Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase
Microorganisms present in Antarctica have to deal not only with cold temperatures but also with other environmental conditions, such as high UV radiation, that trigger the generation of reactive oxygen species. Therefore, Antarctic microorganisms must have an important antioxidant defense system to...
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ftdoajarticles:oai:doaj.org/article:6f8a90d054ca4017afc690c0bf8805b1 2023-05-15T13:46:46+02:00 Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase María T. Monsalves Gabriela P. Ollivet-Besson Maximiliano J. Amenabar Jenny M. Blamey 2020-01-01T00:00:00Z https://doi.org/10.3390/microorganisms8010095 https://doaj.org/article/6f8a90d054ca4017afc690c0bf8805b1 EN eng MDPI AG https://www.mdpi.com/2076-2607/8/1/95 https://doaj.org/toc/2076-2607 2076-2607 doi:10.3390/microorganisms8010095 https://doaj.org/article/6f8a90d054ca4017afc690c0bf8805b1 Microorganisms, Vol 8, Iss 1, p 95 (2020) antarctica serratia psychrotolerant uv-c radiation catalase thermostable antioxidant ros oxidative stress Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.3390/microorganisms8010095 2022-12-31T10:29:26Z Microorganisms present in Antarctica have to deal not only with cold temperatures but also with other environmental conditions, such as high UV radiation, that trigger the generation of reactive oxygen species. Therefore, Antarctic microorganisms must have an important antioxidant defense system to prevent oxidative damage. One of these defenses are antioxidant enzymes, such as catalase, which is involved in the detoxification of hydrogen peroxide produced under oxidative conditions. Here, we reported the isolation and partial characterization of an Antarctic bacterium belonging to the Serratia genus that was resistant to UV-C radiation and well-adapted to cold temperatures. This microorganism, denominated strain I1P, was efficient at decreasing reactive oxygen species levels produced after UV-C irradiation. Genomic and activity assays suggested that the enzymatic antioxidant defense mechanisms of strain I1P, especially its catalase enzyme, may confer UV resistance. This catalase was active in a wide range of temperatures (20−70 °C), showing optimal activity at 50 °C (at pH 7.0), a remarkable finding considering its psychrotolerant origin. In addition, this enzyme was thermostable, retaining around 60% of its activity after 6 h of incubation at 50 °C. The antioxidant defense systems of strain I1P, including its surprisingly thermoactive and thermostable catalase enzyme, make this microorganism a good source of biocompounds with potential biotechnological applications. Article in Journal/Newspaper Antarc* Antarctic Antarctica Elephant Island Directory of Open Access Journals: DOAJ Articles Antarctic Elephant Island ENVELOPE(-55.184,-55.184,-61.085,-61.085) Microorganisms 8 1 95 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
antarctica serratia psychrotolerant uv-c radiation catalase thermostable antioxidant ros oxidative stress Biology (General) QH301-705.5 |
spellingShingle |
antarctica serratia psychrotolerant uv-c radiation catalase thermostable antioxidant ros oxidative stress Biology (General) QH301-705.5 María T. Monsalves Gabriela P. Ollivet-Besson Maximiliano J. Amenabar Jenny M. Blamey Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase |
topic_facet |
antarctica serratia psychrotolerant uv-c radiation catalase thermostable antioxidant ros oxidative stress Biology (General) QH301-705.5 |
description |
Microorganisms present in Antarctica have to deal not only with cold temperatures but also with other environmental conditions, such as high UV radiation, that trigger the generation of reactive oxygen species. Therefore, Antarctic microorganisms must have an important antioxidant defense system to prevent oxidative damage. One of these defenses are antioxidant enzymes, such as catalase, which is involved in the detoxification of hydrogen peroxide produced under oxidative conditions. Here, we reported the isolation and partial characterization of an Antarctic bacterium belonging to the Serratia genus that was resistant to UV-C radiation and well-adapted to cold temperatures. This microorganism, denominated strain I1P, was efficient at decreasing reactive oxygen species levels produced after UV-C irradiation. Genomic and activity assays suggested that the enzymatic antioxidant defense mechanisms of strain I1P, especially its catalase enzyme, may confer UV resistance. This catalase was active in a wide range of temperatures (20−70 °C), showing optimal activity at 50 °C (at pH 7.0), a remarkable finding considering its psychrotolerant origin. In addition, this enzyme was thermostable, retaining around 60% of its activity after 6 h of incubation at 50 °C. The antioxidant defense systems of strain I1P, including its surprisingly thermoactive and thermostable catalase enzyme, make this microorganism a good source of biocompounds with potential biotechnological applications. |
format |
Article in Journal/Newspaper |
author |
María T. Monsalves Gabriela P. Ollivet-Besson Maximiliano J. Amenabar Jenny M. Blamey |
author_facet |
María T. Monsalves Gabriela P. Ollivet-Besson Maximiliano J. Amenabar Jenny M. Blamey |
author_sort |
María T. Monsalves |
title |
Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase |
title_short |
Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase |
title_full |
Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase |
title_fullStr |
Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase |
title_full_unstemmed |
Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase |
title_sort |
isolation of a psychrotolerant and uv-c-resistant bacterium from elephant island, antarctica with a highly thermoactive and thermostable catalase |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8010095 https://doaj.org/article/6f8a90d054ca4017afc690c0bf8805b1 |
long_lat |
ENVELOPE(-55.184,-55.184,-61.085,-61.085) |
geographic |
Antarctic Elephant Island |
geographic_facet |
Antarctic Elephant Island |
genre |
Antarc* Antarctic Antarctica Elephant Island |
genre_facet |
Antarc* Antarctic Antarctica Elephant Island |
op_source |
Microorganisms, Vol 8, Iss 1, p 95 (2020) |
op_relation |
https://www.mdpi.com/2076-2607/8/1/95 https://doaj.org/toc/2076-2607 2076-2607 doi:10.3390/microorganisms8010095 https://doaj.org/article/6f8a90d054ca4017afc690c0bf8805b1 |
op_doi |
https://doi.org/10.3390/microorganisms8010095 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
1 |
container_start_page |
95 |
_version_ |
1766245191194247168 |