Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers
Abstract Background Colonization of the body is an important step in Staphylococcus aureus infection. S. aureus colonizes skin and mucous membranes in humans and several animal species. One important ecological niche of S. aureus is the anterior nares. More than 60% of the S. aureus in the nose are...
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ftdoajarticles:oai:doaj.org/article:6f9a381abcfe48808628e6f4fa9ffddf 2023-05-15T18:34:44+02:00 Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers Anne-Merethe Hanssen Bert Kindlund Niels Christian Stenklev Anne-Sofie Furberg Silje Fismen Renate Slind Olsen Mona Johannessen Johanna Ulrica Ericson Sollid 2017-04-01T00:00:00Z https://doi.org/10.1186/s12866-017-0997-3 https://doaj.org/article/6f9a381abcfe48808628e6f4fa9ffddf EN eng BMC http://link.springer.com/article/10.1186/s12866-017-0997-3 https://doaj.org/toc/1471-2180 doi:10.1186/s12866-017-0997-3 1471-2180 https://doaj.org/article/6f9a381abcfe48808628e6f4fa9ffddf BMC Microbiology, Vol 17, Iss 1, Pp 1-11 (2017) Nasal colonization Staphylococcus aureus Carrier Localization Epithelial cells Intracellular Microbiology QR1-502 article 2017 ftdoajarticles https://doi.org/10.1186/s12866-017-0997-3 2022-12-31T13:09:37Z Abstract Background Colonization of the body is an important step in Staphylococcus aureus infection. S. aureus colonizes skin and mucous membranes in humans and several animal species. One important ecological niche of S. aureus is the anterior nares. More than 60% of the S. aureus in the nose are found in vestibulum nasi. Our aim was to describe the localization of S. aureus in nasal tissue from healthy carriers. Methods Punch skin biopsies were taken from vestibulum nasi from healthy volunteers (S. aureus carriers and non−/intermittent carriers, n = 39) attending the population-based Tromsø 6 study. The tissue samples were processed as frozen sections before immunostaining with a specific S. aureus antibody, and finally evaluated by a confocal laser-scanning microscope. Results Our results suggest that S. aureus colonize both the upper and lower layers of the epidermis within the nasal epithelium of healthy individuals. The number of S. aureus in epidermis was surprisingly low. Intracellular localization of S. aureus in nasal tissue from healthy individuals was also detected. Conclusions Knowledge of the exact localization of S. aureus in nasal tissue is important for the understanding of the host responses against S. aureus. Our results may have consequences for the eradication strategy of S. aureus in carriers, and further work can provide us with tools for targeted prevention of S. aureus colonisation and infection. Article in Journal/Newspaper Tromsø Directory of Open Access Journals: DOAJ Articles Tromsø Nares ENVELOPE(158.167,158.167,-81.450,-81.450) Nasi ENVELOPE(-23.036,-23.036,66.336,66.336) BMC Microbiology 17 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Nasal colonization Staphylococcus aureus Carrier Localization Epithelial cells Intracellular Microbiology QR1-502 |
spellingShingle |
Nasal colonization Staphylococcus aureus Carrier Localization Epithelial cells Intracellular Microbiology QR1-502 Anne-Merethe Hanssen Bert Kindlund Niels Christian Stenklev Anne-Sofie Furberg Silje Fismen Renate Slind Olsen Mona Johannessen Johanna Ulrica Ericson Sollid Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
topic_facet |
Nasal colonization Staphylococcus aureus Carrier Localization Epithelial cells Intracellular Microbiology QR1-502 |
description |
Abstract Background Colonization of the body is an important step in Staphylococcus aureus infection. S. aureus colonizes skin and mucous membranes in humans and several animal species. One important ecological niche of S. aureus is the anterior nares. More than 60% of the S. aureus in the nose are found in vestibulum nasi. Our aim was to describe the localization of S. aureus in nasal tissue from healthy carriers. Methods Punch skin biopsies were taken from vestibulum nasi from healthy volunteers (S. aureus carriers and non−/intermittent carriers, n = 39) attending the population-based Tromsø 6 study. The tissue samples were processed as frozen sections before immunostaining with a specific S. aureus antibody, and finally evaluated by a confocal laser-scanning microscope. Results Our results suggest that S. aureus colonize both the upper and lower layers of the epidermis within the nasal epithelium of healthy individuals. The number of S. aureus in epidermis was surprisingly low. Intracellular localization of S. aureus in nasal tissue from healthy individuals was also detected. Conclusions Knowledge of the exact localization of S. aureus in nasal tissue is important for the understanding of the host responses against S. aureus. Our results may have consequences for the eradication strategy of S. aureus in carriers, and further work can provide us with tools for targeted prevention of S. aureus colonisation and infection. |
format |
Article in Journal/Newspaper |
author |
Anne-Merethe Hanssen Bert Kindlund Niels Christian Stenklev Anne-Sofie Furberg Silje Fismen Renate Slind Olsen Mona Johannessen Johanna Ulrica Ericson Sollid |
author_facet |
Anne-Merethe Hanssen Bert Kindlund Niels Christian Stenklev Anne-Sofie Furberg Silje Fismen Renate Slind Olsen Mona Johannessen Johanna Ulrica Ericson Sollid |
author_sort |
Anne-Merethe Hanssen |
title |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_short |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_full |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_fullStr |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_full_unstemmed |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_sort |
localization of staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12866-017-0997-3 https://doaj.org/article/6f9a381abcfe48808628e6f4fa9ffddf |
long_lat |
ENVELOPE(158.167,158.167,-81.450,-81.450) ENVELOPE(-23.036,-23.036,66.336,66.336) |
geographic |
Tromsø Nares Nasi |
geographic_facet |
Tromsø Nares Nasi |
genre |
Tromsø |
genre_facet |
Tromsø |
op_source |
BMC Microbiology, Vol 17, Iss 1, Pp 1-11 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12866-017-0997-3 https://doaj.org/toc/1471-2180 doi:10.1186/s12866-017-0997-3 1471-2180 https://doaj.org/article/6f9a381abcfe48808628e6f4fa9ffddf |
op_doi |
https://doi.org/10.1186/s12866-017-0997-3 |
container_title |
BMC Microbiology |
container_volume |
17 |
container_issue |
1 |
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1766219630760689664 |