Comparative proteomic profiling of newly acquired, virulent and attenuated Neoparamoeba perurans proteins associated with amoebic gill disease
Abstract The causative agent of amoebic gill disease, Neoparamoeba perurans is reported to lose virulence during prolonged in vitro maintenance. In this study, the impact of prolonged culture on N. perurans virulence and its proteome was investigated. Two isolates, attenuated and virulent, had their...
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ftdoajarticles:oai:doaj.org/article:6e675552ea154bd6aef26e65dfb2e15c 2023-05-15T15:32:28+02:00 Comparative proteomic profiling of newly acquired, virulent and attenuated Neoparamoeba perurans proteins associated with amoebic gill disease Kerrie Ní Dhufaigh Eugene Dillon Natasha Botwright Anita Talbot Ian O’Connor Eugene MacCarthy Orla Slattery 2021-03-01T00:00:00Z https://doi.org/10.1038/s41598-021-85988-8 https://doaj.org/article/6e675552ea154bd6aef26e65dfb2e15c EN eng Nature Portfolio https://doi.org/10.1038/s41598-021-85988-8 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-021-85988-8 2045-2322 https://doaj.org/article/6e675552ea154bd6aef26e65dfb2e15c Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) Medicine R Science Q article 2021 ftdoajarticles https://doi.org/10.1038/s41598-021-85988-8 2022-12-31T13:55:41Z Abstract The causative agent of amoebic gill disease, Neoparamoeba perurans is reported to lose virulence during prolonged in vitro maintenance. In this study, the impact of prolonged culture on N. perurans virulence and its proteome was investigated. Two isolates, attenuated and virulent, had their virulence assessed in an experimental trial using Atlantic salmon smolts and their bacterial community composition was evaluated by 16S rRNA Illumina MiSeq sequencing. Soluble proteins were isolated from three isolates: a newly acquired, virulent and attenuated N. perurans culture. Proteins were analysed using two-dimensional electrophoresis coupled with liquid chromatography tandem mass spectrometry (LC–MS/MS). The challenge trial using naïve smolts confirmed a loss in virulence in the attenuated N. perurans culture. A greater diversity of bacterial communities was found in the microbiome of the virulent isolate in contrast to a reduction in microbial community richness in the attenuated microbiome. A collated proteome database of N. perurans, Amoebozoa and four bacterial genera resulted in 24 proteins differentially expressed between the three cultures. The present LC–MS/MS results indicate protein synthesis, oxidative stress and immunomodulation are upregulated in a newly acquired N. perurans culture and future studies may exploit these protein identifications for therapeutic purposes in infected farmed fish. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Scientific Reports 11 1 |
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Medicine R Science Q Kerrie Ní Dhufaigh Eugene Dillon Natasha Botwright Anita Talbot Ian O’Connor Eugene MacCarthy Orla Slattery Comparative proteomic profiling of newly acquired, virulent and attenuated Neoparamoeba perurans proteins associated with amoebic gill disease |
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Medicine R Science Q |
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Abstract The causative agent of amoebic gill disease, Neoparamoeba perurans is reported to lose virulence during prolonged in vitro maintenance. In this study, the impact of prolonged culture on N. perurans virulence and its proteome was investigated. Two isolates, attenuated and virulent, had their virulence assessed in an experimental trial using Atlantic salmon smolts and their bacterial community composition was evaluated by 16S rRNA Illumina MiSeq sequencing. Soluble proteins were isolated from three isolates: a newly acquired, virulent and attenuated N. perurans culture. Proteins were analysed using two-dimensional electrophoresis coupled with liquid chromatography tandem mass spectrometry (LC–MS/MS). The challenge trial using naïve smolts confirmed a loss in virulence in the attenuated N. perurans culture. A greater diversity of bacterial communities was found in the microbiome of the virulent isolate in contrast to a reduction in microbial community richness in the attenuated microbiome. A collated proteome database of N. perurans, Amoebozoa and four bacterial genera resulted in 24 proteins differentially expressed between the three cultures. The present LC–MS/MS results indicate protein synthesis, oxidative stress and immunomodulation are upregulated in a newly acquired N. perurans culture and future studies may exploit these protein identifications for therapeutic purposes in infected farmed fish. |
format |
Article in Journal/Newspaper |
author |
Kerrie Ní Dhufaigh Eugene Dillon Natasha Botwright Anita Talbot Ian O’Connor Eugene MacCarthy Orla Slattery |
author_facet |
Kerrie Ní Dhufaigh Eugene Dillon Natasha Botwright Anita Talbot Ian O’Connor Eugene MacCarthy Orla Slattery |
author_sort |
Kerrie Ní Dhufaigh |
title |
Comparative proteomic profiling of newly acquired, virulent and attenuated Neoparamoeba perurans proteins associated with amoebic gill disease |
title_short |
Comparative proteomic profiling of newly acquired, virulent and attenuated Neoparamoeba perurans proteins associated with amoebic gill disease |
title_full |
Comparative proteomic profiling of newly acquired, virulent and attenuated Neoparamoeba perurans proteins associated with amoebic gill disease |
title_fullStr |
Comparative proteomic profiling of newly acquired, virulent and attenuated Neoparamoeba perurans proteins associated with amoebic gill disease |
title_full_unstemmed |
Comparative proteomic profiling of newly acquired, virulent and attenuated Neoparamoeba perurans proteins associated with amoebic gill disease |
title_sort |
comparative proteomic profiling of newly acquired, virulent and attenuated neoparamoeba perurans proteins associated with amoebic gill disease |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doi.org/10.1038/s41598-021-85988-8 https://doaj.org/article/6e675552ea154bd6aef26e65dfb2e15c |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
op_relation |
https://doi.org/10.1038/s41598-021-85988-8 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-021-85988-8 2045-2322 https://doaj.org/article/6e675552ea154bd6aef26e65dfb2e15c |
op_doi |
https://doi.org/10.1038/s41598-021-85988-8 |
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Scientific Reports |
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11 |
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1 |
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1766362963089817600 |