A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain
Microbial communities inhabiting the Antarctic Ocean show psychrophilic and halophilic adaptations conferring interesting properties to the enzymes they produce, which could be exploited in biotechnology and bioremediation processes. Use of cold- and salt-tolerant enzymes allows to limit costs, redu...
Published in: | Frontiers in Microbiology |
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2023
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Online Access: | https://doi.org/10.3389/fmicb.2023.1078382 https://doaj.org/article/70395d18e4644bbeaa56834a1a999cb7 |
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ftdoajarticles:oai:doaj.org/article:70395d18e4644bbeaa56834a1a999cb7 2023-05-15T14:01:11+02:00 A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain Melissa Bisaccia Elisa Binda Elena Rosini Gabriella Caruso Ombretta Dell'Acqua Maurizio Azzaro Pasqualina Laganà Gabriella Tedeschi Elisa M. Maffioli Loredano Pollegioni Flavia Marinelli 2023-02-01T00:00:00Z https://doi.org/10.3389/fmicb.2023.1078382 https://doaj.org/article/70395d18e4644bbeaa56834a1a999cb7 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1078382/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2023.1078382 https://doaj.org/article/70395d18e4644bbeaa56834a1a999cb7 Frontiers in Microbiology, Vol 14 (2023) Antarctica laccase Halomonas sp marine biofilm cold-adaptation extremophile bacteria Microbiology QR1-502 article 2023 ftdoajarticles https://doi.org/10.3389/fmicb.2023.1078382 2023-02-12T01:27:51Z Microbial communities inhabiting the Antarctic Ocean show psychrophilic and halophilic adaptations conferring interesting properties to the enzymes they produce, which could be exploited in biotechnology and bioremediation processes. Use of cold- and salt-tolerant enzymes allows to limit costs, reduce contaminations, and minimize pretreatment steps. Here, we report on the screening of 186 morphologically diverse microorganisms isolated from marine biofilms and water samples collected in Terra Nova Bay (Ross Sea, Antarctica) for the identification of new laccase activities. After primary screening, 13.4 and 10.8% of the isolates were identified for the ability to oxidize 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and the dye azure B, respectively. Amongst them, the marine Halomonas sp. strain M68 showed the highest activity. Production of its laccase-like activity increased six-fold when copper was added to culture medium. Enzymatic activity-guided separation coupled with mass spectrometry identified this intracellular laccase-like protein (named Ant laccase) as belonging to the copper resistance system multicopper oxidase family. Ant laccase oxidized ABTS and 2,6-dimethoxy phenol, working better at acidic pHs The enzyme showed a good thermostability, with optimal temperature in the 40–50°C range and maintaining more than 40% of its maximal activity even at 10°C. Furthermore, Ant laccase was salt- and organic solvent-tolerant, paving the way for its use in harsh conditions. To our knowledge, this is the first report concerning the characterization of a thermo- and halo-tolerant laccase isolated from a marine Antarctic bacterium. Article in Journal/Newspaper Antarc* Antarctic Antarctic Ocean Antarctica Ross Sea Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Ross Sea Terra Nova Bay Antarctic Ocean Frontiers in Microbiology 14 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctica laccase Halomonas sp marine biofilm cold-adaptation extremophile bacteria Microbiology QR1-502 |
spellingShingle |
Antarctica laccase Halomonas sp marine biofilm cold-adaptation extremophile bacteria Microbiology QR1-502 Melissa Bisaccia Elisa Binda Elena Rosini Gabriella Caruso Ombretta Dell'Acqua Maurizio Azzaro Pasqualina Laganà Gabriella Tedeschi Elisa M. Maffioli Loredano Pollegioni Flavia Marinelli A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain |
topic_facet |
Antarctica laccase Halomonas sp marine biofilm cold-adaptation extremophile bacteria Microbiology QR1-502 |
description |
Microbial communities inhabiting the Antarctic Ocean show psychrophilic and halophilic adaptations conferring interesting properties to the enzymes they produce, which could be exploited in biotechnology and bioremediation processes. Use of cold- and salt-tolerant enzymes allows to limit costs, reduce contaminations, and minimize pretreatment steps. Here, we report on the screening of 186 morphologically diverse microorganisms isolated from marine biofilms and water samples collected in Terra Nova Bay (Ross Sea, Antarctica) for the identification of new laccase activities. After primary screening, 13.4 and 10.8% of the isolates were identified for the ability to oxidize 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and the dye azure B, respectively. Amongst them, the marine Halomonas sp. strain M68 showed the highest activity. Production of its laccase-like activity increased six-fold when copper was added to culture medium. Enzymatic activity-guided separation coupled with mass spectrometry identified this intracellular laccase-like protein (named Ant laccase) as belonging to the copper resistance system multicopper oxidase family. Ant laccase oxidized ABTS and 2,6-dimethoxy phenol, working better at acidic pHs The enzyme showed a good thermostability, with optimal temperature in the 40–50°C range and maintaining more than 40% of its maximal activity even at 10°C. Furthermore, Ant laccase was salt- and organic solvent-tolerant, paving the way for its use in harsh conditions. To our knowledge, this is the first report concerning the characterization of a thermo- and halo-tolerant laccase isolated from a marine Antarctic bacterium. |
format |
Article in Journal/Newspaper |
author |
Melissa Bisaccia Elisa Binda Elena Rosini Gabriella Caruso Ombretta Dell'Acqua Maurizio Azzaro Pasqualina Laganà Gabriella Tedeschi Elisa M. Maffioli Loredano Pollegioni Flavia Marinelli |
author_facet |
Melissa Bisaccia Elisa Binda Elena Rosini Gabriella Caruso Ombretta Dell'Acqua Maurizio Azzaro Pasqualina Laganà Gabriella Tedeschi Elisa M. Maffioli Loredano Pollegioni Flavia Marinelli |
author_sort |
Melissa Bisaccia |
title |
A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain |
title_short |
A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain |
title_full |
A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain |
title_fullStr |
A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain |
title_full_unstemmed |
A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain |
title_sort |
novel promising laccase from the psychrotolerant and halotolerant antarctic marine halomonas sp. m68 strain |
publisher |
Frontiers Media S.A. |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmicb.2023.1078382 https://doaj.org/article/70395d18e4644bbeaa56834a1a999cb7 |
geographic |
Antarctic The Antarctic Ross Sea Terra Nova Bay Antarctic Ocean |
geographic_facet |
Antarctic The Antarctic Ross Sea Terra Nova Bay Antarctic Ocean |
genre |
Antarc* Antarctic Antarctic Ocean Antarctica Ross Sea |
genre_facet |
Antarc* Antarctic Antarctic Ocean Antarctica Ross Sea |
op_source |
Frontiers in Microbiology, Vol 14 (2023) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1078382/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2023.1078382 https://doaj.org/article/70395d18e4644bbeaa56834a1a999cb7 |
op_doi |
https://doi.org/10.3389/fmicb.2023.1078382 |
container_title |
Frontiers in Microbiology |
container_volume |
14 |
_version_ |
1766270784134709248 |