Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study
The damaging effects of neuwiedase, a non-hemorrhagic snake venom metalloproteinase from P-I class, on gastrocnemius muscle are studied herein. Following neuwiedase injection, ultrastructural alterations were detected early showing disarrangement of skeletal muscle fibers (characterized by discontin...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/S1678-91992010000300013 https://doaj.org/article/70f08337aac74713a044f26b3e38e393 |
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ftdoajarticles:oai:doaj.org/article:70f08337aac74713a044f26b3e38e393 2023-05-15T15:04:20+02:00 Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study C Baldo MJ Ferreira DS Lopes LFM Izidoro AO Gomes EAV Ferro A Hamaguchi MI Homsi-Brandeburgo VM Rodrigues 2010-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992010000300013 https://doaj.org/article/70f08337aac74713a044f26b3e38e393 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300013 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000300013 1678-9199 https://doaj.org/article/70f08337aac74713a044f26b3e38e393 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 3, Pp 462-469 (2010) skeletal muscle neuwiedase snake venom metalloproteinases Bothrops neuwiedi Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2010 ftdoajarticles https://doi.org/10.1590/S1678-91992010000300013 2022-12-31T03:30:21Z The damaging effects of neuwiedase, a non-hemorrhagic snake venom metalloproteinase from P-I class, on gastrocnemius muscle are studied herein. Following neuwiedase injection, ultrastructural alterations were detected early showing disarrangement of skeletal muscle fibers (characterized by discontinuity of Z lines), mitochondrial swelling, and disruption of plasma membrane and basal lamina. Degradation of skeletal muscle and the appearance of an amorphous substance, primarily composed of cellular debris, were noted after 24 hours. The presence of neuwiedase at the injection site (detected by immunocytochemistry) revealed highly specific labeling of myofibril components of damaged myocytes. In addition, proteolysis of muscle proteins assayed through myofibrils extracted from gastrocnemius muscle indicated that neuwiedase provoked degradation of myofibrils, especially myosin. These results suggest that skeletal muscle damage, induced by neuwiedase, is probably due to its proteolytic action on myofibrils, which are responsible for the maintenance of the cellular architecture. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 16 3 462 469 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
skeletal muscle neuwiedase snake venom metalloproteinases Bothrops neuwiedi Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
skeletal muscle neuwiedase snake venom metalloproteinases Bothrops neuwiedi Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 C Baldo MJ Ferreira DS Lopes LFM Izidoro AO Gomes EAV Ferro A Hamaguchi MI Homsi-Brandeburgo VM Rodrigues Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study |
topic_facet |
skeletal muscle neuwiedase snake venom metalloproteinases Bothrops neuwiedi Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
The damaging effects of neuwiedase, a non-hemorrhagic snake venom metalloproteinase from P-I class, on gastrocnemius muscle are studied herein. Following neuwiedase injection, ultrastructural alterations were detected early showing disarrangement of skeletal muscle fibers (characterized by discontinuity of Z lines), mitochondrial swelling, and disruption of plasma membrane and basal lamina. Degradation of skeletal muscle and the appearance of an amorphous substance, primarily composed of cellular debris, were noted after 24 hours. The presence of neuwiedase at the injection site (detected by immunocytochemistry) revealed highly specific labeling of myofibril components of damaged myocytes. In addition, proteolysis of muscle proteins assayed through myofibrils extracted from gastrocnemius muscle indicated that neuwiedase provoked degradation of myofibrils, especially myosin. These results suggest that skeletal muscle damage, induced by neuwiedase, is probably due to its proteolytic action on myofibrils, which are responsible for the maintenance of the cellular architecture. |
format |
Article in Journal/Newspaper |
author |
C Baldo MJ Ferreira DS Lopes LFM Izidoro AO Gomes EAV Ferro A Hamaguchi MI Homsi-Brandeburgo VM Rodrigues |
author_facet |
C Baldo MJ Ferreira DS Lopes LFM Izidoro AO Gomes EAV Ferro A Hamaguchi MI Homsi-Brandeburgo VM Rodrigues |
author_sort |
C Baldo |
title |
Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study |
title_short |
Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study |
title_full |
Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study |
title_fullStr |
Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study |
title_full_unstemmed |
Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study |
title_sort |
action of neuwiedase, a metalloproteinase isolated from bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study |
publisher |
SciELO |
publishDate |
2010 |
url |
https://doi.org/10.1590/S1678-91992010000300013 https://doaj.org/article/70f08337aac74713a044f26b3e38e393 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 3, Pp 462-469 (2010) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300013 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000300013 1678-9199 https://doaj.org/article/70f08337aac74713a044f26b3e38e393 |
op_doi |
https://doi.org/10.1590/S1678-91992010000300013 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
16 |
container_issue |
3 |
container_start_page |
462 |
op_container_end_page |
469 |
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1766336123769978880 |