Assessment of DNA integrity through MN bioassay of erythrocytes and histopathological changes in Wallago attu and Cirrhinus mirigala in response to freshwater pollution
The aim of this study was to determine the level of contamination and genotoxic impact through micronucleus assay and histopathology in Wallago attu and Cirrhinus mrigala procured from the polluted site of the River Chenab at industrial and sewage waste disposal. The water sample was found viciously...
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ftdoajarticles:oai:doaj.org/article:6e32b4c2e15849e99dcf21f155140e3e 2023-05-15T15:33:26+02:00 Assessment of DNA integrity through MN bioassay of erythrocytes and histopathological changes in Wallago attu and Cirrhinus mirigala in response to freshwater pollution Bilal Hussain Maleeha Fatima K.A. Al-Ghanim F. Al-Misned Shahid Mahboob 2020-01-01T00:00:00Z https://doi.org/10.1016/j.sjbs.2019.09.003 https://doaj.org/article/6e32b4c2e15849e99dcf21f155140e3e EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S1319562X19301652 https://doaj.org/toc/1319-562X 1319-562X doi:10.1016/j.sjbs.2019.09.003 https://doaj.org/article/6e32b4c2e15849e99dcf21f155140e3e Saudi Journal of Biological Sciences, Vol 27, Iss 1, Pp 251-260 (2020) Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.1016/j.sjbs.2019.09.003 2022-12-31T05:38:31Z The aim of this study was to determine the level of contamination and genotoxic impact through micronucleus assay and histopathology in Wallago attu and Cirrhinus mrigala procured from the polluted site of the River Chenab at industrial and sewage waste disposal. The water sample was found viciously contaminated with heavy metals i.e. Ni, Cr, Mn, Co, Pb, Hg, Zn, Sn, Cu while all other physio-chemical variables crossed the suggested limits of WHO. The heavy metals load induced histopathological alterations were correlated to environmental degradation and the productivity of this biological system. W. attu and C. mrigala harvested from contaminated sites of the river indicated higher intensity of DNA damage through micronucleus induction and nuclear abnormalities with 5.46 ± 0.17, 1.23 ± 0.08 and 4.2 ± 0.11, 0.4 ± 0.04‰ respectively. Muscle sections of W. attu and C. mrigala harvested from the polluted section of river demonstrated the necrosis, degeneration of muscle fibers, intra-fibular edema and release of the blood into the tissues due to the bursting of blocked of the blood vessels. Dermal layers showed degeneration of the collagen bundles those were found loose or collapsed in some regions. Photomicrography also revealed vacuolar degeneration in muscle tissues and atrophy of muscle bundles. Intra fibular edema and splitting of muscle fibers were also seen along with bioaccumulation of toxicants. W. attu showed maximum incidence of alterations with highest histopathological alteration index related to environmental degradation. Control fish samples showed normal muscle tissues with normal equally spaced muscle bundles and myotomes. Keywords: Pollution, Xenobiotics, Fish, Micronucleus, Histopathology Article in Journal/Newspaper Attu Directory of Open Access Journals: DOAJ Articles Saudi Journal of Biological Sciences 27 1 251 260 |
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Biology (General) QH301-705.5 |
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Biology (General) QH301-705.5 Bilal Hussain Maleeha Fatima K.A. Al-Ghanim F. Al-Misned Shahid Mahboob Assessment of DNA integrity through MN bioassay of erythrocytes and histopathological changes in Wallago attu and Cirrhinus mirigala in response to freshwater pollution |
topic_facet |
Biology (General) QH301-705.5 |
description |
The aim of this study was to determine the level of contamination and genotoxic impact through micronucleus assay and histopathology in Wallago attu and Cirrhinus mrigala procured from the polluted site of the River Chenab at industrial and sewage waste disposal. The water sample was found viciously contaminated with heavy metals i.e. Ni, Cr, Mn, Co, Pb, Hg, Zn, Sn, Cu while all other physio-chemical variables crossed the suggested limits of WHO. The heavy metals load induced histopathological alterations were correlated to environmental degradation and the productivity of this biological system. W. attu and C. mrigala harvested from contaminated sites of the river indicated higher intensity of DNA damage through micronucleus induction and nuclear abnormalities with 5.46 ± 0.17, 1.23 ± 0.08 and 4.2 ± 0.11, 0.4 ± 0.04‰ respectively. Muscle sections of W. attu and C. mrigala harvested from the polluted section of river demonstrated the necrosis, degeneration of muscle fibers, intra-fibular edema and release of the blood into the tissues due to the bursting of blocked of the blood vessels. Dermal layers showed degeneration of the collagen bundles those were found loose or collapsed in some regions. Photomicrography also revealed vacuolar degeneration in muscle tissues and atrophy of muscle bundles. Intra fibular edema and splitting of muscle fibers were also seen along with bioaccumulation of toxicants. W. attu showed maximum incidence of alterations with highest histopathological alteration index related to environmental degradation. Control fish samples showed normal muscle tissues with normal equally spaced muscle bundles and myotomes. Keywords: Pollution, Xenobiotics, Fish, Micronucleus, Histopathology |
format |
Article in Journal/Newspaper |
author |
Bilal Hussain Maleeha Fatima K.A. Al-Ghanim F. Al-Misned Shahid Mahboob |
author_facet |
Bilal Hussain Maleeha Fatima K.A. Al-Ghanim F. Al-Misned Shahid Mahboob |
author_sort |
Bilal Hussain |
title |
Assessment of DNA integrity through MN bioassay of erythrocytes and histopathological changes in Wallago attu and Cirrhinus mirigala in response to freshwater pollution |
title_short |
Assessment of DNA integrity through MN bioassay of erythrocytes and histopathological changes in Wallago attu and Cirrhinus mirigala in response to freshwater pollution |
title_full |
Assessment of DNA integrity through MN bioassay of erythrocytes and histopathological changes in Wallago attu and Cirrhinus mirigala in response to freshwater pollution |
title_fullStr |
Assessment of DNA integrity through MN bioassay of erythrocytes and histopathological changes in Wallago attu and Cirrhinus mirigala in response to freshwater pollution |
title_full_unstemmed |
Assessment of DNA integrity through MN bioassay of erythrocytes and histopathological changes in Wallago attu and Cirrhinus mirigala in response to freshwater pollution |
title_sort |
assessment of dna integrity through mn bioassay of erythrocytes and histopathological changes in wallago attu and cirrhinus mirigala in response to freshwater pollution |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://doi.org/10.1016/j.sjbs.2019.09.003 https://doaj.org/article/6e32b4c2e15849e99dcf21f155140e3e |
genre |
Attu |
genre_facet |
Attu |
op_source |
Saudi Journal of Biological Sciences, Vol 27, Iss 1, Pp 251-260 (2020) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S1319562X19301652 https://doaj.org/toc/1319-562X 1319-562X doi:10.1016/j.sjbs.2019.09.003 https://doaj.org/article/6e32b4c2e15849e99dcf21f155140e3e |
op_doi |
https://doi.org/10.1016/j.sjbs.2019.09.003 |
container_title |
Saudi Journal of Biological Sciences |
container_volume |
27 |
container_issue |
1 |
container_start_page |
251 |
op_container_end_page |
260 |
_version_ |
1766364010118119424 |