Specification of blood meals ingested by female sand flies caught in Palestinian foci and identification of their concomitant leishmanial infections.
Since leishmaniases are zoonotic vector-borne diseases transmitted through the bites of infected female sand flies, identification of the sources of imbibed blood meals and the detection and identification of leishmanial DNA in them are important in discerning animal reservoirs, clarifying the epide...
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ftdoajarticles:oai:doaj.org/article:6edf3794a10949d0a1c38a9b72f67caf 2023-05-15T15:17:10+02:00 Specification of blood meals ingested by female sand flies caught in Palestinian foci and identification of their concomitant leishmanial infections. Kifaya Azmi Gabriele Schonian Ziad Abdeen 2020-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008748 https://doaj.org/article/6edf3794a10949d0a1c38a9b72f67caf EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008748 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008748 https://doaj.org/article/6edf3794a10949d0a1c38a9b72f67caf PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008748 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008748 2022-12-31T05:48:39Z Since leishmaniases are zoonotic vector-borne diseases transmitted through the bites of infected female sand flies, identification of the sources of imbibed blood meals and the detection and identification of leishmanial DNA in them are important in discerning animal reservoirs, clarifying the epidemiology and facilitating control of local leishmaniases. CDC light traps, aspirators and sticky paper traps were used to collect sand flies in four Palestinian foci of both, CL and VL. Phlebotomine species identification was based on morphological keys. Female specimens were screened to detect and identify leishmanial infections, using kDNA-PCR and ITS1-PCR, and engorged female specimens were analyzed to identify the origin of their blood meals, using an RDB blood meal assay based on the amplification of the cytochrome b gene (cytb) of vertebrate mitochondrial DNA (mtDNA). Twenty sand fly species, 11 of the genus Phlebotomus and nine the genus Sergentomyia, were identified. The most abundant species was Ph. papatasi (33.7%), followed by Ph. sergenti (21%). Among the 691 female sand fly specimens, 18.5% (128/691) were positive for leishmanial DNA, using the kDNA-PCR and 6.4% (44/691) were positive using the ITS1-PCR. DNA from parasites of the genus Leishmania was identified in only 1.5% of the infected sand flies. That of Leishmania tropica parasites was detected in six female specimens of Ph. sergenti and that of L. major parasites in two female specimens of Ph. papatasi. Interestingly, two engorged females of the species Se. (Neophlebotomus) sp. were positive for L. tropica DNA. Ninety engorged female sand flies of Ph. papatasi and 104 of Ph. sergenti had fed on a large variety of vertebrate hosts such as humans, hyraxes, rats, cows, goats and birds. Regarding blood-meals showing a mixture from different species of animal host, hyrax and rat blood was revealed in 8/104 (7.7%) females of Ph. sergenti. Detection of hyrax blood in engorged female sand flies of the species Ph. sergenti supports the role of hyraxes being ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 10 e0008748 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kifaya Azmi Gabriele Schonian Ziad Abdeen Specification of blood meals ingested by female sand flies caught in Palestinian foci and identification of their concomitant leishmanial infections. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Since leishmaniases are zoonotic vector-borne diseases transmitted through the bites of infected female sand flies, identification of the sources of imbibed blood meals and the detection and identification of leishmanial DNA in them are important in discerning animal reservoirs, clarifying the epidemiology and facilitating control of local leishmaniases. CDC light traps, aspirators and sticky paper traps were used to collect sand flies in four Palestinian foci of both, CL and VL. Phlebotomine species identification was based on morphological keys. Female specimens were screened to detect and identify leishmanial infections, using kDNA-PCR and ITS1-PCR, and engorged female specimens were analyzed to identify the origin of their blood meals, using an RDB blood meal assay based on the amplification of the cytochrome b gene (cytb) of vertebrate mitochondrial DNA (mtDNA). Twenty sand fly species, 11 of the genus Phlebotomus and nine the genus Sergentomyia, were identified. The most abundant species was Ph. papatasi (33.7%), followed by Ph. sergenti (21%). Among the 691 female sand fly specimens, 18.5% (128/691) were positive for leishmanial DNA, using the kDNA-PCR and 6.4% (44/691) were positive using the ITS1-PCR. DNA from parasites of the genus Leishmania was identified in only 1.5% of the infected sand flies. That of Leishmania tropica parasites was detected in six female specimens of Ph. sergenti and that of L. major parasites in two female specimens of Ph. papatasi. Interestingly, two engorged females of the species Se. (Neophlebotomus) sp. were positive for L. tropica DNA. Ninety engorged female sand flies of Ph. papatasi and 104 of Ph. sergenti had fed on a large variety of vertebrate hosts such as humans, hyraxes, rats, cows, goats and birds. Regarding blood-meals showing a mixture from different species of animal host, hyrax and rat blood was revealed in 8/104 (7.7%) females of Ph. sergenti. Detection of hyrax blood in engorged female sand flies of the species Ph. sergenti supports the role of hyraxes being ... |
format |
Article in Journal/Newspaper |
author |
Kifaya Azmi Gabriele Schonian Ziad Abdeen |
author_facet |
Kifaya Azmi Gabriele Schonian Ziad Abdeen |
author_sort |
Kifaya Azmi |
title |
Specification of blood meals ingested by female sand flies caught in Palestinian foci and identification of their concomitant leishmanial infections. |
title_short |
Specification of blood meals ingested by female sand flies caught in Palestinian foci and identification of their concomitant leishmanial infections. |
title_full |
Specification of blood meals ingested by female sand flies caught in Palestinian foci and identification of their concomitant leishmanial infections. |
title_fullStr |
Specification of blood meals ingested by female sand flies caught in Palestinian foci and identification of their concomitant leishmanial infections. |
title_full_unstemmed |
Specification of blood meals ingested by female sand flies caught in Palestinian foci and identification of their concomitant leishmanial infections. |
title_sort |
specification of blood meals ingested by female sand flies caught in palestinian foci and identification of their concomitant leishmanial infections. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008748 https://doaj.org/article/6edf3794a10949d0a1c38a9b72f67caf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008748 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008748 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008748 https://doaj.org/article/6edf3794a10949d0a1c38a9b72f67caf |
op_doi |
https://doi.org/10.1371/journal.pntd.0008748 |
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PLOS Neglected Tropical Diseases |
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14 |
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10 |
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e0008748 |
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1766347433168601088 |