NHP BurkPx: A multiplex serodiagnostic bead assay to monitor Burkholderia pseudomallei exposures in non-human primates
Background Melioidosis is a disease caused by the bacterium Burkholderia pseudomallei, infecting humans and non-human primates (NHP) through contaminated soil or water. World-wide there are an estimated 165,000 human melioidosis cases each year, but recordings of NHP cases are sporadic. Clinical det...
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ftdoajarticles:oai:doaj.org/article:6eeeb6cc26b243debef04e286cd0ac95 2023-05-15T15:16:30+02:00 NHP BurkPx: A multiplex serodiagnostic bead assay to monitor Burkholderia pseudomallei exposures in non-human primates Kimberly R. Celona Austin B. Shannon Derek Sonderegger Jinhee Yi Fernando P. Monroy Christopher Allender Heidie Hornstra Mary B. Barnes Elizabeth S. Didier Rudolf P. Bohm Kathrine Phillippi-Falkenstein Daniel Sanford Paul Keim Erik W. Settles 2023-02-01T00:00:00Z https://doaj.org/article/6eeeb6cc26b243debef04e286cd0ac95 EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9907805/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 https://doaj.org/article/6eeeb6cc26b243debef04e286cd0ac95 PLoS Neglected Tropical Diseases, Vol 17, Iss 2 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles 2023-02-12T01:27:53Z Background Melioidosis is a disease caused by the bacterium Burkholderia pseudomallei, infecting humans and non-human primates (NHP) through contaminated soil or water. World-wide there are an estimated 165,000 human melioidosis cases each year, but recordings of NHP cases are sporadic. Clinical detection of melioidosis in humans is primarily by culturing B. pseudomallei, and there are no standardized detection protocols for NHP. NHP are an important animal model for melioidosis research including clinical trials and development of biodefense countermeasures. Methodology/Principle findings We evaluated the diagnostic potential of the multiple antigen serological assay, BurkPx, in NHP using two sera sets: (i) 115 B. pseudomallei-challenged serum samples from 80 NHP collected each week post-exposure (n = 52) and at euthanasia (n = 47), and (ii) 126 B. pseudomallei-naïve/negative serum samples. We observed early IgM antibody responses to carbohydrate antigens followed by IgG antibody recognition to multiple B. pseudomallei protein antigens during the second week of infection. B. pseudomallei negative serum samples had low to intermediate antibody cross reactivity to the antigens in this assay. Infection time was predicted as the determining factor in the variation of antibody responses, with 77.67% of variation explained by the first component of the principal component analysis. A multiple antigen model generated a binary prediction metric ( Conclusion/Significance We employed a previously standardized assay for humans, the BurkPx assay, and assessed its diagnostic potential for detection of B. pseudomallei exposure in NHP. The assay is expected to be useful for surveillance in NHP colonies, in investigations of suspected accidental releases or exposures, and for identifying vaccine correlates of protection. Author summary Melioidosis is a disease caused by the soil-dwelling bacterium Burkholderia pseudomallei, a Tier 1 Select Agent by the US Centers for Disease Control and Prevention (CDC), with the ability to ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kimberly R. Celona Austin B. Shannon Derek Sonderegger Jinhee Yi Fernando P. Monroy Christopher Allender Heidie Hornstra Mary B. Barnes Elizabeth S. Didier Rudolf P. Bohm Kathrine Phillippi-Falkenstein Daniel Sanford Paul Keim Erik W. Settles NHP BurkPx: A multiplex serodiagnostic bead assay to monitor Burkholderia pseudomallei exposures in non-human primates |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Melioidosis is a disease caused by the bacterium Burkholderia pseudomallei, infecting humans and non-human primates (NHP) through contaminated soil or water. World-wide there are an estimated 165,000 human melioidosis cases each year, but recordings of NHP cases are sporadic. Clinical detection of melioidosis in humans is primarily by culturing B. pseudomallei, and there are no standardized detection protocols for NHP. NHP are an important animal model for melioidosis research including clinical trials and development of biodefense countermeasures. Methodology/Principle findings We evaluated the diagnostic potential of the multiple antigen serological assay, BurkPx, in NHP using two sera sets: (i) 115 B. pseudomallei-challenged serum samples from 80 NHP collected each week post-exposure (n = 52) and at euthanasia (n = 47), and (ii) 126 B. pseudomallei-naïve/negative serum samples. We observed early IgM antibody responses to carbohydrate antigens followed by IgG antibody recognition to multiple B. pseudomallei protein antigens during the second week of infection. B. pseudomallei negative serum samples had low to intermediate antibody cross reactivity to the antigens in this assay. Infection time was predicted as the determining factor in the variation of antibody responses, with 77.67% of variation explained by the first component of the principal component analysis. A multiple antigen model generated a binary prediction metric ( Conclusion/Significance We employed a previously standardized assay for humans, the BurkPx assay, and assessed its diagnostic potential for detection of B. pseudomallei exposure in NHP. The assay is expected to be useful for surveillance in NHP colonies, in investigations of suspected accidental releases or exposures, and for identifying vaccine correlates of protection. Author summary Melioidosis is a disease caused by the soil-dwelling bacterium Burkholderia pseudomallei, a Tier 1 Select Agent by the US Centers for Disease Control and Prevention (CDC), with the ability to ... |
format |
Article in Journal/Newspaper |
author |
Kimberly R. Celona Austin B. Shannon Derek Sonderegger Jinhee Yi Fernando P. Monroy Christopher Allender Heidie Hornstra Mary B. Barnes Elizabeth S. Didier Rudolf P. Bohm Kathrine Phillippi-Falkenstein Daniel Sanford Paul Keim Erik W. Settles |
author_facet |
Kimberly R. Celona Austin B. Shannon Derek Sonderegger Jinhee Yi Fernando P. Monroy Christopher Allender Heidie Hornstra Mary B. Barnes Elizabeth S. Didier Rudolf P. Bohm Kathrine Phillippi-Falkenstein Daniel Sanford Paul Keim Erik W. Settles |
author_sort |
Kimberly R. Celona |
title |
NHP BurkPx: A multiplex serodiagnostic bead assay to monitor Burkholderia pseudomallei exposures in non-human primates |
title_short |
NHP BurkPx: A multiplex serodiagnostic bead assay to monitor Burkholderia pseudomallei exposures in non-human primates |
title_full |
NHP BurkPx: A multiplex serodiagnostic bead assay to monitor Burkholderia pseudomallei exposures in non-human primates |
title_fullStr |
NHP BurkPx: A multiplex serodiagnostic bead assay to monitor Burkholderia pseudomallei exposures in non-human primates |
title_full_unstemmed |
NHP BurkPx: A multiplex serodiagnostic bead assay to monitor Burkholderia pseudomallei exposures in non-human primates |
title_sort |
nhp burkpx: a multiplex serodiagnostic bead assay to monitor burkholderia pseudomallei exposures in non-human primates |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doaj.org/article/6eeeb6cc26b243debef04e286cd0ac95 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 2 (2023) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9907805/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 https://doaj.org/article/6eeeb6cc26b243debef04e286cd0ac95 |
_version_ |
1766346802503614464 |