A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities
Antarctic microbes are important agents for evolutionary adaptation and natural resource of bioactive compounds, harboring the particular metabolic pathways to biosynthesize natural products. However, not much is known on symbiotic microbiomes of fish in the Antarctic zone. In the present study, the...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2023
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fmicb.2022.1085063 https://doaj.org/article/6ebada3a355b42b2a67d8434251ae96d |
id |
ftdoajarticles:oai:doaj.org/article:6ebada3a355b42b2a67d8434251ae96d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:6ebada3a355b42b2a67d8434251ae96d 2023-05-15T13:33:50+02:00 A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities Yu Xiao Fangfang Yan Yukun Cui Jiangtao Du Guangzhao Hu Wanying Zhai Rulong Liu Zhizhen Zhang Jiasong Fang Liangbiao Chen Xi Yu 2023-01-01T00:00:00Z https://doi.org/10.3389/fmicb.2022.1085063 https://doaj.org/article/6ebada3a355b42b2a67d8434251ae96d EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmicb.2022.1085063/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2022.1085063 https://doaj.org/article/6ebada3a355b42b2a67d8434251ae96d Frontiers in Microbiology, Vol 13 (2023) Antarctic fish symbiotic bacteria Serratia genome bioactive metabolites Microbiology QR1-502 article 2023 ftdoajarticles https://doi.org/10.3389/fmicb.2022.1085063 2023-01-15T01:24:49Z Antarctic microbes are important agents for evolutionary adaptation and natural resource of bioactive compounds, harboring the particular metabolic pathways to biosynthesize natural products. However, not much is known on symbiotic microbiomes of fish in the Antarctic zone. In the present study, the culture method and whole-genome sequencing were performed. Natural product analyses were carried out to determine the biosynthetic potential. We report the isolation and identification of a symbiotic bacterium Serratia myotis L7-1, that is highly adaptive and resides within Antarctic fish, Trematomus bernacchii. As revealed by genomic analyses, Antarctic strain S. myotis L7-1 possesses carbohydrate-active enzymes (CAZymes), biosynthetic gene clusters (BGCs), stress response genes, antibiotic resistant genes (ARGs), and a complete type IV secretion system which could facilitate competition and colonization in the extreme Antarctic environment. The identification of microbiome gene clusters indicates the biosynthetic potential of bioactive compounds. Based on bioactivity-guided fractionation, serranticin was purified and identified as the bioactive compound, showing significant antibacterial and antitumor activity. The serranticin gene cluster was identified and located on the chrome. Furthermore, the multidrug resistance and strong bacterial antagonism contribute competitive advantages in ecological niches. Our results highlight the existence of a symbiotic bacterium in Antarctic fish largely represented by bioactive natural products and the adaptability to survive in the fish living in Antarctic oceans. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Frontiers in Microbiology 13 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctic fish symbiotic bacteria Serratia genome bioactive metabolites Microbiology QR1-502 |
spellingShingle |
Antarctic fish symbiotic bacteria Serratia genome bioactive metabolites Microbiology QR1-502 Yu Xiao Fangfang Yan Yukun Cui Jiangtao Du Guangzhao Hu Wanying Zhai Rulong Liu Zhizhen Zhang Jiasong Fang Liangbiao Chen Xi Yu A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
topic_facet |
Antarctic fish symbiotic bacteria Serratia genome bioactive metabolites Microbiology QR1-502 |
description |
Antarctic microbes are important agents for evolutionary adaptation and natural resource of bioactive compounds, harboring the particular metabolic pathways to biosynthesize natural products. However, not much is known on symbiotic microbiomes of fish in the Antarctic zone. In the present study, the culture method and whole-genome sequencing were performed. Natural product analyses were carried out to determine the biosynthetic potential. We report the isolation and identification of a symbiotic bacterium Serratia myotis L7-1, that is highly adaptive and resides within Antarctic fish, Trematomus bernacchii. As revealed by genomic analyses, Antarctic strain S. myotis L7-1 possesses carbohydrate-active enzymes (CAZymes), biosynthetic gene clusters (BGCs), stress response genes, antibiotic resistant genes (ARGs), and a complete type IV secretion system which could facilitate competition and colonization in the extreme Antarctic environment. The identification of microbiome gene clusters indicates the biosynthetic potential of bioactive compounds. Based on bioactivity-guided fractionation, serranticin was purified and identified as the bioactive compound, showing significant antibacterial and antitumor activity. The serranticin gene cluster was identified and located on the chrome. Furthermore, the multidrug resistance and strong bacterial antagonism contribute competitive advantages in ecological niches. Our results highlight the existence of a symbiotic bacterium in Antarctic fish largely represented by bioactive natural products and the adaptability to survive in the fish living in Antarctic oceans. |
format |
Article in Journal/Newspaper |
author |
Yu Xiao Fangfang Yan Yukun Cui Jiangtao Du Guangzhao Hu Wanying Zhai Rulong Liu Zhizhen Zhang Jiasong Fang Liangbiao Chen Xi Yu |
author_facet |
Yu Xiao Fangfang Yan Yukun Cui Jiangtao Du Guangzhao Hu Wanying Zhai Rulong Liu Zhizhen Zhang Jiasong Fang Liangbiao Chen Xi Yu |
author_sort |
Yu Xiao |
title |
A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
title_short |
A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
title_full |
A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
title_fullStr |
A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
title_full_unstemmed |
A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
title_sort |
symbiotic bacterium of antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities |
publisher |
Frontiers Media S.A. |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmicb.2022.1085063 https://doaj.org/article/6ebada3a355b42b2a67d8434251ae96d |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Frontiers in Microbiology, Vol 13 (2023) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmicb.2022.1085063/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2022.1085063 https://doaj.org/article/6ebada3a355b42b2a67d8434251ae96d |
op_doi |
https://doi.org/10.3389/fmicb.2022.1085063 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
_version_ |
1766046442276782080 |