Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton
Chlorophyll fluorescence-based estimates of primary productivity typically include dark or low-light pre-treatments to relax non-photochemical quenching (NPQ), a process that influences the relationship between PSII photochemistry and fluorescence yields. The time-scales of NPQ relaxation vary signi...
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ftdoajarticles:oai:doaj.org/article:6f492d62d14d432f948d1dc4c282d9f6 2024-09-15T17:54:15+00:00 Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton Yayla Sezginer Douglas Campbell Sacchinandan Pillai Philippe Tortell 2023-12-01T00:00:00Z https://doi.org/10.3389/fmicb.2023.1294521 https://doaj.org/article/6f492d62d14d432f948d1dc4c282d9f6 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1294521/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2023.1294521 https://doaj.org/article/6f492d62d14d432f948d1dc4c282d9f6 Frontiers in Microbiology, Vol 14 (2023) chlorophyll fluorescence non-photochemical quenching (NPQ) Arctic phytoplankton primary productivity photosynthetic electron transport rates Microbiology QR1-502 article 2023 ftdoajarticles https://doi.org/10.3389/fmicb.2023.1294521 2024-08-05T17:50:05Z Chlorophyll fluorescence-based estimates of primary productivity typically include dark or low-light pre-treatments to relax non-photochemical quenching (NPQ), a process that influences the relationship between PSII photochemistry and fluorescence yields. The time-scales of NPQ relaxation vary significantly between phytoplankton taxa and across environmental conditions, creating uncertainty in field-based productivity measurements derived from fluorescence. To address this practical challenge, we used fast repetition rate fluorometry to characterize NPQ relaxation kinetics in Arctic Ocean phytoplankton assemblages across a range of hydrographic regimes. Applying numerical fits to our data, we derived NPQ relaxation life times, and determined the relative contributions of various quenching components to the total NPQ signature across the different assemblages. Relaxation kinetics were best described as a combination of fast-, intermediate- and slow-relaxing processes, operating on time-scales of seconds, minutes, and hours, respectively. Across sampling locations and depths, total fluorescence quenching was dominated by the intermediate quenching component. Our results demonstrated an average NPQ relaxation life time of 20 ± 1.9 min, with faster relaxation among high light acclimated surface samples relative to lowlight acclimated sub-surface samples. We also used our results to examine the influence of NPQ relaxation on estimates of photosynthetic electron transport rates (ETR), testing the commonly held assumption that NPQ exerts proportional effects on light absorption (PSII functional absorption cross section, σPSII) and photochemical quantum efficiency (FV/FM). This assumption was violated in a number of phytoplankton assemblages that showed a significant decoupling of σPSII and FV/FM during NPQ relaxation, and an associated variability in ETR estimates. Decoupling of σPSII and FV/FM was most prevalent in samples displaying symptoms photoinhibition. Our results provide insights into the mechanisms and ... Article in Journal/Newspaper Arctic Ocean Phytoplankton Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 14 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
chlorophyll fluorescence non-photochemical quenching (NPQ) Arctic phytoplankton primary productivity photosynthetic electron transport rates Microbiology QR1-502 |
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chlorophyll fluorescence non-photochemical quenching (NPQ) Arctic phytoplankton primary productivity photosynthetic electron transport rates Microbiology QR1-502 Yayla Sezginer Douglas Campbell Sacchinandan Pillai Philippe Tortell Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton |
topic_facet |
chlorophyll fluorescence non-photochemical quenching (NPQ) Arctic phytoplankton primary productivity photosynthetic electron transport rates Microbiology QR1-502 |
description |
Chlorophyll fluorescence-based estimates of primary productivity typically include dark or low-light pre-treatments to relax non-photochemical quenching (NPQ), a process that influences the relationship between PSII photochemistry and fluorescence yields. The time-scales of NPQ relaxation vary significantly between phytoplankton taxa and across environmental conditions, creating uncertainty in field-based productivity measurements derived from fluorescence. To address this practical challenge, we used fast repetition rate fluorometry to characterize NPQ relaxation kinetics in Arctic Ocean phytoplankton assemblages across a range of hydrographic regimes. Applying numerical fits to our data, we derived NPQ relaxation life times, and determined the relative contributions of various quenching components to the total NPQ signature across the different assemblages. Relaxation kinetics were best described as a combination of fast-, intermediate- and slow-relaxing processes, operating on time-scales of seconds, minutes, and hours, respectively. Across sampling locations and depths, total fluorescence quenching was dominated by the intermediate quenching component. Our results demonstrated an average NPQ relaxation life time of 20 ± 1.9 min, with faster relaxation among high light acclimated surface samples relative to lowlight acclimated sub-surface samples. We also used our results to examine the influence of NPQ relaxation on estimates of photosynthetic electron transport rates (ETR), testing the commonly held assumption that NPQ exerts proportional effects on light absorption (PSII functional absorption cross section, σPSII) and photochemical quantum efficiency (FV/FM). This assumption was violated in a number of phytoplankton assemblages that showed a significant decoupling of σPSII and FV/FM during NPQ relaxation, and an associated variability in ETR estimates. Decoupling of σPSII and FV/FM was most prevalent in samples displaying symptoms photoinhibition. Our results provide insights into the mechanisms and ... |
format |
Article in Journal/Newspaper |
author |
Yayla Sezginer Douglas Campbell Sacchinandan Pillai Philippe Tortell |
author_facet |
Yayla Sezginer Douglas Campbell Sacchinandan Pillai Philippe Tortell |
author_sort |
Yayla Sezginer |
title |
Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton |
title_short |
Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton |
title_full |
Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton |
title_fullStr |
Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton |
title_full_unstemmed |
Fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in Arctic phytoplankton |
title_sort |
fluorescence-based primary productivity estimates are influenced by non-photochemical quenching dynamics in arctic phytoplankton |
publisher |
Frontiers Media S.A. |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmicb.2023.1294521 https://doaj.org/article/6f492d62d14d432f948d1dc4c282d9f6 |
genre |
Arctic Ocean Phytoplankton |
genre_facet |
Arctic Ocean Phytoplankton |
op_source |
Frontiers in Microbiology, Vol 14 (2023) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1294521/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2023.1294521 https://doaj.org/article/6f492d62d14d432f948d1dc4c282d9f6 |
op_doi |
https://doi.org/10.3389/fmicb.2023.1294521 |
container_title |
Frontiers in Microbiology |
container_volume |
14 |
_version_ |
1810430489593380864 |