Patients infected with Mycobacterium africanum versus Mycobacterium tuberculosis possess distinct intestinal microbiota.
BACKGROUND:Mycobacterium tuberculosis complex (MTBC), the causative agent of tuberculosis (TB), is composed of eight subspecies. TB in West Africa, in contrast to other geographical regions, is caused by Mycobacterium africanum (MAF) in addition to M. tuberculosis (MTB), with both infections present...
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ftdoajarticles:oai:doaj.org/article:6fa71d03555a4d8e9aba6169239c12d1 2023-05-15T15:11:40+02:00 Patients infected with Mycobacterium africanum versus Mycobacterium tuberculosis possess distinct intestinal microbiota. Sivaranjani Namasivayam Bassirou Diarra Seydou Diabate Yeya Dit Sadio Sarro Amadou Kone Bourahima Kone Mohamed Tolofoudie Bocar Baya Mahamane T Diakite Ousmane Kodio Keira Cohen Jane Holl Chad J Achenbach Soumya Chatterjee Robert Leo Murphy William Bishai Souleymane Diallo Alan Sher Mamoudou Maiga 2020-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008230 https://doaj.org/article/6fa71d03555a4d8e9aba6169239c12d1 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008230 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008230 https://doaj.org/article/6fa71d03555a4d8e9aba6169239c12d1 PLoS Neglected Tropical Diseases, Vol 14, Iss 5, p e0008230 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008230 2022-12-31T13:12:52Z BACKGROUND:Mycobacterium tuberculosis complex (MTBC), the causative agent of tuberculosis (TB), is composed of eight subspecies. TB in West Africa, in contrast to other geographical regions, is caused by Mycobacterium africanum (MAF) in addition to M. tuberculosis (MTB), with both infections presenting similar symptoms. Nevertheless, MAF is considered to be hypovirulent in comparison with MTB and less likely to progress to active disease. In this study, we asked whether MAF and MTB infected patients possess distinct intestinal microbiomes and characterized how these microbiota communities are affected by anti-tuberculosis therapy (ATT). Additionally, we assessed if the changes in microbiota composition following infection correlate with pathogen induced alterations in host blood-gene expression. METHODS:A longitudinal, clinical study of MAF infected, MTB infected patients assessed at diagnosis and two months after start of ATT, and healthy, endemic controls was conducted to compare compositions of the fecal microbiome as determined by 16S rRNA sequencing. A blood transcriptome analysis was also performed on a subset of subjects in each group by microarray and the results cross-compared with the same individual's microbiota composition. FINDINGS:MAF participants have distinct microbiomes compared with MTB patients, displaying decreased diversity and increases in Enterobacteriaceae with respect to healthy participants not observed in the latter patient group. Interestingly, this observed elevation in Enterobacteriaceae positively correlated with enhanced inflammatory gene expression in peripheral blood and was reversed after initiation of ATT. INTERPRETATION:Our findings indicate that MAF and MTB have distinct associations with the gut microbiome that may be reflective of the differential susceptibility of West Africans to these two co-endemic infections either as biomarkers or as a contributing determinant. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 5 e0008230 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sivaranjani Namasivayam Bassirou Diarra Seydou Diabate Yeya Dit Sadio Sarro Amadou Kone Bourahima Kone Mohamed Tolofoudie Bocar Baya Mahamane T Diakite Ousmane Kodio Keira Cohen Jane Holl Chad J Achenbach Soumya Chatterjee Robert Leo Murphy William Bishai Souleymane Diallo Alan Sher Mamoudou Maiga Patients infected with Mycobacterium africanum versus Mycobacterium tuberculosis possess distinct intestinal microbiota. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Mycobacterium tuberculosis complex (MTBC), the causative agent of tuberculosis (TB), is composed of eight subspecies. TB in West Africa, in contrast to other geographical regions, is caused by Mycobacterium africanum (MAF) in addition to M. tuberculosis (MTB), with both infections presenting similar symptoms. Nevertheless, MAF is considered to be hypovirulent in comparison with MTB and less likely to progress to active disease. In this study, we asked whether MAF and MTB infected patients possess distinct intestinal microbiomes and characterized how these microbiota communities are affected by anti-tuberculosis therapy (ATT). Additionally, we assessed if the changes in microbiota composition following infection correlate with pathogen induced alterations in host blood-gene expression. METHODS:A longitudinal, clinical study of MAF infected, MTB infected patients assessed at diagnosis and two months after start of ATT, and healthy, endemic controls was conducted to compare compositions of the fecal microbiome as determined by 16S rRNA sequencing. A blood transcriptome analysis was also performed on a subset of subjects in each group by microarray and the results cross-compared with the same individual's microbiota composition. FINDINGS:MAF participants have distinct microbiomes compared with MTB patients, displaying decreased diversity and increases in Enterobacteriaceae with respect to healthy participants not observed in the latter patient group. Interestingly, this observed elevation in Enterobacteriaceae positively correlated with enhanced inflammatory gene expression in peripheral blood and was reversed after initiation of ATT. INTERPRETATION:Our findings indicate that MAF and MTB have distinct associations with the gut microbiome that may be reflective of the differential susceptibility of West Africans to these two co-endemic infections either as biomarkers or as a contributing determinant. |
format |
Article in Journal/Newspaper |
author |
Sivaranjani Namasivayam Bassirou Diarra Seydou Diabate Yeya Dit Sadio Sarro Amadou Kone Bourahima Kone Mohamed Tolofoudie Bocar Baya Mahamane T Diakite Ousmane Kodio Keira Cohen Jane Holl Chad J Achenbach Soumya Chatterjee Robert Leo Murphy William Bishai Souleymane Diallo Alan Sher Mamoudou Maiga |
author_facet |
Sivaranjani Namasivayam Bassirou Diarra Seydou Diabate Yeya Dit Sadio Sarro Amadou Kone Bourahima Kone Mohamed Tolofoudie Bocar Baya Mahamane T Diakite Ousmane Kodio Keira Cohen Jane Holl Chad J Achenbach Soumya Chatterjee Robert Leo Murphy William Bishai Souleymane Diallo Alan Sher Mamoudou Maiga |
author_sort |
Sivaranjani Namasivayam |
title |
Patients infected with Mycobacterium africanum versus Mycobacterium tuberculosis possess distinct intestinal microbiota. |
title_short |
Patients infected with Mycobacterium africanum versus Mycobacterium tuberculosis possess distinct intestinal microbiota. |
title_full |
Patients infected with Mycobacterium africanum versus Mycobacterium tuberculosis possess distinct intestinal microbiota. |
title_fullStr |
Patients infected with Mycobacterium africanum versus Mycobacterium tuberculosis possess distinct intestinal microbiota. |
title_full_unstemmed |
Patients infected with Mycobacterium africanum versus Mycobacterium tuberculosis possess distinct intestinal microbiota. |
title_sort |
patients infected with mycobacterium africanum versus mycobacterium tuberculosis possess distinct intestinal microbiota. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008230 https://doaj.org/article/6fa71d03555a4d8e9aba6169239c12d1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 5, p e0008230 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008230 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008230 https://doaj.org/article/6fa71d03555a4d8e9aba6169239c12d1 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008230 |
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PLOS Neglected Tropical Diseases |
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14 |
container_issue |
5 |
container_start_page |
e0008230 |
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