its detection difficult in the laboratory; this has led to the development of several techniques to increase its expression in vitro. Four techniques for detecting methicillin resistance were evaluated: the disk diffusion method with oxacillin (OX, 1 μg) and cephoxitin (FOX, 30 μg); the screening test, minimum inhibitory concentration (MIC) and detection of PBP2a, using the presence of mecA gen as a reference method; 286 strains of S. aureus, were processed. The sensibility (SEN), specificity (SPE), positive predictive value (PPV), negative predictive value (NPV) and efficiency (EFC) of each method were determined. A total of 50 oxacillin resistant, PBP2a positive (mecA positive) strains were obtained. Sensibility of the OX disk was 99.14%; and of the FOX disk was 100%. The SEN, PPV, NVP and EFC of the other methods were 100%. All the tests, except the OX disk diffusion method (99.14% of ESP), were 100% specific. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
S. aureus resistencia ameticilina métodos fenotípicos,métodos moleculares Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
S. aureus resistencia ameticilina métodos fenotípicos,métodos moleculares Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Maribel Castellano González Armindo Perozo Mena Rosana Vivas Vega Phenotypic and Molecular Detection of Methicillin Resistance in S. aureus |
topic_facet |
S. aureus resistencia ameticilina métodos fenotípicos,métodos moleculares Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Detecting methicillin resistance is complicated due to the heterogeneity of its phenotypic expression, making its detection difficult in the laboratory; this has led to the development of several techniques to increase its expression in vitro. Four techniques for detecting methicillin resistance were evaluated: the disk diffusion method with oxacillin (OX, 1 μg) and cephoxitin (FOX, 30 μg); the screening test, minimum inhibitory concentration (MIC) and detection of PBP2a, using the presence of mecA gen as a reference method; 286 strains of S. aureus, were processed. The sensibility (SEN), specificity (SPE), positive predictive value (PPV), negative predictive value (NPV) and efficiency (EFC) of each method were determined. A total of 50 oxacillin resistant, PBP2a positive (mecA positive) strains were obtained. Sensibility of the OX disk was 99.14%; and of the FOX disk was 100%. The SEN, PPV, NVP and EFC of the other methods were 100%. All the tests, except the OX disk diffusion method (99.14% of ESP), were 100% specific. |
format |
Article in Journal/Newspaper |
author |
Maribel Castellano González Armindo Perozo Mena Rosana Vivas Vega |
author_facet |
Maribel Castellano González Armindo Perozo Mena Rosana Vivas Vega |
author_sort |
Maribel Castellano González |
title |
Phenotypic and Molecular Detection of Methicillin Resistance in S. aureus |
title_short |
Phenotypic and Molecular Detection of Methicillin Resistance in S. aureus |
title_full |
Phenotypic and Molecular Detection of Methicillin Resistance in S. aureus |
title_fullStr |
Phenotypic and Molecular Detection of Methicillin Resistance in S. aureus |
title_full_unstemmed |
Phenotypic and Molecular Detection of Methicillin Resistance in S. aureus |
title_sort |
phenotypic and molecular detection of methicillin resistance in s. aureus |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2008 |
url |
https://doaj.org/article/712c362d621c413ebe8652ca91f45f31 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 36, Iss 1, Pp 28-38 (2008) |
op_relation |
https://produccioncientificaluz.org/index.php/kasmera/article/view/4820 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/712c362d621c413ebe8652ca91f45f31 |
_version_ |
1778521363461439488 |