Comparative transcriptome profiling of virulent and avirulent isolates of Neoparamoeba perurans
Abstract Neoparamoeba perurans, the aetiological agent of amoebic gill disease, remains a persistent threat to Atlantic salmon mariculture operations worldwide. Innovation in methods of AGD control is required yet constrained by a limited understanding of the mechanisms of amoebic gill disease patho...
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ftdoajarticles:oai:doaj.org/article:6fea9f1fd31243a0886ab2fd1e90eda9 2023-05-15T15:32:23+02:00 Comparative transcriptome profiling of virulent and avirulent isolates of Neoparamoeba perurans Aaron J. Smith Philip B. B. Crosbie Barbara F. Nowak Andrew R. Bridle 2022-04-01T00:00:00Z https://doi.org/10.1038/s41598-022-09806-5 https://doaj.org/article/6fea9f1fd31243a0886ab2fd1e90eda9 EN eng Nature Portfolio https://doi.org/10.1038/s41598-022-09806-5 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-09806-5 2045-2322 https://doaj.org/article/6fea9f1fd31243a0886ab2fd1e90eda9 Scientific Reports, Vol 12, Iss 1, Pp 1-11 (2022) Medicine R Science Q article 2022 ftdoajarticles https://doi.org/10.1038/s41598-022-09806-5 2022-12-31T12:12:27Z Abstract Neoparamoeba perurans, the aetiological agent of amoebic gill disease, remains a persistent threat to Atlantic salmon mariculture operations worldwide. Innovation in methods of AGD control is required yet constrained by a limited understanding of the mechanisms of amoebic gill disease pathogenesis. In the current study, a comparative transcriptome analysis of two N. perurans isolates of contrasting virulence phenotypes is presented using gill-associated, virulent (wild type) isolates, and in vitro cultured, avirulent (clonal) isolates. Differential gene expression analysis identified a total of 21,198 differentially expressed genes between the wild type and clonal isolates, with 5674 of these genes upregulated in wild type N. perurans. Gene set enrichment analysis predicted gene sets enriched in the wild type isolates including, although not limited to, cortical actin cytoskeleton, pseudopodia, phagocytosis, macropinocytic cup, and fatty acid beta-oxidation. Combined, the results from these analyses suggest that upregulated gene expression associated with lipid metabolism, oxidative stress response, protease activity, and cytoskeleton reorganisation is linked to pathogenicity in wild type N. perurans. These findings provide a foundation for future AGD research and the development of novel therapeutic and prophylactic AGD control measures for commercial aquaculture. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Scientific Reports 12 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Medicine R Science Q |
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Medicine R Science Q Aaron J. Smith Philip B. B. Crosbie Barbara F. Nowak Andrew R. Bridle Comparative transcriptome profiling of virulent and avirulent isolates of Neoparamoeba perurans |
topic_facet |
Medicine R Science Q |
description |
Abstract Neoparamoeba perurans, the aetiological agent of amoebic gill disease, remains a persistent threat to Atlantic salmon mariculture operations worldwide. Innovation in methods of AGD control is required yet constrained by a limited understanding of the mechanisms of amoebic gill disease pathogenesis. In the current study, a comparative transcriptome analysis of two N. perurans isolates of contrasting virulence phenotypes is presented using gill-associated, virulent (wild type) isolates, and in vitro cultured, avirulent (clonal) isolates. Differential gene expression analysis identified a total of 21,198 differentially expressed genes between the wild type and clonal isolates, with 5674 of these genes upregulated in wild type N. perurans. Gene set enrichment analysis predicted gene sets enriched in the wild type isolates including, although not limited to, cortical actin cytoskeleton, pseudopodia, phagocytosis, macropinocytic cup, and fatty acid beta-oxidation. Combined, the results from these analyses suggest that upregulated gene expression associated with lipid metabolism, oxidative stress response, protease activity, and cytoskeleton reorganisation is linked to pathogenicity in wild type N. perurans. These findings provide a foundation for future AGD research and the development of novel therapeutic and prophylactic AGD control measures for commercial aquaculture. |
format |
Article in Journal/Newspaper |
author |
Aaron J. Smith Philip B. B. Crosbie Barbara F. Nowak Andrew R. Bridle |
author_facet |
Aaron J. Smith Philip B. B. Crosbie Barbara F. Nowak Andrew R. Bridle |
author_sort |
Aaron J. Smith |
title |
Comparative transcriptome profiling of virulent and avirulent isolates of Neoparamoeba perurans |
title_short |
Comparative transcriptome profiling of virulent and avirulent isolates of Neoparamoeba perurans |
title_full |
Comparative transcriptome profiling of virulent and avirulent isolates of Neoparamoeba perurans |
title_fullStr |
Comparative transcriptome profiling of virulent and avirulent isolates of Neoparamoeba perurans |
title_full_unstemmed |
Comparative transcriptome profiling of virulent and avirulent isolates of Neoparamoeba perurans |
title_sort |
comparative transcriptome profiling of virulent and avirulent isolates of neoparamoeba perurans |
publisher |
Nature Portfolio |
publishDate |
2022 |
url |
https://doi.org/10.1038/s41598-022-09806-5 https://doaj.org/article/6fea9f1fd31243a0886ab2fd1e90eda9 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Scientific Reports, Vol 12, Iss 1, Pp 1-11 (2022) |
op_relation |
https://doi.org/10.1038/s41598-022-09806-5 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-09806-5 2045-2322 https://doaj.org/article/6fea9f1fd31243a0886ab2fd1e90eda9 |
op_doi |
https://doi.org/10.1038/s41598-022-09806-5 |
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Scientific Reports |
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12 |
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1 |
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1766362888997437440 |