Effect οf elicitοrs οn cell suspensiοn culture οf Urginea maritima L. tοwards prοductiοn οf prοscillaridin A
Background: Urginea maritima (L.f.) Baker (Hyacinthaceae) is a perennial bulbous medicinal plant that is currently at risk of extinction. Squill (white sea onion) is an analogous cardiotonic to digitalis. The purpose of the current work was to assess the optimal growth conditions for Urginea cells t...
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ftdoajarticles:oai:doaj.org/article:6ea7f01217f640e9bfb206fc3b3595df 2023-05-15T18:43:53+02:00 Effect οf elicitοrs οn cell suspensiοn culture οf Urginea maritima L. tοwards prοductiοn οf prοscillaridin A Heba Shahin Atef A. El-Hela Mahmοud I. Nasr Ghada M. Nasr 2022-01-01T00:00:00Z https://doi.org/10.31083/j.fbl2702045 https://doaj.org/article/6ea7f01217f640e9bfb206fc3b3595df EN eng IMR Press https://www.imrpress.com/journal/FBL/27/2/10.31083/j.fbl2702045 https://doaj.org/toc/2768-6701 2768-6701 doi:10.31083/j.fbl2702045 https://doaj.org/article/6ea7f01217f640e9bfb206fc3b3595df Frontiers in Bioscience-Landmark, Vol 27, Iss 2, p 045 (2022) u. maritima proscillaridin a elicitors suspension culture methyle jasmonate Biochemistry QD415-436 Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.31083/j.fbl2702045 2022-12-31T12:01:14Z Background: Urginea maritima (L.f.) Baker (Hyacinthaceae) is a perennial bulbous medicinal plant that is currently at risk of extinction. Squill (white sea onion) is an analogous cardiotonic to digitalis. The purpose of the current work was to assess the optimal growth conditions for Urginea cells to synthesize the cardiac glycoside proscillaridin A by involving illumination, carbon source, methyl jasmonate (MJ), and culture system. Results: When cells were cultured for 28 days at 21 ± 2 °C in the dark on Murashige and Skoog (MS) media containing 1 mg/L 2, 4-D and 0.5 mg/L Kin and 30 g/L sucrose, the cell proliferation, and proscillaridin A synthesis were effectively controlled. At low concentrations, MJ stimulated the synthesis of proscillaridin A (PsA). High-performance liquid chromatography (HPLC) analysis of suspension extracts demonstrated that the callus maintained in MS media enriched with 1 mg/L 2, 4-D and 0.5 mg/L Kin yielded a greater formation of Proscillaridin A (141.31 mg/g DW) than untreated plants. Conclusions: The results indicate that in vitro cultures of U. maritima may be an excellent source of proscillaridin A. Moreover, it is one of the most important cardiac glycoside, which has been found to exhibit anticancer activities. Suspension cultures of Urginea cells could be as highly productive as a callus culture. Article in Journal/Newspaper White Sea Directory of Open Access Journals: DOAJ Articles White Sea Frontiers in Bioscience-Landmark 27 2 045 |
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English |
topic |
u. maritima proscillaridin a elicitors suspension culture methyle jasmonate Biochemistry QD415-436 Biology (General) QH301-705.5 |
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u. maritima proscillaridin a elicitors suspension culture methyle jasmonate Biochemistry QD415-436 Biology (General) QH301-705.5 Heba Shahin Atef A. El-Hela Mahmοud I. Nasr Ghada M. Nasr Effect οf elicitοrs οn cell suspensiοn culture οf Urginea maritima L. tοwards prοductiοn οf prοscillaridin A |
topic_facet |
u. maritima proscillaridin a elicitors suspension culture methyle jasmonate Biochemistry QD415-436 Biology (General) QH301-705.5 |
description |
Background: Urginea maritima (L.f.) Baker (Hyacinthaceae) is a perennial bulbous medicinal plant that is currently at risk of extinction. Squill (white sea onion) is an analogous cardiotonic to digitalis. The purpose of the current work was to assess the optimal growth conditions for Urginea cells to synthesize the cardiac glycoside proscillaridin A by involving illumination, carbon source, methyl jasmonate (MJ), and culture system. Results: When cells were cultured for 28 days at 21 ± 2 °C in the dark on Murashige and Skoog (MS) media containing 1 mg/L 2, 4-D and 0.5 mg/L Kin and 30 g/L sucrose, the cell proliferation, and proscillaridin A synthesis were effectively controlled. At low concentrations, MJ stimulated the synthesis of proscillaridin A (PsA). High-performance liquid chromatography (HPLC) analysis of suspension extracts demonstrated that the callus maintained in MS media enriched with 1 mg/L 2, 4-D and 0.5 mg/L Kin yielded a greater formation of Proscillaridin A (141.31 mg/g DW) than untreated plants. Conclusions: The results indicate that in vitro cultures of U. maritima may be an excellent source of proscillaridin A. Moreover, it is one of the most important cardiac glycoside, which has been found to exhibit anticancer activities. Suspension cultures of Urginea cells could be as highly productive as a callus culture. |
format |
Article in Journal/Newspaper |
author |
Heba Shahin Atef A. El-Hela Mahmοud I. Nasr Ghada M. Nasr |
author_facet |
Heba Shahin Atef A. El-Hela Mahmοud I. Nasr Ghada M. Nasr |
author_sort |
Heba Shahin |
title |
Effect οf elicitοrs οn cell suspensiοn culture οf Urginea maritima L. tοwards prοductiοn οf prοscillaridin A |
title_short |
Effect οf elicitοrs οn cell suspensiοn culture οf Urginea maritima L. tοwards prοductiοn οf prοscillaridin A |
title_full |
Effect οf elicitοrs οn cell suspensiοn culture οf Urginea maritima L. tοwards prοductiοn οf prοscillaridin A |
title_fullStr |
Effect οf elicitοrs οn cell suspensiοn culture οf Urginea maritima L. tοwards prοductiοn οf prοscillaridin A |
title_full_unstemmed |
Effect οf elicitοrs οn cell suspensiοn culture οf Urginea maritima L. tοwards prοductiοn οf prοscillaridin A |
title_sort |
effect οf elicitοrs οn cell suspensiοn culture οf urginea maritima l. tοwards prοductiοn οf prοscillaridin a |
publisher |
IMR Press |
publishDate |
2022 |
url |
https://doi.org/10.31083/j.fbl2702045 https://doaj.org/article/6ea7f01217f640e9bfb206fc3b3595df |
geographic |
White Sea |
geographic_facet |
White Sea |
genre |
White Sea |
genre_facet |
White Sea |
op_source |
Frontiers in Bioscience-Landmark, Vol 27, Iss 2, p 045 (2022) |
op_relation |
https://www.imrpress.com/journal/FBL/27/2/10.31083/j.fbl2702045 https://doaj.org/toc/2768-6701 2768-6701 doi:10.31083/j.fbl2702045 https://doaj.org/article/6ea7f01217f640e9bfb206fc3b3595df |
op_doi |
https://doi.org/10.31083/j.fbl2702045 |
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Frontiers in Bioscience-Landmark |
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27 |
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2 |
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