Detection of arboviruses in mosquitoes: Evidence of circulation of chikungunya virus in Iran.
Mosquitoes are vectors of viruses affecting animal and human health. In Iran, the prevalence of mosquito-borne viruses remains poorly investigated. Once infected, mosquito females remain infected for all their life making virus detections possible at early steps before infections are reported in ver...
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ftdoajarticles:oai:doaj.org/article:6f3e327b674e4c86ba0d2ffc20079fff 2023-05-15T15:06:40+02:00 Detection of arboviruses in mosquitoes: Evidence of circulation of chikungunya virus in Iran. Hasan Bakhshi Laurence Mousson Sara Moutailler Marie Vazeille Géraldine Piorkowski Sedigheh Zakeri Abbasali Raz Xavier de Lamballerie Navid Dinparast-Djadid Anna-Bella Failloux 2020-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008135 https://doaj.org/article/6f3e327b674e4c86ba0d2ffc20079fff EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008135 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008135 https://doaj.org/article/6f3e327b674e4c86ba0d2ffc20079fff PLoS Neglected Tropical Diseases, Vol 14, Iss 6, p e0008135 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008135 2022-12-31T11:46:20Z Mosquitoes are vectors of viruses affecting animal and human health. In Iran, the prevalence of mosquito-borne viruses remains poorly investigated. Once infected, mosquito females remain infected for all their life making virus detections possible at early steps before infections are reported in vertebrate hosts. In this study, we used a recently developed high-throughput chip based on the BioMark Dynamic arrays system capable of detecting 37 arboviruses in a single experiment. A total of 1,212 mosquitoes collected in Mazandaran, North-Khorasan, and Fars provinces of Iran were analyzed. Eighteen species were identified, belonging to five genera; the most prevalent species were Anopheles maculipennis s.l. (42.41%), Culex pipiens (19.39%), An. superpictus (11.72%), and Cx. tritaeniorhynchus (10.64%). We detected chikungunya virus (CHIKV) of the Asian genotype in six mosquito pools collected in North Khorasan and Mazandaran provinces. To our knowledge, this is the first report of mosquitoes infected with CHIKV in Iran. Our high-throughput screening method can be proposed as a novel epidemiological surveillance tool to identify circulating arboviruses and to support preparedness to an epidemic in animals and humans. Article in Journal/Newspaper Arctic Human health Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 6 e0008135 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Hasan Bakhshi Laurence Mousson Sara Moutailler Marie Vazeille Géraldine Piorkowski Sedigheh Zakeri Abbasali Raz Xavier de Lamballerie Navid Dinparast-Djadid Anna-Bella Failloux Detection of arboviruses in mosquitoes: Evidence of circulation of chikungunya virus in Iran. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Mosquitoes are vectors of viruses affecting animal and human health. In Iran, the prevalence of mosquito-borne viruses remains poorly investigated. Once infected, mosquito females remain infected for all their life making virus detections possible at early steps before infections are reported in vertebrate hosts. In this study, we used a recently developed high-throughput chip based on the BioMark Dynamic arrays system capable of detecting 37 arboviruses in a single experiment. A total of 1,212 mosquitoes collected in Mazandaran, North-Khorasan, and Fars provinces of Iran were analyzed. Eighteen species were identified, belonging to five genera; the most prevalent species were Anopheles maculipennis s.l. (42.41%), Culex pipiens (19.39%), An. superpictus (11.72%), and Cx. tritaeniorhynchus (10.64%). We detected chikungunya virus (CHIKV) of the Asian genotype in six mosquito pools collected in North Khorasan and Mazandaran provinces. To our knowledge, this is the first report of mosquitoes infected with CHIKV in Iran. Our high-throughput screening method can be proposed as a novel epidemiological surveillance tool to identify circulating arboviruses and to support preparedness to an epidemic in animals and humans. |
format |
Article in Journal/Newspaper |
author |
Hasan Bakhshi Laurence Mousson Sara Moutailler Marie Vazeille Géraldine Piorkowski Sedigheh Zakeri Abbasali Raz Xavier de Lamballerie Navid Dinparast-Djadid Anna-Bella Failloux |
author_facet |
Hasan Bakhshi Laurence Mousson Sara Moutailler Marie Vazeille Géraldine Piorkowski Sedigheh Zakeri Abbasali Raz Xavier de Lamballerie Navid Dinparast-Djadid Anna-Bella Failloux |
author_sort |
Hasan Bakhshi |
title |
Detection of arboviruses in mosquitoes: Evidence of circulation of chikungunya virus in Iran. |
title_short |
Detection of arboviruses in mosquitoes: Evidence of circulation of chikungunya virus in Iran. |
title_full |
Detection of arboviruses in mosquitoes: Evidence of circulation of chikungunya virus in Iran. |
title_fullStr |
Detection of arboviruses in mosquitoes: Evidence of circulation of chikungunya virus in Iran. |
title_full_unstemmed |
Detection of arboviruses in mosquitoes: Evidence of circulation of chikungunya virus in Iran. |
title_sort |
detection of arboviruses in mosquitoes: evidence of circulation of chikungunya virus in iran. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008135 https://doaj.org/article/6f3e327b674e4c86ba0d2ffc20079fff |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Human health |
genre_facet |
Arctic Human health |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 6, p e0008135 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008135 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008135 https://doaj.org/article/6f3e327b674e4c86ba0d2ffc20079fff |
op_doi |
https://doi.org/10.1371/journal.pntd.0008135 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
6 |
container_start_page |
e0008135 |
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1766338227664322560 |