Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis
Colobanthus quitensis is one of the two angiosperm plant species commonly spread in the Antarctic. The species has been extensively analyzed at morphological, anatomical and physiological levels, but information regarding its genetic vari-ability remains limited. The aim of the study was to identify...
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State Institution National Antarctic Scientific Center
2020
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Online Access: | https://doi.org/10.33275/1727-7485.1.2020.382 https://doaj.org/article/7016562860d047c49f2d80fef56456a8 |
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ftdoajarticles:oai:doaj.org/article:7016562860d047c49f2d80fef56456a8 2023-12-03T10:11:54+01:00 Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis A. Rabokon A. Postovoitovа Yu. Bilonozhko L. Kalafat M. Pavlovska Ie. Prekrasna I. Parnikoza I. Kozeretska Ya. Pirko Ya. Blume 2020-07-01T00:00:00Z https://doi.org/10.33275/1727-7485.1.2020.382 https://doaj.org/article/7016562860d047c49f2d80fef56456a8 EN UK eng ukr State Institution National Antarctic Scientific Center http://uaj.uac.gov.ua/index.php/uaj/article/view/382 https://doaj.org/toc/1727-7485 https://doaj.org/toc/2415-3087 https://doi.org/10.33275/1727-7485.1.2020.382 1727-7485 2415-3087 https://doaj.org/article/7016562860d047c49f2d80fef56456a8 Український антарктичний журнал, Vol 1, Pp 93-101 (2020) colobanthus quitensis molecular genetic markers intron length polymorphism actin α-tubulin γ-tubulin Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 article 2020 ftdoajarticles https://doi.org/10.33275/1727-7485.1.2020.382 2023-11-05T01:36:31Z Colobanthus quitensis is one of the two angiosperm plant species commonly spread in the Antarctic. The species has been extensively analyzed at morphological, anatomical and physiological levels, but information regarding its genetic vari-ability remains limited. The aim of the study was to identify molecular genetic differences between C. quitensis populations in one of the Antarctic localities, the Argentine Islands region by estimating the intron length polymorphism of actin, α- and γ-tubulin genes. Samples of C. quitensis from different Antarctic natural populations were collected during the season of the 24th and previous Ukrainian Antarctic expeditions. Total DNA was isolated using the QIAGEN DNeasy Plant Mini Kit. The polymerase chain reaction was carried out with our own degenerate primers. The resulting amplicons were separated and visualized using polyacrylamide gel electrophoresis followed by silver nitrate staining. Molecular genetic analysis of natural populations of C. quitensis was performed using three DNA-marker systems based on the detection of intron length polymor-phism of actin, α- and γ-tubulin genes. A low level of genetic polymorphism of C. quitensis in the studied region by these types of markers was established. By assessing the intron length polymorphism of actin genes of the studied C. quitensis populations it was possible to establish that the populations of Skua Island had unique amplicons characteristic only for this location. This indicates the possibility of further use of the analysis of intron length polymorphism of actin genes for the study of the molecu-lar genetic diversity of the Antarctic pearlwort. At the same time, the results of analysis of the intron length polymorphism of α- and γ-tubulin genes induce selection of more specific primers, taking into account the structure of the C. quitensis genome. C. quitensis in the study region has a low level of genetic variability in intron length polymorphism of actin, α- and γ-tubulin genes. Overall, the results indicate that ... Article in Journal/Newspaper Antarc* Antarctic Argentine Islands Skua Island Directory of Open Access Journals: DOAJ Articles Antarctic Argentine Argentine Islands ENVELOPE(-64.273,-64.273,-65.246,-65.246) Skua Island ENVELOPE(-64.263,-64.263,-65.254,-65.254) The Antarctic Ukrainian Antarctic Journal 1 93 101 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English Ukrainian |
topic |
colobanthus quitensis molecular genetic markers intron length polymorphism actin α-tubulin γ-tubulin Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 |
spellingShingle |
colobanthus quitensis molecular genetic markers intron length polymorphism actin α-tubulin γ-tubulin Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 A. Rabokon A. Postovoitovа Yu. Bilonozhko L. Kalafat M. Pavlovska Ie. Prekrasna I. Parnikoza I. Kozeretska Ya. Pirko Ya. Blume Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis |
topic_facet |
colobanthus quitensis molecular genetic markers intron length polymorphism actin α-tubulin γ-tubulin Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 |
description |
Colobanthus quitensis is one of the two angiosperm plant species commonly spread in the Antarctic. The species has been extensively analyzed at morphological, anatomical and physiological levels, but information regarding its genetic vari-ability remains limited. The aim of the study was to identify molecular genetic differences between C. quitensis populations in one of the Antarctic localities, the Argentine Islands region by estimating the intron length polymorphism of actin, α- and γ-tubulin genes. Samples of C. quitensis from different Antarctic natural populations were collected during the season of the 24th and previous Ukrainian Antarctic expeditions. Total DNA was isolated using the QIAGEN DNeasy Plant Mini Kit. The polymerase chain reaction was carried out with our own degenerate primers. The resulting amplicons were separated and visualized using polyacrylamide gel electrophoresis followed by silver nitrate staining. Molecular genetic analysis of natural populations of C. quitensis was performed using three DNA-marker systems based on the detection of intron length polymor-phism of actin, α- and γ-tubulin genes. A low level of genetic polymorphism of C. quitensis in the studied region by these types of markers was established. By assessing the intron length polymorphism of actin genes of the studied C. quitensis populations it was possible to establish that the populations of Skua Island had unique amplicons characteristic only for this location. This indicates the possibility of further use of the analysis of intron length polymorphism of actin genes for the study of the molecu-lar genetic diversity of the Antarctic pearlwort. At the same time, the results of analysis of the intron length polymorphism of α- and γ-tubulin genes induce selection of more specific primers, taking into account the structure of the C. quitensis genome. C. quitensis in the study region has a low level of genetic variability in intron length polymorphism of actin, α- and γ-tubulin genes. Overall, the results indicate that ... |
format |
Article in Journal/Newspaper |
author |
A. Rabokon A. Postovoitovа Yu. Bilonozhko L. Kalafat M. Pavlovska Ie. Prekrasna I. Parnikoza I. Kozeretska Ya. Pirko Ya. Blume |
author_facet |
A. Rabokon A. Postovoitovа Yu. Bilonozhko L. Kalafat M. Pavlovska Ie. Prekrasna I. Parnikoza I. Kozeretska Ya. Pirko Ya. Blume |
author_sort |
A. Rabokon |
title |
Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis |
title_short |
Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis |
title_full |
Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis |
title_fullStr |
Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis |
title_full_unstemmed |
Assessment of Colobanthus quitensis genetic polymorphism from the Argentine Islands region (maritime Antarctic) by actin, α- and γ-tubulin gene intron analysis |
title_sort |
assessment of colobanthus quitensis genetic polymorphism from the argentine islands region (maritime antarctic) by actin, α- and γ-tubulin gene intron analysis |
publisher |
State Institution National Antarctic Scientific Center |
publishDate |
2020 |
url |
https://doi.org/10.33275/1727-7485.1.2020.382 https://doaj.org/article/7016562860d047c49f2d80fef56456a8 |
long_lat |
ENVELOPE(-64.273,-64.273,-65.246,-65.246) ENVELOPE(-64.263,-64.263,-65.254,-65.254) |
geographic |
Antarctic Argentine Argentine Islands Skua Island The Antarctic |
geographic_facet |
Antarctic Argentine Argentine Islands Skua Island The Antarctic |
genre |
Antarc* Antarctic Argentine Islands Skua Island |
genre_facet |
Antarc* Antarctic Argentine Islands Skua Island |
op_source |
Український антарктичний журнал, Vol 1, Pp 93-101 (2020) |
op_relation |
http://uaj.uac.gov.ua/index.php/uaj/article/view/382 https://doaj.org/toc/1727-7485 https://doaj.org/toc/2415-3087 https://doi.org/10.33275/1727-7485.1.2020.382 1727-7485 2415-3087 https://doaj.org/article/7016562860d047c49f2d80fef56456a8 |
op_doi |
https://doi.org/10.33275/1727-7485.1.2020.382 |
container_title |
Ukrainian Antarctic Journal |
container_issue |
1 |
container_start_page |
93 |
op_container_end_page |
101 |
_version_ |
1784257399716052992 |