Generalist Eimeria species in rodents: Multilocus analyses indicate inadequate resolution of established markers
Abstract Intracellular parasites of the genus Eimeria are described as tissue/host‐specific. Phylogenetic classification of rodent Eimeria suggested that some species have a broader host range than previously assumed. We explore whether Eimeria spp. infecting house mice are misclassified by the most...
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ftdoajarticles:oai:doaj.org/article:6e83fa8454b14bbaad22291f27e1451a 2023-05-15T17:12:37+02:00 Generalist Eimeria species in rodents: Multilocus analyses indicate inadequate resolution of established markers Víctor Hugo Jarquín‐Díaz Alice Balard Anna Mácová Jenny Jost Tabea Roth von Szepesbéla Karin Berktold Steffen Tank Jana Kvičerová Emanuel Heitlinger 2020-02-01T00:00:00Z https://doi.org/10.1002/ece3.5992 https://doaj.org/article/6e83fa8454b14bbaad22291f27e1451a EN eng Wiley https://doi.org/10.1002/ece3.5992 https://doaj.org/toc/2045-7758 2045-7758 doi:10.1002/ece3.5992 https://doaj.org/article/6e83fa8454b14bbaad22291f27e1451a Ecology and Evolution, Vol 10, Iss 3, Pp 1378-1389 (2020) 18S COI Eimeria multilocus sequence typing phylogenetics rodents Ecology QH540-549.5 article 2020 ftdoajarticles https://doi.org/10.1002/ece3.5992 2022-12-31T16:36:38Z Abstract Intracellular parasites of the genus Eimeria are described as tissue/host‐specific. Phylogenetic classification of rodent Eimeria suggested that some species have a broader host range than previously assumed. We explore whether Eimeria spp. infecting house mice are misclassified by the most widely used molecular markers due to a lack of resolution, or whether, instead, these parasite species are indeed infecting multiple host species. With the commonly used markers (18S/COI), we recovered monophyletic clades of E. falciformis and E. vermiformis from Mus that included E. apionodes identified in other rodent host species (Apodemus spp., Myodes glareolus, and Microtus arvalis). A lack of internal resolution in these clades could suggest the existence of a species complex with a wide host range infecting murid and cricetid rodents. We question, however, the power of COI and 18S markers to provide adequate resolution for assessing host specificity. In addition to the rarely used marker ORF470 from the apicoplast genome, we present multilocus genotyping as an alternative approach. Phylogenetic analysis of 35 nuclear markers differentiated E. falciformis from house mice from isolates from Apodemus hosts. Isolates of E. vermiformis from Mus are still found in clusters interspersed with non‐Mus isolates, even with this high‐resolution data. In conclusion, we show that species‐level resolution should not be assumed for COI and 18S markers in coccidia. Host–parasite cospeciation at shallow phylogenetic nodes, as well as contemporary coccidian host ranges more generally, is still open questions that need to be addressed using novel genetic markers with higher resolution. Article in Journal/Newspaper Microtus arvalis Directory of Open Access Journals: DOAJ Articles Ecology and Evolution 10 3 1378 1389 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
18S COI Eimeria multilocus sequence typing phylogenetics rodents Ecology QH540-549.5 |
spellingShingle |
18S COI Eimeria multilocus sequence typing phylogenetics rodents Ecology QH540-549.5 Víctor Hugo Jarquín‐Díaz Alice Balard Anna Mácová Jenny Jost Tabea Roth von Szepesbéla Karin Berktold Steffen Tank Jana Kvičerová Emanuel Heitlinger Generalist Eimeria species in rodents: Multilocus analyses indicate inadequate resolution of established markers |
topic_facet |
18S COI Eimeria multilocus sequence typing phylogenetics rodents Ecology QH540-549.5 |
description |
Abstract Intracellular parasites of the genus Eimeria are described as tissue/host‐specific. Phylogenetic classification of rodent Eimeria suggested that some species have a broader host range than previously assumed. We explore whether Eimeria spp. infecting house mice are misclassified by the most widely used molecular markers due to a lack of resolution, or whether, instead, these parasite species are indeed infecting multiple host species. With the commonly used markers (18S/COI), we recovered monophyletic clades of E. falciformis and E. vermiformis from Mus that included E. apionodes identified in other rodent host species (Apodemus spp., Myodes glareolus, and Microtus arvalis). A lack of internal resolution in these clades could suggest the existence of a species complex with a wide host range infecting murid and cricetid rodents. We question, however, the power of COI and 18S markers to provide adequate resolution for assessing host specificity. In addition to the rarely used marker ORF470 from the apicoplast genome, we present multilocus genotyping as an alternative approach. Phylogenetic analysis of 35 nuclear markers differentiated E. falciformis from house mice from isolates from Apodemus hosts. Isolates of E. vermiformis from Mus are still found in clusters interspersed with non‐Mus isolates, even with this high‐resolution data. In conclusion, we show that species‐level resolution should not be assumed for COI and 18S markers in coccidia. Host–parasite cospeciation at shallow phylogenetic nodes, as well as contemporary coccidian host ranges more generally, is still open questions that need to be addressed using novel genetic markers with higher resolution. |
format |
Article in Journal/Newspaper |
author |
Víctor Hugo Jarquín‐Díaz Alice Balard Anna Mácová Jenny Jost Tabea Roth von Szepesbéla Karin Berktold Steffen Tank Jana Kvičerová Emanuel Heitlinger |
author_facet |
Víctor Hugo Jarquín‐Díaz Alice Balard Anna Mácová Jenny Jost Tabea Roth von Szepesbéla Karin Berktold Steffen Tank Jana Kvičerová Emanuel Heitlinger |
author_sort |
Víctor Hugo Jarquín‐Díaz |
title |
Generalist Eimeria species in rodents: Multilocus analyses indicate inadequate resolution of established markers |
title_short |
Generalist Eimeria species in rodents: Multilocus analyses indicate inadequate resolution of established markers |
title_full |
Generalist Eimeria species in rodents: Multilocus analyses indicate inadequate resolution of established markers |
title_fullStr |
Generalist Eimeria species in rodents: Multilocus analyses indicate inadequate resolution of established markers |
title_full_unstemmed |
Generalist Eimeria species in rodents: Multilocus analyses indicate inadequate resolution of established markers |
title_sort |
generalist eimeria species in rodents: multilocus analyses indicate inadequate resolution of established markers |
publisher |
Wiley |
publishDate |
2020 |
url |
https://doi.org/10.1002/ece3.5992 https://doaj.org/article/6e83fa8454b14bbaad22291f27e1451a |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Ecology and Evolution, Vol 10, Iss 3, Pp 1378-1389 (2020) |
op_relation |
https://doi.org/10.1002/ece3.5992 https://doaj.org/toc/2045-7758 2045-7758 doi:10.1002/ece3.5992 https://doaj.org/article/6e83fa8454b14bbaad22291f27e1451a |
op_doi |
https://doi.org/10.1002/ece3.5992 |
container_title |
Ecology and Evolution |
container_volume |
10 |
container_issue |
3 |
container_start_page |
1378 |
op_container_end_page |
1389 |
_version_ |
1766069407568625664 |