Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study
Objective. To characterize carbapenemase-producing Klebsiella pneumoniae isolated from patients treated at a hospital in Cumaná, Sucre, Venezuela. Methods. This was a retrospective study conducted at the general hospital in Cumaná where 58 K. pneumoniae strains were analyzed for resistance to antimi...
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ftdoajarticles:oai:doaj.org/article:700da5ceb79e4b97a85a78dbc46516f1 2023-05-15T15:07:56+02:00 Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study Dianny Martínez Luisa Caña Hectorina Rodulfo José García Diorelis González Lucy Rodríguez Marcos De Donato 2020-09-01T00:00:00Z https://doi.org/10.26633/RPSP.2020.50 https://doaj.org/article/700da5ceb79e4b97a85a78dbc46516f1 EN ES PT eng spa por Pan American Health Organization https://iris.paho.org/handle/10665.2/52322 https://doaj.org/toc/1020-4989 https://doaj.org/toc/1680-5348 1020-4989 1680-5348 doi:10.26633/RPSP.2020.50 https://doaj.org/article/700da5ceb79e4b97a85a78dbc46516f1 Revista Panamericana de Salud Pública, Vol 44, Iss 50, Pp 1-7 (2020) klebsiella pneumoniae carbapenem-resistant enterobacteriaceae molecular typing venezuela Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.26633/RPSP.2020.50 2022-12-31T03:28:14Z Objective. To characterize carbapenemase-producing Klebsiella pneumoniae isolated from patients treated at a hospital in Cumaná, Sucre, Venezuela. Methods. This was a retrospective study conducted at the general hospital in Cumaná where 58 K. pneumoniae strains were analyzed for resistance to antimicrobials, specifically carbapenems, in January – June 2015. Production of metallo-β-lactamases and serine carbapenemases was determined by the double-disc synergy test, using EDTA-sodium mercaptoacetic acid and 3-aminophenyl boronic acid discs, respectively. Multiplex-PCR was used to detect genes coding for carbapenemases. Molecular typing using ERIC-PCR determined the presence of clones. Results. Four strains of K. pneumoniae resistant to carbapenems were identified. Phenotypic methods for detection of metallo-β-lactamases and serine carbapenemases were positive, and PCR demonstrated the co-presence of blaNDM and blaKPC genes in all four strains. ERIC-PCR identified two clones circulating in the hospital. Conclusions. Infection control strategies are needed at the central hospital in Cumaná and its surrounding areas to prevent the spread of these pathogens, especially given the high levels of migration from Venezuela to other countries in South America. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista Panamericana de Salud Pública 44 1 |
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klebsiella pneumoniae carbapenem-resistant enterobacteriaceae molecular typing venezuela Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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klebsiella pneumoniae carbapenem-resistant enterobacteriaceae molecular typing venezuela Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Dianny Martínez Luisa Caña Hectorina Rodulfo José García Diorelis González Lucy Rodríguez Marcos De Donato Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study |
topic_facet |
klebsiella pneumoniae carbapenem-resistant enterobacteriaceae molecular typing venezuela Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Objective. To characterize carbapenemase-producing Klebsiella pneumoniae isolated from patients treated at a hospital in Cumaná, Sucre, Venezuela. Methods. This was a retrospective study conducted at the general hospital in Cumaná where 58 K. pneumoniae strains were analyzed for resistance to antimicrobials, specifically carbapenems, in January – June 2015. Production of metallo-β-lactamases and serine carbapenemases was determined by the double-disc synergy test, using EDTA-sodium mercaptoacetic acid and 3-aminophenyl boronic acid discs, respectively. Multiplex-PCR was used to detect genes coding for carbapenemases. Molecular typing using ERIC-PCR determined the presence of clones. Results. Four strains of K. pneumoniae resistant to carbapenems were identified. Phenotypic methods for detection of metallo-β-lactamases and serine carbapenemases were positive, and PCR demonstrated the co-presence of blaNDM and blaKPC genes in all four strains. ERIC-PCR identified two clones circulating in the hospital. Conclusions. Infection control strategies are needed at the central hospital in Cumaná and its surrounding areas to prevent the spread of these pathogens, especially given the high levels of migration from Venezuela to other countries in South America. |
format |
Article in Journal/Newspaper |
author |
Dianny Martínez Luisa Caña Hectorina Rodulfo José García Diorelis González Lucy Rodríguez Marcos De Donato |
author_facet |
Dianny Martínez Luisa Caña Hectorina Rodulfo José García Diorelis González Lucy Rodríguez Marcos De Donato |
author_sort |
Dianny Martínez |
title |
Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study |
title_short |
Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study |
title_full |
Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study |
title_fullStr |
Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study |
title_full_unstemmed |
Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study |
title_sort |
characteristics of dual carbapenemase-producing klebsiella pneumoniae strains from an outbreak in venezuela: a retrospective study |
publisher |
Pan American Health Organization |
publishDate |
2020 |
url |
https://doi.org/10.26633/RPSP.2020.50 https://doaj.org/article/700da5ceb79e4b97a85a78dbc46516f1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista Panamericana de Salud Pública, Vol 44, Iss 50, Pp 1-7 (2020) |
op_relation |
https://iris.paho.org/handle/10665.2/52322 https://doaj.org/toc/1020-4989 https://doaj.org/toc/1680-5348 1020-4989 1680-5348 doi:10.26633/RPSP.2020.50 https://doaj.org/article/700da5ceb79e4b97a85a78dbc46516f1 |
op_doi |
https://doi.org/10.26633/RPSP.2020.50 |
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Revista Panamericana de Salud Pública |
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44 |
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