Identification and characterization of a Mef2 transcriptional activator in schistosome parasites.
Myocyte enhancer factor 2 protein (Mef2) is an evolutionarily conserved activator of transcription that is critical to induce and control complex processes in myogenesis and neurogenesis in vertebrates and insects, and osteogenesis in vertebrates. In Drosophila, Mef2 null mutants are unable to produ...
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ftdoajarticles:oai:doaj.org/article:708edea040ae4f22809c4bf2cb6b1b2a 2023-05-15T15:06:06+02:00 Identification and characterization of a Mef2 transcriptional activator in schistosome parasites. John N Milligan Emmitt R Jolly 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001443 https://doaj.org/article/708edea040ae4f22809c4bf2cb6b1b2a EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3250504?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001443 https://doaj.org/article/708edea040ae4f22809c4bf2cb6b1b2a PLoS Neglected Tropical Diseases, Vol 6, Iss 1, p e1443 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001443 2022-12-30T21:34:17Z Myocyte enhancer factor 2 protein (Mef2) is an evolutionarily conserved activator of transcription that is critical to induce and control complex processes in myogenesis and neurogenesis in vertebrates and insects, and osteogenesis in vertebrates. In Drosophila, Mef2 null mutants are unable to produce differentiated muscle cells, and in vertebrates, Mef2 mutants are embryonic lethal. Schistosome worms are responsible for over 200 million cases of schistosomiasis globally, but little is known about early development of schistosome parasites after infecting a vertebrate host. Understanding basic schistosome development could be crucial to delineating potential drug targets. Here, we identify and characterize Mef2 from the schistosome worm Schistosoma mansoni (SmMef2). We initially identified SmMef2 as a homolog to the yeast Mef2 homolog, Resistance to Lethality of MKK1P386 overexpression (Rlm1), and we show that SmMef2 is homologous to conserved Mef2 family proteins. Using a genetics approach, we demonstrate that SmMef2 is a transactivator that can induce transcription of four separate heterologous reporter genes by yeast one-hybrid analysis. We also show that Mef2 is expressed during several stages of schistosome development by quantitative PCR and that it can bind to conserved Mef2 DNA consensus binding sequences. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 1 e1443 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 John N Milligan Emmitt R Jolly Identification and characterization of a Mef2 transcriptional activator in schistosome parasites. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Myocyte enhancer factor 2 protein (Mef2) is an evolutionarily conserved activator of transcription that is critical to induce and control complex processes in myogenesis and neurogenesis in vertebrates and insects, and osteogenesis in vertebrates. In Drosophila, Mef2 null mutants are unable to produce differentiated muscle cells, and in vertebrates, Mef2 mutants are embryonic lethal. Schistosome worms are responsible for over 200 million cases of schistosomiasis globally, but little is known about early development of schistosome parasites after infecting a vertebrate host. Understanding basic schistosome development could be crucial to delineating potential drug targets. Here, we identify and characterize Mef2 from the schistosome worm Schistosoma mansoni (SmMef2). We initially identified SmMef2 as a homolog to the yeast Mef2 homolog, Resistance to Lethality of MKK1P386 overexpression (Rlm1), and we show that SmMef2 is homologous to conserved Mef2 family proteins. Using a genetics approach, we demonstrate that SmMef2 is a transactivator that can induce transcription of four separate heterologous reporter genes by yeast one-hybrid analysis. We also show that Mef2 is expressed during several stages of schistosome development by quantitative PCR and that it can bind to conserved Mef2 DNA consensus binding sequences. |
format |
Article in Journal/Newspaper |
author |
John N Milligan Emmitt R Jolly |
author_facet |
John N Milligan Emmitt R Jolly |
author_sort |
John N Milligan |
title |
Identification and characterization of a Mef2 transcriptional activator in schistosome parasites. |
title_short |
Identification and characterization of a Mef2 transcriptional activator in schistosome parasites. |
title_full |
Identification and characterization of a Mef2 transcriptional activator in schistosome parasites. |
title_fullStr |
Identification and characterization of a Mef2 transcriptional activator in schistosome parasites. |
title_full_unstemmed |
Identification and characterization of a Mef2 transcriptional activator in schistosome parasites. |
title_sort |
identification and characterization of a mef2 transcriptional activator in schistosome parasites. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001443 https://doaj.org/article/708edea040ae4f22809c4bf2cb6b1b2a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 1, p e1443 (2012) |
op_relation |
http://europepmc.org/articles/PMC3250504?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001443 https://doaj.org/article/708edea040ae4f22809c4bf2cb6b1b2a |
op_doi |
https://doi.org/10.1371/journal.pntd.0001443 |
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PLoS Neglected Tropical Diseases |
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6 |
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1 |
container_start_page |
e1443 |
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1766337764680269824 |