Status of insecticide resistance in Anopheles mosquitoes in Ubon Ratchathani province, Northeastern Thailand
Abstract Background Malaria is common in hilly, forested areas along national borders in Southeast Asia. Insecticide resistance in malaria vectors has been detected in a few countries in the Greater Mekong sub-region (GMS), representing a threat to malaria control and prevention. This study aims to...
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ftdoajarticles:oai:doaj.org/article:6e2bc3be14d1452594bc010479816a79 2023-05-15T15:14:54+02:00 Status of insecticide resistance in Anopheles mosquitoes in Ubon Ratchathani province, Northeastern Thailand Anchana Sumarnrote Hans J. Overgaard Nattapol Marasri Bénédicte Fustec Kanutcharee Thanispong Theeraphap Chareonviriyaphap Vincent Corbel 2017-07-01T00:00:00Z https://doi.org/10.1186/s12936-017-1948-z https://doaj.org/article/6e2bc3be14d1452594bc010479816a79 EN eng BMC http://link.springer.com/article/10.1186/s12936-017-1948-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1948-z 1475-2875 https://doaj.org/article/6e2bc3be14d1452594bc010479816a79 Malaria Journal, Vol 16, Iss 1, Pp 1-13 (2017) Malaria Vectors Anopheles Pyrethroids DDT Resistance Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-1948-z 2022-12-31T13:52:11Z Abstract Background Malaria is common in hilly, forested areas along national borders in Southeast Asia. Insecticide resistance in malaria vectors has been detected in a few countries in the Greater Mekong sub-region (GMS), representing a threat to malaria control and prevention. This study aims to determine the insecticide resistance status of Anopheles mosquitoes in Ubon Ratchathani province, northeastern Thailand, where increasing number of malaria cases were reported recently. Methods Mosquitoes were collected in 2013–2015 using human landing and cattle bait collections in six sites during both the rainy and dry seasons. Mosquitoes were first morphologically identified to species and their susceptibility status to deltamethrin (0.05%), permethrin (0.75%) and DDT (4%) investigated, according to WHO guidelines. Bioassays with the synergists PBO and DEF were carried out to address the role of detoxifying enzymes in insecticide resistance. DNA sequencing of a fragment of the voltage-gated sodium channel gene was carried out to detect knock-down resistance (kdr) substitutions at position 1014 in resistant species. Results Due to low vector abundance, complete bioassays (n ≥ 100 mosquitoes) were only achieved for Anopheles hyrcanus s.l., which was resistant to all insecticides tested (mortality ranged from 45 to 87%). Suspected resistance to DDT was found in Anopheles barbirostris s.l. (mortality 69%), but it was susceptible to deltamethrin (mortality 97–100%) and permethrin (mortality 100%). Although insufficient number of primary vectors were collected, results showed that Anopheles dirus s.l. and Anopheles maculatus s.l. were susceptible to deltamethrin (mortality 100%). Anopheles nivipes and Anopheles philippinensis were susceptible to all three insecticides. PBO significantly increased mortality to deltamethrin and permethrin in pyrethroid-resistant An. hyrcanus s.l. None of the sequenced specimens presented the L1014F or L1014S mutation. Discussion This study shows that insecticide resistance is present in ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
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English |
topic |
Malaria Vectors Anopheles Pyrethroids DDT Resistance Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria Vectors Anopheles Pyrethroids DDT Resistance Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Anchana Sumarnrote Hans J. Overgaard Nattapol Marasri Bénédicte Fustec Kanutcharee Thanispong Theeraphap Chareonviriyaphap Vincent Corbel Status of insecticide resistance in Anopheles mosquitoes in Ubon Ratchathani province, Northeastern Thailand |
topic_facet |
Malaria Vectors Anopheles Pyrethroids DDT Resistance Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria is common in hilly, forested areas along national borders in Southeast Asia. Insecticide resistance in malaria vectors has been detected in a few countries in the Greater Mekong sub-region (GMS), representing a threat to malaria control and prevention. This study aims to determine the insecticide resistance status of Anopheles mosquitoes in Ubon Ratchathani province, northeastern Thailand, where increasing number of malaria cases were reported recently. Methods Mosquitoes were collected in 2013–2015 using human landing and cattle bait collections in six sites during both the rainy and dry seasons. Mosquitoes were first morphologically identified to species and their susceptibility status to deltamethrin (0.05%), permethrin (0.75%) and DDT (4%) investigated, according to WHO guidelines. Bioassays with the synergists PBO and DEF were carried out to address the role of detoxifying enzymes in insecticide resistance. DNA sequencing of a fragment of the voltage-gated sodium channel gene was carried out to detect knock-down resistance (kdr) substitutions at position 1014 in resistant species. Results Due to low vector abundance, complete bioassays (n ≥ 100 mosquitoes) were only achieved for Anopheles hyrcanus s.l., which was resistant to all insecticides tested (mortality ranged from 45 to 87%). Suspected resistance to DDT was found in Anopheles barbirostris s.l. (mortality 69%), but it was susceptible to deltamethrin (mortality 97–100%) and permethrin (mortality 100%). Although insufficient number of primary vectors were collected, results showed that Anopheles dirus s.l. and Anopheles maculatus s.l. were susceptible to deltamethrin (mortality 100%). Anopheles nivipes and Anopheles philippinensis were susceptible to all three insecticides. PBO significantly increased mortality to deltamethrin and permethrin in pyrethroid-resistant An. hyrcanus s.l. None of the sequenced specimens presented the L1014F or L1014S mutation. Discussion This study shows that insecticide resistance is present in ... |
format |
Article in Journal/Newspaper |
author |
Anchana Sumarnrote Hans J. Overgaard Nattapol Marasri Bénédicte Fustec Kanutcharee Thanispong Theeraphap Chareonviriyaphap Vincent Corbel |
author_facet |
Anchana Sumarnrote Hans J. Overgaard Nattapol Marasri Bénédicte Fustec Kanutcharee Thanispong Theeraphap Chareonviriyaphap Vincent Corbel |
author_sort |
Anchana Sumarnrote |
title |
Status of insecticide resistance in Anopheles mosquitoes in Ubon Ratchathani province, Northeastern Thailand |
title_short |
Status of insecticide resistance in Anopheles mosquitoes in Ubon Ratchathani province, Northeastern Thailand |
title_full |
Status of insecticide resistance in Anopheles mosquitoes in Ubon Ratchathani province, Northeastern Thailand |
title_fullStr |
Status of insecticide resistance in Anopheles mosquitoes in Ubon Ratchathani province, Northeastern Thailand |
title_full_unstemmed |
Status of insecticide resistance in Anopheles mosquitoes in Ubon Ratchathani province, Northeastern Thailand |
title_sort |
status of insecticide resistance in anopheles mosquitoes in ubon ratchathani province, northeastern thailand |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-1948-z https://doaj.org/article/6e2bc3be14d1452594bc010479816a79 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-13 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-1948-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1948-z 1475-2875 https://doaj.org/article/6e2bc3be14d1452594bc010479816a79 |
op_doi |
https://doi.org/10.1186/s12936-017-1948-z |
container_title |
Malaria Journal |
container_volume |
16 |
container_issue |
1 |
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1766345302445391872 |