A novel totivirus and piscine reovirus (PRV) in Atlantic salmon ( Salmo salar ) with cardiomyopathy syndrome (CMS)
Abstract Background Cardiomyopathy syndrome (CMS) is a severe disease affecting large farmed Atlantic salmon. Mortality often appears without prior clinical signs, typically shortly prior to slaughter. We recently reported the finding and the complete genomic sequence of a novel piscine reovirus (PR...
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ftdoajarticles:oai:doaj.org/article:6fb830f592e344b7b51182b0c67132bf 2023-05-15T15:30:08+02:00 A novel totivirus and piscine reovirus (PRV) in Atlantic salmon ( Salmo salar ) with cardiomyopathy syndrome (CMS) Nederbragt Alexander J Pedamallu Chandra S Jung Joonil Poppe Trygve Faller Randi Kristoffersen Anja B Wiik-Nielsen Christer R Grove Søren Wiik-Nielsen Jannicke Løvoll Marie Meyerson Matthew Rimstad Espen Tengs Torstein 2010-11-01T00:00:00Z https://doi.org/10.1186/1743-422X-7-309 https://doaj.org/article/6fb830f592e344b7b51182b0c67132bf EN eng BMC http://www.virologyj.com/content/7/1/309 https://doaj.org/toc/1743-422X doi:10.1186/1743-422X-7-309 1743-422X https://doaj.org/article/6fb830f592e344b7b51182b0c67132bf Virology Journal, Vol 7, Iss 1, p 309 (2010) Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1743-422X-7-309 2022-12-31T09:08:25Z Abstract Background Cardiomyopathy syndrome (CMS) is a severe disease affecting large farmed Atlantic salmon. Mortality often appears without prior clinical signs, typically shortly prior to slaughter. We recently reported the finding and the complete genomic sequence of a novel piscine reovirus (PRV), which is associated with another cardiac disease in Atlantic salmon; heart and skeletal muscle inflammation (HSMI). In the present work we have studied whether PRV or other infectious agents may be involved in the etiology of CMS. Results Using high throughput sequencing on heart samples from natural outbreaks of CMS and from fish experimentally challenged with material from fish diagnosed with CMS a high number of sequence reads identical to the PRV genome were identified. In addition, a sequence contig from a novel totivirus could also be constructed. Using RT-qPCR, levels of PRV in tissue samples were quantified and the totivirus was detected in all samples tested from CMS fish but not in controls. In situ hybridization supported this pattern indicating a possible association between CMS and the novel piscine totivirus. Conclusions Although causality for CMS in Atlantic salmon could not be proven for either of the two viruses, our results are compatible with a hypothesis where, in the experimental challenge studied, PRV behaves as an opportunist whereas the totivirus might be more directly linked with the development of CMS. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Slaughter ENVELOPE(-85.633,-85.633,-78.617,-78.617) Virology Journal 7 1 309 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Infectious and parasitic diseases RC109-216 |
spellingShingle |
Infectious and parasitic diseases RC109-216 Nederbragt Alexander J Pedamallu Chandra S Jung Joonil Poppe Trygve Faller Randi Kristoffersen Anja B Wiik-Nielsen Christer R Grove Søren Wiik-Nielsen Jannicke Løvoll Marie Meyerson Matthew Rimstad Espen Tengs Torstein A novel totivirus and piscine reovirus (PRV) in Atlantic salmon ( Salmo salar ) with cardiomyopathy syndrome (CMS) |
topic_facet |
Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Cardiomyopathy syndrome (CMS) is a severe disease affecting large farmed Atlantic salmon. Mortality often appears without prior clinical signs, typically shortly prior to slaughter. We recently reported the finding and the complete genomic sequence of a novel piscine reovirus (PRV), which is associated with another cardiac disease in Atlantic salmon; heart and skeletal muscle inflammation (HSMI). In the present work we have studied whether PRV or other infectious agents may be involved in the etiology of CMS. Results Using high throughput sequencing on heart samples from natural outbreaks of CMS and from fish experimentally challenged with material from fish diagnosed with CMS a high number of sequence reads identical to the PRV genome were identified. In addition, a sequence contig from a novel totivirus could also be constructed. Using RT-qPCR, levels of PRV in tissue samples were quantified and the totivirus was detected in all samples tested from CMS fish but not in controls. In situ hybridization supported this pattern indicating a possible association between CMS and the novel piscine totivirus. Conclusions Although causality for CMS in Atlantic salmon could not be proven for either of the two viruses, our results are compatible with a hypothesis where, in the experimental challenge studied, PRV behaves as an opportunist whereas the totivirus might be more directly linked with the development of CMS. |
format |
Article in Journal/Newspaper |
author |
Nederbragt Alexander J Pedamallu Chandra S Jung Joonil Poppe Trygve Faller Randi Kristoffersen Anja B Wiik-Nielsen Christer R Grove Søren Wiik-Nielsen Jannicke Løvoll Marie Meyerson Matthew Rimstad Espen Tengs Torstein |
author_facet |
Nederbragt Alexander J Pedamallu Chandra S Jung Joonil Poppe Trygve Faller Randi Kristoffersen Anja B Wiik-Nielsen Christer R Grove Søren Wiik-Nielsen Jannicke Løvoll Marie Meyerson Matthew Rimstad Espen Tengs Torstein |
author_sort |
Nederbragt Alexander J |
title |
A novel totivirus and piscine reovirus (PRV) in Atlantic salmon ( Salmo salar ) with cardiomyopathy syndrome (CMS) |
title_short |
A novel totivirus and piscine reovirus (PRV) in Atlantic salmon ( Salmo salar ) with cardiomyopathy syndrome (CMS) |
title_full |
A novel totivirus and piscine reovirus (PRV) in Atlantic salmon ( Salmo salar ) with cardiomyopathy syndrome (CMS) |
title_fullStr |
A novel totivirus and piscine reovirus (PRV) in Atlantic salmon ( Salmo salar ) with cardiomyopathy syndrome (CMS) |
title_full_unstemmed |
A novel totivirus and piscine reovirus (PRV) in Atlantic salmon ( Salmo salar ) with cardiomyopathy syndrome (CMS) |
title_sort |
novel totivirus and piscine reovirus (prv) in atlantic salmon ( salmo salar ) with cardiomyopathy syndrome (cms) |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1743-422X-7-309 https://doaj.org/article/6fb830f592e344b7b51182b0c67132bf |
long_lat |
ENVELOPE(-85.633,-85.633,-78.617,-78.617) |
geographic |
Slaughter |
geographic_facet |
Slaughter |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Virology Journal, Vol 7, Iss 1, p 309 (2010) |
op_relation |
http://www.virologyj.com/content/7/1/309 https://doaj.org/toc/1743-422X doi:10.1186/1743-422X-7-309 1743-422X https://doaj.org/article/6fb830f592e344b7b51182b0c67132bf |
op_doi |
https://doi.org/10.1186/1743-422X-7-309 |
container_title |
Virology Journal |
container_volume |
7 |
container_issue |
1 |
container_start_page |
309 |
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1766360579720609792 |