High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada
Abstract Background Species of Trichinella are globally important foodborne parasites infecting a number of domestic and wild vertebrates, including humans. Free-ranging carnivores can act as sentinel species for detection of Trichinella spp. Knowledge of the epidemiology of these parasites may help...
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ftdoajarticles:oai:doaj.org/article:6ecb734bf6604f9c923af4f1f4e73e82 2023-05-15T16:32:18+02:00 High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada Rajnish Sharma N. Jane Harms Piia M. Kukka Thomas S. Jung Sarah E. Parker Sasha Ross Peter Thompson Benjamin Rosenthal Eric P. Hoberg Emily J. Jenkins 2021-03-01T00:00:00Z https://doi.org/10.1186/s13071-021-04636-2 https://doaj.org/article/6ecb734bf6604f9c923af4f1f4e73e82 EN eng BMC https://doi.org/10.1186/s13071-021-04636-2 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-021-04636-2 1756-3305 https://doaj.org/article/6ecb734bf6604f9c923af4f1f4e73e82 Parasites & Vectors, Vol 14, Iss 1, Pp 1-9 (2021) Trichinella spp Wolverine Canada Prevalence T. nativa Trichinella T6 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s13071-021-04636-2 2022-12-31T06:17:23Z Abstract Background Species of Trichinella are globally important foodborne parasites infecting a number of domestic and wild vertebrates, including humans. Free-ranging carnivores can act as sentinel species for detection of Trichinella spp. Knowledge of the epidemiology of these parasites may help prevent Trichinella spp. infections in northern Canadian animals and people. Previous research on Trichinella spp. in wildlife from Yukon did not identify risk factors associated with infection, or the diversity and identity of species of Trichinella in regional circulation, based on geographically extensive sampling with large sample sizes. Methods In a cross-sectional study, we determined the prevalence, infection intensity, risk factors, and species or genotypes of Trichinella in wolverine (Gulo gulo) in two regions of Yukon, Canada, from 2013–2017. A double separatory funnel digestion method followed by mutiplex PCR and PCR-RFLP were used to recover and identify species of Trichinella, respectively. Results We found larvae of Trichinella in the tongues of 78% (95% CI 73–82) of 338 wolverine sampled. The odds of adult (≥ 2 years) and yearling (1 year) wolverine being Trichinella spp.-positive were four and two times higher, respectively, compared to juveniles (<1 year). The odds of Trichinella spp. presence were three times higher in wolverine from southeast than northwest Yukon. The mean intensity of infection was 22.6 ± 39 (SD, range 0.1–295) larvae per gram. Trichinella T6 was the predominant genotype (76%), followed by T. nativa (8%); mixed infections with Trichinella T6 and T. nativa (12%) were observed. In addition, T. spiralis was detected in one wolverine. Out of 22 isolates initially identified as T. nativa in multiplex PCR, 14 were analyzed by PCR-RFLP to distinguish them from T. chanchalensis, a recently discovered cryptic species, which cannot be distinguished from the T. nativa on multiplex PCR. Ten isolates were identified either as T. chanchalensis alone (n = 7), or mixed infection with T. ... Article in Journal/Newspaper Gulo gulo Yukon Directory of Open Access Journals: DOAJ Articles Yukon Canada Parasites & Vectors 14 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Trichinella spp Wolverine Canada Prevalence T. nativa Trichinella T6 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Trichinella spp Wolverine Canada Prevalence T. nativa Trichinella T6 Infectious and parasitic diseases RC109-216 Rajnish Sharma N. Jane Harms Piia M. Kukka Thomas S. Jung Sarah E. Parker Sasha Ross Peter Thompson Benjamin Rosenthal Eric P. Hoberg Emily J. Jenkins High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada |
topic_facet |
Trichinella spp Wolverine Canada Prevalence T. nativa Trichinella T6 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Species of Trichinella are globally important foodborne parasites infecting a number of domestic and wild vertebrates, including humans. Free-ranging carnivores can act as sentinel species for detection of Trichinella spp. Knowledge of the epidemiology of these parasites may help prevent Trichinella spp. infections in northern Canadian animals and people. Previous research on Trichinella spp. in wildlife from Yukon did not identify risk factors associated with infection, or the diversity and identity of species of Trichinella in regional circulation, based on geographically extensive sampling with large sample sizes. Methods In a cross-sectional study, we determined the prevalence, infection intensity, risk factors, and species or genotypes of Trichinella in wolverine (Gulo gulo) in two regions of Yukon, Canada, from 2013–2017. A double separatory funnel digestion method followed by mutiplex PCR and PCR-RFLP were used to recover and identify species of Trichinella, respectively. Results We found larvae of Trichinella in the tongues of 78% (95% CI 73–82) of 338 wolverine sampled. The odds of adult (≥ 2 years) and yearling (1 year) wolverine being Trichinella spp.-positive were four and two times higher, respectively, compared to juveniles (<1 year). The odds of Trichinella spp. presence were three times higher in wolverine from southeast than northwest Yukon. The mean intensity of infection was 22.6 ± 39 (SD, range 0.1–295) larvae per gram. Trichinella T6 was the predominant genotype (76%), followed by T. nativa (8%); mixed infections with Trichinella T6 and T. nativa (12%) were observed. In addition, T. spiralis was detected in one wolverine. Out of 22 isolates initially identified as T. nativa in multiplex PCR, 14 were analyzed by PCR-RFLP to distinguish them from T. chanchalensis, a recently discovered cryptic species, which cannot be distinguished from the T. nativa on multiplex PCR. Ten isolates were identified either as T. chanchalensis alone (n = 7), or mixed infection with T. ... |
format |
Article in Journal/Newspaper |
author |
Rajnish Sharma N. Jane Harms Piia M. Kukka Thomas S. Jung Sarah E. Parker Sasha Ross Peter Thompson Benjamin Rosenthal Eric P. Hoberg Emily J. Jenkins |
author_facet |
Rajnish Sharma N. Jane Harms Piia M. Kukka Thomas S. Jung Sarah E. Parker Sasha Ross Peter Thompson Benjamin Rosenthal Eric P. Hoberg Emily J. Jenkins |
author_sort |
Rajnish Sharma |
title |
High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada |
title_short |
High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada |
title_full |
High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada |
title_fullStr |
High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada |
title_full_unstemmed |
High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada |
title_sort |
high prevalence, intensity, and genetic diversity of trichinella spp. in wolverine (gulo gulo) from yukon, canada |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s13071-021-04636-2 https://doaj.org/article/6ecb734bf6604f9c923af4f1f4e73e82 |
geographic |
Yukon Canada |
geographic_facet |
Yukon Canada |
genre |
Gulo gulo Yukon |
genre_facet |
Gulo gulo Yukon |
op_source |
Parasites & Vectors, Vol 14, Iss 1, Pp 1-9 (2021) |
op_relation |
https://doi.org/10.1186/s13071-021-04636-2 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-021-04636-2 1756-3305 https://doaj.org/article/6ecb734bf6604f9c923af4f1f4e73e82 |
op_doi |
https://doi.org/10.1186/s13071-021-04636-2 |
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Parasites & Vectors |
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14 |
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