Characteristics of hemolytic activity induced by the aqueous extract of the Mexican fire coral Millepora complanata
BackgroundMillepora complanata is a plate-like fire coral common throughout the Caribbean. Contact with this species usually provokes burning pain, erythema and urticariform lesions. Our previous study suggested that the aqueous extract of M. complanata contains non-protein hemolysins that are solub...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:6f2f8f81f8f84e9db492035c4f163a3e 2023-05-15T15:14:46+02:00 Characteristics of hemolytic activity induced by the aqueous extract of the Mexican fire coral Millepora complanata Alejandro García-Arredondo Luis J Murillo-Esquivel Alejandra Rojas Judith Sanchez-Rodriguez 2014-12-01T00:00:00Z https://doi.org/10.1186/1678-9199-20-49 https://doaj.org/article/6f2f8f81f8f84e9db492035c4f163a3e EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200339&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-20-49 https://doaj.org/article/6f2f8f81f8f84e9db492035c4f163a3e Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 20, Iss 0 (2014) Cytolysins Hemolysins Fire coral Millepora complanata Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2014 ftdoajarticles https://doi.org/10.1186/1678-9199-20-49 2022-12-31T14:37:55Z BackgroundMillepora complanata is a plate-like fire coral common throughout the Caribbean. Contact with this species usually provokes burning pain, erythema and urticariform lesions. Our previous study suggested that the aqueous extract of M. complanata contains non-protein hemolysins that are soluble in water and ethanol. In general, the local damage induced by cnidarian venoms has been associated with hemolysins. The characterization of the effects of these components is important for the understanding of the defense mechanisms of fire corals. In addition, this information could lead to better care for victims of envenomation accidents.Methods An ethanolic extract from the lyophilized aqueous extract was prepared and its hemolytic activity was compared with the hemolysis induced by the denatured aqueous extract. Based on the finding that ethanol failed to induce nematocyst discharge, ethanolic extracts were prepared from artificially bleached and normal M. complanata fragments and their hemolytic activity was tested in order to obtain information about the source of the heat-stable hemolysins.Results Rodent erythrocytes were more susceptible to the aqueous extract than chicken and human erythrocytes. Hemolytic activity started at ten minutes of incubation and was relatively stable within the range of 28-50°C. When the aqueous extract was preincubated at temperatures over 60°C, hemolytic activity was significantly reduced. The denatured extract induced a slow hemolytic activity (HU50= 1,050.00 ± 45.85 μg/mL), detectable four hours after incubation, which was similar to that induced by the ethanolic extract prepared from the aqueous extract (HU50= 1,167.00 ± 54.95 μg/mL). No significant differences were observed between hemolysis induced by ethanolic extracts from bleached and normal fragments, although both activities were more potent than hemolysis induced by the denatured extract.Conclusions The results showed that the aqueous extract of M. complanata possesses one or more powerful heat-labile hemolytic ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 20 1 49 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cytolysins Hemolysins Fire coral Millepora complanata Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Cytolysins Hemolysins Fire coral Millepora complanata Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Alejandro García-Arredondo Luis J Murillo-Esquivel Alejandra Rojas Judith Sanchez-Rodriguez Characteristics of hemolytic activity induced by the aqueous extract of the Mexican fire coral Millepora complanata |
topic_facet |
Cytolysins Hemolysins Fire coral Millepora complanata Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
BackgroundMillepora complanata is a plate-like fire coral common throughout the Caribbean. Contact with this species usually provokes burning pain, erythema and urticariform lesions. Our previous study suggested that the aqueous extract of M. complanata contains non-protein hemolysins that are soluble in water and ethanol. In general, the local damage induced by cnidarian venoms has been associated with hemolysins. The characterization of the effects of these components is important for the understanding of the defense mechanisms of fire corals. In addition, this information could lead to better care for victims of envenomation accidents.Methods An ethanolic extract from the lyophilized aqueous extract was prepared and its hemolytic activity was compared with the hemolysis induced by the denatured aqueous extract. Based on the finding that ethanol failed to induce nematocyst discharge, ethanolic extracts were prepared from artificially bleached and normal M. complanata fragments and their hemolytic activity was tested in order to obtain information about the source of the heat-stable hemolysins.Results Rodent erythrocytes were more susceptible to the aqueous extract than chicken and human erythrocytes. Hemolytic activity started at ten minutes of incubation and was relatively stable within the range of 28-50°C. When the aqueous extract was preincubated at temperatures over 60°C, hemolytic activity was significantly reduced. The denatured extract induced a slow hemolytic activity (HU50= 1,050.00 ± 45.85 μg/mL), detectable four hours after incubation, which was similar to that induced by the ethanolic extract prepared from the aqueous extract (HU50= 1,167.00 ± 54.95 μg/mL). No significant differences were observed between hemolysis induced by ethanolic extracts from bleached and normal fragments, although both activities were more potent than hemolysis induced by the denatured extract.Conclusions The results showed that the aqueous extract of M. complanata possesses one or more powerful heat-labile hemolytic ... |
format |
Article in Journal/Newspaper |
author |
Alejandro García-Arredondo Luis J Murillo-Esquivel Alejandra Rojas Judith Sanchez-Rodriguez |
author_facet |
Alejandro García-Arredondo Luis J Murillo-Esquivel Alejandra Rojas Judith Sanchez-Rodriguez |
author_sort |
Alejandro García-Arredondo |
title |
Characteristics of hemolytic activity induced by the aqueous extract of the Mexican fire coral Millepora complanata |
title_short |
Characteristics of hemolytic activity induced by the aqueous extract of the Mexican fire coral Millepora complanata |
title_full |
Characteristics of hemolytic activity induced by the aqueous extract of the Mexican fire coral Millepora complanata |
title_fullStr |
Characteristics of hemolytic activity induced by the aqueous extract of the Mexican fire coral Millepora complanata |
title_full_unstemmed |
Characteristics of hemolytic activity induced by the aqueous extract of the Mexican fire coral Millepora complanata |
title_sort |
characteristics of hemolytic activity induced by the aqueous extract of the mexican fire coral millepora complanata |
publisher |
SciELO |
publishDate |
2014 |
url |
https://doi.org/10.1186/1678-9199-20-49 https://doaj.org/article/6f2f8f81f8f84e9db492035c4f163a3e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 20, Iss 0 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200339&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-20-49 https://doaj.org/article/6f2f8f81f8f84e9db492035c4f163a3e |
op_doi |
https://doi.org/10.1186/1678-9199-20-49 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
20 |
container_issue |
1 |
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49 |
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1766345189722423296 |