Green synthesis of isopropyl palmitate using immobilized Candida antarctica lipase: Process optimization using response surface methodology
This work aims to produce isopropyl palmitate (IPP), a common emollient ester in a solvent-free system. An esterification reaction between isopropyl alcohol (IPA) and palmitic acid (PA) was performed in a closed batch reactor using immobilized Candida antarctica lipase as a biocatalyst. Reaction con...
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ftdoajarticles:oai:doaj.org/article:6feb6092aa4c4afa9162c73fa62b3fa2 2023-05-15T13:33:31+02:00 Green synthesis of isopropyl palmitate using immobilized Candida antarctica lipase: Process optimization using response surface methodology Ahmad Mustafa Fumiya Niikura 2022-06-01T00:00:00Z https://doi.org/10.1016/j.clet.2022.100516 https://doaj.org/article/6feb6092aa4c4afa9162c73fa62b3fa2 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2666790822001215 https://doaj.org/toc/2666-7908 2666-7908 doi:10.1016/j.clet.2022.100516 https://doaj.org/article/6feb6092aa4c4afa9162c73fa62b3fa2 Cleaner Engineering and Technology, Vol 8, Iss , Pp 100516- (2022) Isopropyl palmitate Novozym 435 Environment Energy saving Esterification Renewable energy sources TJ807-830 Environmental engineering TA170-171 article 2022 ftdoajarticles https://doi.org/10.1016/j.clet.2022.100516 2022-12-30T21:26:05Z This work aims to produce isopropyl palmitate (IPP), a common emollient ester in a solvent-free system. An esterification reaction between isopropyl alcohol (IPA) and palmitic acid (PA) was performed in a closed batch reactor using immobilized Candida antarctica lipase as a biocatalyst. Reaction conditions were optimized using response surface methodology based on a five-level, three-variable composite design. The interactive effects of conditions on the IPP yield were investigated in the following ranges: IPA-to-PA molar ratio of 3:1–15:1, 1%–4% (w/w) Novozym 435, and 1%–10% (w/w) molecular sieves. The optimum conditions were IPA-to-PA molar ratio of 15:1, 4% w/w of Novozym 435, and 10% w/w of molecular sieves at 60°C and 150 RPM for 2.5 h. The maximum experimental and predicted conversion values were 90.00% and 90.92%, respectively. Moreover, Novozym 435 exhibited remarkable operational stability because it was used for 15 cycles without considerably losing its original activity. In studying the feasibility of the proposed method, a process flow diagram was suggested to perform the semicontinuous production of IPP in a solvent-free medium. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Cleaner Engineering and Technology 8 100516 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Isopropyl palmitate Novozym 435 Environment Energy saving Esterification Renewable energy sources TJ807-830 Environmental engineering TA170-171 |
spellingShingle |
Isopropyl palmitate Novozym 435 Environment Energy saving Esterification Renewable energy sources TJ807-830 Environmental engineering TA170-171 Ahmad Mustafa Fumiya Niikura Green synthesis of isopropyl palmitate using immobilized Candida antarctica lipase: Process optimization using response surface methodology |
topic_facet |
Isopropyl palmitate Novozym 435 Environment Energy saving Esterification Renewable energy sources TJ807-830 Environmental engineering TA170-171 |
description |
This work aims to produce isopropyl palmitate (IPP), a common emollient ester in a solvent-free system. An esterification reaction between isopropyl alcohol (IPA) and palmitic acid (PA) was performed in a closed batch reactor using immobilized Candida antarctica lipase as a biocatalyst. Reaction conditions were optimized using response surface methodology based on a five-level, three-variable composite design. The interactive effects of conditions on the IPP yield were investigated in the following ranges: IPA-to-PA molar ratio of 3:1–15:1, 1%–4% (w/w) Novozym 435, and 1%–10% (w/w) molecular sieves. The optimum conditions were IPA-to-PA molar ratio of 15:1, 4% w/w of Novozym 435, and 10% w/w of molecular sieves at 60°C and 150 RPM for 2.5 h. The maximum experimental and predicted conversion values were 90.00% and 90.92%, respectively. Moreover, Novozym 435 exhibited remarkable operational stability because it was used for 15 cycles without considerably losing its original activity. In studying the feasibility of the proposed method, a process flow diagram was suggested to perform the semicontinuous production of IPP in a solvent-free medium. |
format |
Article in Journal/Newspaper |
author |
Ahmad Mustafa Fumiya Niikura |
author_facet |
Ahmad Mustafa Fumiya Niikura |
author_sort |
Ahmad Mustafa |
title |
Green synthesis of isopropyl palmitate using immobilized Candida antarctica lipase: Process optimization using response surface methodology |
title_short |
Green synthesis of isopropyl palmitate using immobilized Candida antarctica lipase: Process optimization using response surface methodology |
title_full |
Green synthesis of isopropyl palmitate using immobilized Candida antarctica lipase: Process optimization using response surface methodology |
title_fullStr |
Green synthesis of isopropyl palmitate using immobilized Candida antarctica lipase: Process optimization using response surface methodology |
title_full_unstemmed |
Green synthesis of isopropyl palmitate using immobilized Candida antarctica lipase: Process optimization using response surface methodology |
title_sort |
green synthesis of isopropyl palmitate using immobilized candida antarctica lipase: process optimization using response surface methodology |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://doi.org/10.1016/j.clet.2022.100516 https://doaj.org/article/6feb6092aa4c4afa9162c73fa62b3fa2 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Cleaner Engineering and Technology, Vol 8, Iss , Pp 100516- (2022) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2666790822001215 https://doaj.org/toc/2666-7908 2666-7908 doi:10.1016/j.clet.2022.100516 https://doaj.org/article/6feb6092aa4c4afa9162c73fa62b3fa2 |
op_doi |
https://doi.org/10.1016/j.clet.2022.100516 |
container_title |
Cleaner Engineering and Technology |
container_volume |
8 |
container_start_page |
100516 |
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1766043122464194560 |