Haplotypic analysis of cox1 from Toxocara canis demonstrates five distinct clades that are not geographically defined.
Background Toxocara canis is a cosmopolitan parasite of dogs that is transmitted transplacentally to puppies resulting in widespread shedding of eggs in the environment. However, it is not clear if there are dominant parasite genotypes that are more common, pathogenic, or likely to be zoonotic. Meth...
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ftdoajarticles:oai:doaj.org/article:6df935771d054bb7935f04878f6baa23 2023-11-12T04:13:18+01:00 Haplotypic analysis of cox1 from Toxocara canis demonstrates five distinct clades that are not geographically defined. Katy A Martin Jeba R J Jesudoss Chelladurai Abrha Bsrat Cassan Pulaski Alice C Y Lee Lindsay A Starkey Matthew T Brewer 2023-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011665 https://doaj.org/article/6df935771d054bb7935f04878f6baa23 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011665 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011665 https://doaj.org/article/6df935771d054bb7935f04878f6baa23 PLoS Neglected Tropical Diseases, Vol 17, Iss 10, p e0011665 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011665 2023-10-29T00:35:08Z Background Toxocara canis is a cosmopolitan parasite of dogs that is transmitted transplacentally to puppies resulting in widespread shedding of eggs in the environment. However, it is not clear if there are dominant parasite genotypes that are more common, pathogenic, or likely to be zoonotic. Methods/principle findings Sequences of mitochondrial cox1 gene from adult worms were used to compare parasites from the United States with submitted sequences from parasites isolated from dogs in different countries. Our analysis revealed at least 55 haplotypes. While we expected the North American worms to form a distinct cluster, we found haplotypes of T. canis reported elsewhere existing in this population. Interestingly, combining the sequence data from our study with the available GenBank data, analysis of cox1 sequences results in five distinct clades that are not geographically defined. Conclusions The five clades of T. canis revealed in this study potentially have unique life histories, traits, or host preferences. Additional investigation is needed to see if these distinct clades represent cryptic species with clinically useful attributes or genotypes with taxonomic value. Evaluation of common mitochondrial genes may reveal distinct populations of zoonotic T. canis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles PLOS Neglected Tropical Diseases 17 10 e0011665 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Katy A Martin Jeba R J Jesudoss Chelladurai Abrha Bsrat Cassan Pulaski Alice C Y Lee Lindsay A Starkey Matthew T Brewer Haplotypic analysis of cox1 from Toxocara canis demonstrates five distinct clades that are not geographically defined. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Toxocara canis is a cosmopolitan parasite of dogs that is transmitted transplacentally to puppies resulting in widespread shedding of eggs in the environment. However, it is not clear if there are dominant parasite genotypes that are more common, pathogenic, or likely to be zoonotic. Methods/principle findings Sequences of mitochondrial cox1 gene from adult worms were used to compare parasites from the United States with submitted sequences from parasites isolated from dogs in different countries. Our analysis revealed at least 55 haplotypes. While we expected the North American worms to form a distinct cluster, we found haplotypes of T. canis reported elsewhere existing in this population. Interestingly, combining the sequence data from our study with the available GenBank data, analysis of cox1 sequences results in five distinct clades that are not geographically defined. Conclusions The five clades of T. canis revealed in this study potentially have unique life histories, traits, or host preferences. Additional investigation is needed to see if these distinct clades represent cryptic species with clinically useful attributes or genotypes with taxonomic value. Evaluation of common mitochondrial genes may reveal distinct populations of zoonotic T. canis. |
format |
Article in Journal/Newspaper |
author |
Katy A Martin Jeba R J Jesudoss Chelladurai Abrha Bsrat Cassan Pulaski Alice C Y Lee Lindsay A Starkey Matthew T Brewer |
author_facet |
Katy A Martin Jeba R J Jesudoss Chelladurai Abrha Bsrat Cassan Pulaski Alice C Y Lee Lindsay A Starkey Matthew T Brewer |
author_sort |
Katy A Martin |
title |
Haplotypic analysis of cox1 from Toxocara canis demonstrates five distinct clades that are not geographically defined. |
title_short |
Haplotypic analysis of cox1 from Toxocara canis demonstrates five distinct clades that are not geographically defined. |
title_full |
Haplotypic analysis of cox1 from Toxocara canis demonstrates five distinct clades that are not geographically defined. |
title_fullStr |
Haplotypic analysis of cox1 from Toxocara canis demonstrates five distinct clades that are not geographically defined. |
title_full_unstemmed |
Haplotypic analysis of cox1 from Toxocara canis demonstrates five distinct clades that are not geographically defined. |
title_sort |
haplotypic analysis of cox1 from toxocara canis demonstrates five distinct clades that are not geographically defined. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011665 https://doaj.org/article/6df935771d054bb7935f04878f6baa23 |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 10, p e0011665 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011665 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011665 https://doaj.org/article/6df935771d054bb7935f04878f6baa23 |
op_doi |
https://doi.org/10.1371/journal.pntd.0011665 |
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PLOS Neglected Tropical Diseases |
container_volume |
17 |
container_issue |
10 |
container_start_page |
e0011665 |
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1782331368419622912 |