Regulation of adductor muscle growth by the IGF-1/AKT pathway in the triploid Pacific oyster, Crassostrea gigas

• Published in: Fisheries and Aquatic Sciences
• Main Authors: Eun-Young Kim, Youn Hee Choi
• Format: Article in Journal/Newspaper
• Language: English
• Published: The Korean Society of Fisheries and Aquatic Science 2019
• Subjects: Triploid oyster; Adductor muscle; Muscle growth; Protein synthesis; Protein degradation; Aquaculture. Fisheries. Angling; SH1-691; Pacific; Crassostrea gigas; Pacific oyster
• Online Access: https://doi.org/10.1186/s41240-019-0134-3; https://doaj.org/article/6d8c588ad91e40caa52fc14dd5aad714

Abstract We investigated the insulin-like growth factor 1 (IGF-1)/AKT signaling pathway involved in muscle formation, growth, and movement in the adductor muscle of triploid Pacific oyster, Crassostrea gigas. Large and small triploid oysters (LTs and STs) cultured under identical conditions were screened, and the signaling pathways of individuals with superior growth were compared and analyzed. mRNA and protein expression levels of actin, troponin, tropomyosin, and myosin, proteins important in muscle formation, were higher in LTs compared with STs. Expression levels of IGF-1, IGF binding protein (IGFBP), and IGFBP complex acid-labile subunit were also higher in LTs compared with STs. Phosphorylation of the IGF receptor as well as that of AKT was high in LTs. In addition, the expression of phospho-mammalian target of rapamycin and phospho-glycogen synthase kinase 3β was increased and the expression of Forkhead box O3 was decreased in LTs. Therefore, we suggested that the IGF-1/AKT signaling pathway affects the formation, growth, and movement of the adductor muscle in triploid oysters.