Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus
INTRODUCTION: Antimicrobial activity on biofilms depends on their molecular size, positive charges, permeability coefficient, and bactericidal activity. Vancomycin is the primary choice for methicillin-resistant Staphylococcus aureus (MRSA) infection treatment; rifampicin has interesting antibiofilm...
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ftdoajarticles:oai:doaj.org/article:6f46dcd6b9bf4ea0a449d62adc1ccc6c 2023-05-15T15:07:27+02:00 Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus Keli Cristine Reiter Gustavo Enck Sambrano Bárbara Villa Thiago Galvão da Silva Paim Caio Fernando de Oliveira Pedro Alves d'Azevedo 2012-08-01T00:00:00Z https://doi.org/10.1590/S0037-86822012000400011 https://doaj.org/article/6f46dcd6b9bf4ea0a449d62adc1ccc6c EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400011&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000400011 https://doaj.org/article/6f46dcd6b9bf4ea0a449d62adc1ccc6c Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 4, Pp 471-474 (2012) Staphylococcus aureus Rifampicina Vancomicina Biofilme Resistência Arctic medicine. Tropical medicine RC955-962 article 2012 ftdoajarticles https://doi.org/10.1590/S0037-86822012000400011 2022-12-31T03:21:46Z INTRODUCTION: Antimicrobial activity on biofilms depends on their molecular size, positive charges, permeability coefficient, and bactericidal activity. Vancomycin is the primary choice for methicillin-resistant Staphylococcus aureus (MRSA) infection treatment; rifampicin has interesting antibiofilm properties, but its effectivity remains poorly defined. METHODS: Rifampicin activity alone and in combination with vancomycin against biofilm-forming MRSA was investigated, using a twofold serial broth microtiter method, biofilm challenge, and bacterial count recovery. RESULTS: Minimal inhibitory concentration (MIC) and minimal bactericidal concentration for vancomycin and rifampicin ranged from 0.5 to 1mg/l and 0.008 to 4mg/l, and from 1 to 4mg/l and 0.06 to 32mg/l, respectively. Mature biofilms were submitted to rifampicin and vancomycin exposure, and minimum biofilm eradication concentration ranged from 64 to 32,000 folds and from 32 to 512 folds higher than those for planktonic cells, respectively. Vancomycin (15mg/l) in combination with rifampicin at 6 dilutions higher each isolate MIC did not reach in vitro biofilm eradication but showed biofilm inhibitory capacity (1.43 and 0.56log10 CFU/ml reduction for weak and strong biofilm producers, respectively; p<0.05). CONCLUSIONS: In our setting, rifampicin alone failed to effectively kill biofilm-forming MRSA, demonstrating stronger inability to eradicate mature biofilm compared with vancomycin. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 45 4 471 474 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Staphylococcus aureus Rifampicina Vancomicina Biofilme Resistência Arctic medicine. Tropical medicine RC955-962 |
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Staphylococcus aureus Rifampicina Vancomicina Biofilme Resistência Arctic medicine. Tropical medicine RC955-962 Keli Cristine Reiter Gustavo Enck Sambrano Bárbara Villa Thiago Galvão da Silva Paim Caio Fernando de Oliveira Pedro Alves d'Azevedo Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus |
topic_facet |
Staphylococcus aureus Rifampicina Vancomicina Biofilme Resistência Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Antimicrobial activity on biofilms depends on their molecular size, positive charges, permeability coefficient, and bactericidal activity. Vancomycin is the primary choice for methicillin-resistant Staphylococcus aureus (MRSA) infection treatment; rifampicin has interesting antibiofilm properties, but its effectivity remains poorly defined. METHODS: Rifampicin activity alone and in combination with vancomycin against biofilm-forming MRSA was investigated, using a twofold serial broth microtiter method, biofilm challenge, and bacterial count recovery. RESULTS: Minimal inhibitory concentration (MIC) and minimal bactericidal concentration for vancomycin and rifampicin ranged from 0.5 to 1mg/l and 0.008 to 4mg/l, and from 1 to 4mg/l and 0.06 to 32mg/l, respectively. Mature biofilms were submitted to rifampicin and vancomycin exposure, and minimum biofilm eradication concentration ranged from 64 to 32,000 folds and from 32 to 512 folds higher than those for planktonic cells, respectively. Vancomycin (15mg/l) in combination with rifampicin at 6 dilutions higher each isolate MIC did not reach in vitro biofilm eradication but showed biofilm inhibitory capacity (1.43 and 0.56log10 CFU/ml reduction for weak and strong biofilm producers, respectively; p<0.05). CONCLUSIONS: In our setting, rifampicin alone failed to effectively kill biofilm-forming MRSA, demonstrating stronger inability to eradicate mature biofilm compared with vancomycin. |
format |
Article in Journal/Newspaper |
author |
Keli Cristine Reiter Gustavo Enck Sambrano Bárbara Villa Thiago Galvão da Silva Paim Caio Fernando de Oliveira Pedro Alves d'Azevedo |
author_facet |
Keli Cristine Reiter Gustavo Enck Sambrano Bárbara Villa Thiago Galvão da Silva Paim Caio Fernando de Oliveira Pedro Alves d'Azevedo |
author_sort |
Keli Cristine Reiter |
title |
Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus |
title_short |
Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus |
title_full |
Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus |
title_fullStr |
Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus |
title_full_unstemmed |
Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus |
title_sort |
rifampicin fails to eradicate mature biofilm formed by methicillin-resistant staphylococcus aureus |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2012 |
url |
https://doi.org/10.1590/S0037-86822012000400011 https://doaj.org/article/6f46dcd6b9bf4ea0a449d62adc1ccc6c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 4, Pp 471-474 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400011&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000400011 https://doaj.org/article/6f46dcd6b9bf4ea0a449d62adc1ccc6c |
op_doi |
https://doi.org/10.1590/S0037-86822012000400011 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
45 |
container_issue |
4 |
container_start_page |
471 |
op_container_end_page |
474 |
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1766338953167765504 |