Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1
Abstract Background Leishmania RNA virus-1 (LRV1) is a double-stranded RNA virus identified in 20–25% of Viannia—species endemic to Latin America, and is believed to accelerate cutaneous to mucosal leishmaniasis over time. Our objective was to quantify known virulence factor (VF) RNA transcript expr...
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ftdoajarticles:oai:doaj.org/article:6e91d6af5f2e43d39f134b14321ac74f 2023-05-15T15:17:22+02:00 Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1 Ruwandi Kariyawasam Avinash N. Mukkala Rachel Lau Braulio M. Valencia Alejandro Llanos-Cuentas Andrea K. Boggild 2019-04-01T00:00:00Z https://doi.org/10.1186/s41182-019-0153-x https://doaj.org/article/6e91d6af5f2e43d39f134b14321ac74f EN eng BMC http://link.springer.com/article/10.1186/s41182-019-0153-x https://doaj.org/toc/1349-4147 doi:10.1186/s41182-019-0153-x 1349-4147 https://doaj.org/article/6e91d6af5f2e43d39f134b14321ac74f Tropical Medicine and Health, Vol 47, Iss 1, Pp 1-9 (2019) American tegumentary leishmaniasis Leishmania Viannia braziliensis Leishmania RNA Virus-1 (LRV1) Virulence factor Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.1186/s41182-019-0153-x 2022-12-31T13:01:39Z Abstract Background Leishmania RNA virus-1 (LRV1) is a double-stranded RNA virus identified in 20–25% of Viannia—species endemic to Latin America, and is believed to accelerate cutaneous to mucosal leishmaniasis over time. Our objective was to quantify known virulence factor (VF) RNA transcript expression according to LRV1 status, causative species, and isolate source. Methods Eight cultured isolates of Leishmania were used, four of which were LRV1-positive (Leishmania Viannia braziliensis [n = 1], L. (V.) guyanensis [n = 1], L. (V.) panamensis [n = 2]), and four were LRV1-negative (L. (V.) panamensis [n = 3], L. (V.) braziliensis [n = 1]). Promastigotes were inoculated into macrophage cultures, and harvested at 24 and 48 h. RNA transcript expression of hsp23, hsp70, hsp90, hsp100, mpi, cpb, and gp63 were quantified by qPCR. Results RNA transcript expression of hsp100 (p = 0.012), cpb (p = 0.016), and mpi (p = 0.022) showed significant increases from baseline pure culture expression to 24- and 48-h post-macrophage infection, whereas hsp70 (p = 0.004) was significantly decreased. A trend toward increased transcript expression of hsp100 at baseline in isolates of L. (V.) panamensis was noted. Pooled VF RNA transcript expression by L. (V.) panamensis isolates was lower than that of L. (V.) braziliensis and L. (V.) guyananesis at 24 h (p = 0.03). VF RNA transcript expression did not differ by LRV1 status, or source of cultured isolate at baseline, 24, or 48 h; however, a trend toward increased VF RNA transcript expression of 2.71- and 1.93-fold change of mpi (p = 0.11) and hsp90 (p = 0.11), respectively, in LRV1 negative isolates was noted. Similarly, a trend toward lower levels of overall VF RNA transcript expression in clinical isolates (1.15-fold change) compared to ATCC® strains at 24 h was noted (p = 0.07). Conclusions Our findings suggest that known VF RNA transcript expression may be affected by the process of macrophage infection. We were unable to demonstrate definitively that LRV-1 presence affected VF RNA ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Tropical Medicine and Health 47 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
American tegumentary leishmaniasis Leishmania Viannia braziliensis Leishmania RNA Virus-1 (LRV1) Virulence factor Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
American tegumentary leishmaniasis Leishmania Viannia braziliensis Leishmania RNA Virus-1 (LRV1) Virulence factor Arctic medicine. Tropical medicine RC955-962 Ruwandi Kariyawasam Avinash N. Mukkala Rachel Lau Braulio M. Valencia Alejandro Llanos-Cuentas Andrea K. Boggild Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1 |
topic_facet |
American tegumentary leishmaniasis Leishmania Viannia braziliensis Leishmania RNA Virus-1 (LRV1) Virulence factor Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract Background Leishmania RNA virus-1 (LRV1) is a double-stranded RNA virus identified in 20–25% of Viannia—species endemic to Latin America, and is believed to accelerate cutaneous to mucosal leishmaniasis over time. Our objective was to quantify known virulence factor (VF) RNA transcript expression according to LRV1 status, causative species, and isolate source. Methods Eight cultured isolates of Leishmania were used, four of which were LRV1-positive (Leishmania Viannia braziliensis [n = 1], L. (V.) guyanensis [n = 1], L. (V.) panamensis [n = 2]), and four were LRV1-negative (L. (V.) panamensis [n = 3], L. (V.) braziliensis [n = 1]). Promastigotes were inoculated into macrophage cultures, and harvested at 24 and 48 h. RNA transcript expression of hsp23, hsp70, hsp90, hsp100, mpi, cpb, and gp63 were quantified by qPCR. Results RNA transcript expression of hsp100 (p = 0.012), cpb (p = 0.016), and mpi (p = 0.022) showed significant increases from baseline pure culture expression to 24- and 48-h post-macrophage infection, whereas hsp70 (p = 0.004) was significantly decreased. A trend toward increased transcript expression of hsp100 at baseline in isolates of L. (V.) panamensis was noted. Pooled VF RNA transcript expression by L. (V.) panamensis isolates was lower than that of L. (V.) braziliensis and L. (V.) guyananesis at 24 h (p = 0.03). VF RNA transcript expression did not differ by LRV1 status, or source of cultured isolate at baseline, 24, or 48 h; however, a trend toward increased VF RNA transcript expression of 2.71- and 1.93-fold change of mpi (p = 0.11) and hsp90 (p = 0.11), respectively, in LRV1 negative isolates was noted. Similarly, a trend toward lower levels of overall VF RNA transcript expression in clinical isolates (1.15-fold change) compared to ATCC® strains at 24 h was noted (p = 0.07). Conclusions Our findings suggest that known VF RNA transcript expression may be affected by the process of macrophage infection. We were unable to demonstrate definitively that LRV-1 presence affected VF RNA ... |
format |
Article in Journal/Newspaper |
author |
Ruwandi Kariyawasam Avinash N. Mukkala Rachel Lau Braulio M. Valencia Alejandro Llanos-Cuentas Andrea K. Boggild |
author_facet |
Ruwandi Kariyawasam Avinash N. Mukkala Rachel Lau Braulio M. Valencia Alejandro Llanos-Cuentas Andrea K. Boggild |
author_sort |
Ruwandi Kariyawasam |
title |
Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1 |
title_short |
Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1 |
title_full |
Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1 |
title_fullStr |
Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1 |
title_full_unstemmed |
Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1 |
title_sort |
virulence factor rna transcript expression in the leishmania viannia subgenus: influence of species, isolate source, and leishmania rna virus-1 |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s41182-019-0153-x https://doaj.org/article/6e91d6af5f2e43d39f134b14321ac74f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Tropical Medicine and Health, Vol 47, Iss 1, Pp 1-9 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s41182-019-0153-x https://doaj.org/toc/1349-4147 doi:10.1186/s41182-019-0153-x 1349-4147 https://doaj.org/article/6e91d6af5f2e43d39f134b14321ac74f |
op_doi |
https://doi.org/10.1186/s41182-019-0153-x |
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Tropical Medicine and Health |
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47 |
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1 |
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1766347607039279104 |