Establishment and Characterization of a Testicular Cell Line from the Half-Smooth Tongue Sole, Cynoglossus semilaevis
Spermatogenesis within the adult testis is an excellent system for studying stem cell renewal and differentiation, which is under the control of testicular somatic cells. In order to understanding spermatogenesis in the half-smooth tongue sole ( Cynoglossus semilaevis) as a marine fish model of aqua...
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ftdoajarticles:oai:doaj.org/article:6f9b432b768e4effab3736f622f03812 2023-05-15T18:41:14+02:00 Establishment and Characterization of a Testicular Cell Line from the Half-Smooth Tongue Sole, Cynoglossus semilaevis Bo Zhang, Xianli Wang, Zhenxia Sha, Changgeng Yang, Shanshan Liu, Na Wang, Song-Lin Chen 2011-01-01T00:00:00Z https://doaj.org/article/6f9b432b768e4effab3736f622f03812 EN eng Ivyspring International Publisher http://www.biolsci.org/v07p0452.htm https://doaj.org/toc/1449-2288 1449-2288 https://doaj.org/article/6f9b432b768e4effab3736f622f03812 International Journal of Biological Sciences, Vol 7, Iss 4, Pp 452-459 (2011) Biology (General) QH301-705.5 article 2011 ftdoajarticles 2022-12-31T09:46:32Z Spermatogenesis within the adult testis is an excellent system for studying stem cell renewal and differentiation, which is under the control of testicular somatic cells. In order to understanding spermatogenesis in the half-smooth tongue sole ( Cynoglossus semilaevis) as a marine fish model of aquaculture importance, we established a cell line called CSGC from a juvenile gonad of this organism. CSGC is composed of fibroblast-like cells, retains a diploid karyotype of 42 chromosomes, lacks the heterogametic W chromosome, lacks a female specific marker and expresses the dmrt , a marker for testicular somatic cells. Therefore, CSGC appears to consist of testicular somatic cell cells. We show that this cell line is effective for infection by the turbot reddish body iridovirus and flounder lymphocystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electon microscopy, demonstrateing that CSGC is suitable to study interactions between virus and host cells. As a first fish testicular somatic cell line of the ZZ-ZW genetic sex determination system, CSGC will be a useful tool to study sex-related events and interactions between somatic cells and germ cells during spermatogenesis. Article in Journal/Newspaper Turbot Directory of Open Access Journals: DOAJ Articles |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Biology (General) QH301-705.5 |
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Biology (General) QH301-705.5 Bo Zhang, Xianli Wang, Zhenxia Sha, Changgeng Yang, Shanshan Liu, Na Wang, Song-Lin Chen Establishment and Characterization of a Testicular Cell Line from the Half-Smooth Tongue Sole, Cynoglossus semilaevis |
topic_facet |
Biology (General) QH301-705.5 |
description |
Spermatogenesis within the adult testis is an excellent system for studying stem cell renewal and differentiation, which is under the control of testicular somatic cells. In order to understanding spermatogenesis in the half-smooth tongue sole ( Cynoglossus semilaevis) as a marine fish model of aquaculture importance, we established a cell line called CSGC from a juvenile gonad of this organism. CSGC is composed of fibroblast-like cells, retains a diploid karyotype of 42 chromosomes, lacks the heterogametic W chromosome, lacks a female specific marker and expresses the dmrt , a marker for testicular somatic cells. Therefore, CSGC appears to consist of testicular somatic cell cells. We show that this cell line is effective for infection by the turbot reddish body iridovirus and flounder lymphocystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electon microscopy, demonstrateing that CSGC is suitable to study interactions between virus and host cells. As a first fish testicular somatic cell line of the ZZ-ZW genetic sex determination system, CSGC will be a useful tool to study sex-related events and interactions between somatic cells and germ cells during spermatogenesis. |
format |
Article in Journal/Newspaper |
author |
Bo Zhang, Xianli Wang, Zhenxia Sha, Changgeng Yang, Shanshan Liu, Na Wang, Song-Lin Chen |
author_facet |
Bo Zhang, Xianli Wang, Zhenxia Sha, Changgeng Yang, Shanshan Liu, Na Wang, Song-Lin Chen |
author_sort |
Bo Zhang, Xianli Wang, Zhenxia Sha, Changgeng Yang, Shanshan Liu, Na Wang, Song-Lin Chen |
title |
Establishment and Characterization of a Testicular Cell Line from the Half-Smooth Tongue Sole, Cynoglossus semilaevis |
title_short |
Establishment and Characterization of a Testicular Cell Line from the Half-Smooth Tongue Sole, Cynoglossus semilaevis |
title_full |
Establishment and Characterization of a Testicular Cell Line from the Half-Smooth Tongue Sole, Cynoglossus semilaevis |
title_fullStr |
Establishment and Characterization of a Testicular Cell Line from the Half-Smooth Tongue Sole, Cynoglossus semilaevis |
title_full_unstemmed |
Establishment and Characterization of a Testicular Cell Line from the Half-Smooth Tongue Sole, Cynoglossus semilaevis |
title_sort |
establishment and characterization of a testicular cell line from the half-smooth tongue sole, cynoglossus semilaevis |
publisher |
Ivyspring International Publisher |
publishDate |
2011 |
url |
https://doaj.org/article/6f9b432b768e4effab3736f622f03812 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
International Journal of Biological Sciences, Vol 7, Iss 4, Pp 452-459 (2011) |
op_relation |
http://www.biolsci.org/v07p0452.htm https://doaj.org/toc/1449-2288 1449-2288 https://doaj.org/article/6f9b432b768e4effab3736f622f03812 |
_version_ |
1766230729101934592 |