Whole-genome sequencing and phylogenetic analysis of rabies viruses from Jordan.
Human fatalities caused by rabies are rarely reported in Jordan; however, domestic animals are more likely to fall victim to rabies compared to wild animals, at least this is the case in Jordan due to the presence of canine rabies. In this study, twelve brain samples from domestic and wild animals s...
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ftdoajarticles:oai:doaj.org/article:6fd311585c4d4842a006087351862b26 2023-05-15T15:09:46+02:00 Whole-genome sequencing and phylogenetic analysis of rabies viruses from Jordan. Laith N Al-Eitan Guanghui Wu Megan Golding Yue Tang Hooman Goharriz Denise A Marston Anthony R Fooks Lorraine M McElhinney 2021-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009431 https://doaj.org/article/6fd311585c4d4842a006087351862b26 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009431 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009431 https://doaj.org/article/6fd311585c4d4842a006087351862b26 PLoS Neglected Tropical Diseases, Vol 15, Iss 5, p e0009431 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009431 2022-12-31T07:51:21Z Human fatalities caused by rabies are rarely reported in Jordan; however, domestic animals are more likely to fall victim to rabies compared to wild animals, at least this is the case in Jordan due to the presence of canine rabies. In this study, twelve brain samples from domestic and wild animals suspected of being infected with rabies virus from different regions of Jordan were collected during 2019. Seven of them tested positive using the fluorescent antibody test and real-time SYBR RT-PCR assay. Five specimens were from stray dogs and two from foxes. The whole genome sequences were obtained from the positive samples. Sequence analysis showed that one dog virus from Al Quwaysimah city located in Amman governorate, was closely related to an Israeli strain belonging to a Cosmopolitan ME1a clade. The genomes of the remaining six viruses (four from dogs and two from foxes) collected from different areas of Jordan were genetically-related to each other and clustered together with sequences from Iran and Turkey; all belong to Cosmopolitan ME2 clade. These sequences were analyzed with six other Jordanian rabies virus nucleoprotein (N) gene sequences available in the public database, five of them belong to ME1a clade and one belongs to ME1b clade. Rabies virus whole genome data is scarce across the Middle East. This study provides a better understanding of the molecular epidemiology of rabies virus in the region. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 5 e0009431 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Laith N Al-Eitan Guanghui Wu Megan Golding Yue Tang Hooman Goharriz Denise A Marston Anthony R Fooks Lorraine M McElhinney Whole-genome sequencing and phylogenetic analysis of rabies viruses from Jordan. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Human fatalities caused by rabies are rarely reported in Jordan; however, domestic animals are more likely to fall victim to rabies compared to wild animals, at least this is the case in Jordan due to the presence of canine rabies. In this study, twelve brain samples from domestic and wild animals suspected of being infected with rabies virus from different regions of Jordan were collected during 2019. Seven of them tested positive using the fluorescent antibody test and real-time SYBR RT-PCR assay. Five specimens were from stray dogs and two from foxes. The whole genome sequences were obtained from the positive samples. Sequence analysis showed that one dog virus from Al Quwaysimah city located in Amman governorate, was closely related to an Israeli strain belonging to a Cosmopolitan ME1a clade. The genomes of the remaining six viruses (four from dogs and two from foxes) collected from different areas of Jordan were genetically-related to each other and clustered together with sequences from Iran and Turkey; all belong to Cosmopolitan ME2 clade. These sequences were analyzed with six other Jordanian rabies virus nucleoprotein (N) gene sequences available in the public database, five of them belong to ME1a clade and one belongs to ME1b clade. Rabies virus whole genome data is scarce across the Middle East. This study provides a better understanding of the molecular epidemiology of rabies virus in the region. |
format |
Article in Journal/Newspaper |
author |
Laith N Al-Eitan Guanghui Wu Megan Golding Yue Tang Hooman Goharriz Denise A Marston Anthony R Fooks Lorraine M McElhinney |
author_facet |
Laith N Al-Eitan Guanghui Wu Megan Golding Yue Tang Hooman Goharriz Denise A Marston Anthony R Fooks Lorraine M McElhinney |
author_sort |
Laith N Al-Eitan |
title |
Whole-genome sequencing and phylogenetic analysis of rabies viruses from Jordan. |
title_short |
Whole-genome sequencing and phylogenetic analysis of rabies viruses from Jordan. |
title_full |
Whole-genome sequencing and phylogenetic analysis of rabies viruses from Jordan. |
title_fullStr |
Whole-genome sequencing and phylogenetic analysis of rabies viruses from Jordan. |
title_full_unstemmed |
Whole-genome sequencing and phylogenetic analysis of rabies viruses from Jordan. |
title_sort |
whole-genome sequencing and phylogenetic analysis of rabies viruses from jordan. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009431 https://doaj.org/article/6fd311585c4d4842a006087351862b26 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 5, p e0009431 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009431 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009431 https://doaj.org/article/6fd311585c4d4842a006087351862b26 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009431 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
5 |
container_start_page |
e0009431 |
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1766340894068310016 |