Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains
Pseudomonas aeruginosa infections cause significant mortality and morbidity in health care settings. Strategies to prevent and control the emergence and spread of P. aeruginosa within hospitals involve implementation of barrier methods and antimicrobial stewardship programs. However, there is still...
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ftdoajarticles:oai:doaj.org/article:6d82fd11e1af46648a7790e675a9e6b4 2023-05-15T15:08:12+02:00 Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains CMCB Fortaleza CE Bacchi DE Oliveira MC Ramos 2011-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992011000200008 https://doaj.org/article/6d82fd11e1af46648a7790e675a9e6b4 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200008 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000200008 1678-9199 https://doaj.org/article/6d82fd11e1af46648a7790e675a9e6b4 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 2, Pp 176-183 (2011) Pseudomonas aeruginosa strain typing ERIC-PCR nosocomial infections health care infections Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2011 ftdoajarticles https://doi.org/10.1590/S1678-91992011000200008 2022-12-31T08:49:09Z Pseudomonas aeruginosa infections cause significant mortality and morbidity in health care settings. Strategies to prevent and control the emergence and spread of P. aeruginosa within hospitals involve implementation of barrier methods and antimicrobial stewardship programs. However, there is still much debate over which of these measures holds the utmost importance. Molecular strain typing may help elucidate this issue. In our study, 71 nosocomial isolates from 41 patients and 23 community-acquired isolates from 21 patients were genotyped. Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) was performed. Band patterns were compared using similarity coefficients of Dice, Jaccard and simple matching. Strain similarity for nosocomial strains varied from 0.14 to 1.00 (Dice); 0.08 to 1.00 (Jaccard) and 0.58 to 1.00 (simple matching). Forty patterns were identified. In most units, several clones coexisted. However, there was evidence of clonal dissemination in the high risk nursery, neurology and two surgical units. Each and every community-acquired strain produced a unique distinct pattern. Results suggest that cross transmission of P. aeruginosa was an uncommon event in our hospital. This points out to a minor role for barrier methods in the control of P. aeruginosa spread. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 17 2 176 183 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Pseudomonas aeruginosa strain typing ERIC-PCR nosocomial infections health care infections Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Pseudomonas aeruginosa strain typing ERIC-PCR nosocomial infections health care infections Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 CMCB Fortaleza CE Bacchi DE Oliveira MC Ramos Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains |
topic_facet |
Pseudomonas aeruginosa strain typing ERIC-PCR nosocomial infections health care infections Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Pseudomonas aeruginosa infections cause significant mortality and morbidity in health care settings. Strategies to prevent and control the emergence and spread of P. aeruginosa within hospitals involve implementation of barrier methods and antimicrobial stewardship programs. However, there is still much debate over which of these measures holds the utmost importance. Molecular strain typing may help elucidate this issue. In our study, 71 nosocomial isolates from 41 patients and 23 community-acquired isolates from 21 patients were genotyped. Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) was performed. Band patterns were compared using similarity coefficients of Dice, Jaccard and simple matching. Strain similarity for nosocomial strains varied from 0.14 to 1.00 (Dice); 0.08 to 1.00 (Jaccard) and 0.58 to 1.00 (simple matching). Forty patterns were identified. In most units, several clones coexisted. However, there was evidence of clonal dissemination in the high risk nursery, neurology and two surgical units. Each and every community-acquired strain produced a unique distinct pattern. Results suggest that cross transmission of P. aeruginosa was an uncommon event in our hospital. This points out to a minor role for barrier methods in the control of P. aeruginosa spread. |
format |
Article in Journal/Newspaper |
author |
CMCB Fortaleza CE Bacchi DE Oliveira MC Ramos |
author_facet |
CMCB Fortaleza CE Bacchi DE Oliveira MC Ramos |
author_sort |
CMCB Fortaleza |
title |
Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains |
title_short |
Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains |
title_full |
Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains |
title_fullStr |
Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains |
title_full_unstemmed |
Polyclonal endemicity of Pseudomonas aeruginosa in a teaching hospital from Brazil: molecular typing of decade-old strains |
title_sort |
polyclonal endemicity of pseudomonas aeruginosa in a teaching hospital from brazil: molecular typing of decade-old strains |
publisher |
SciELO |
publishDate |
2011 |
url |
https://doi.org/10.1590/S1678-91992011000200008 https://doaj.org/article/6d82fd11e1af46648a7790e675a9e6b4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 2, Pp 176-183 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200008 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000200008 1678-9199 https://doaj.org/article/6d82fd11e1af46648a7790e675a9e6b4 |
op_doi |
https://doi.org/10.1590/S1678-91992011000200008 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
17 |
container_issue |
2 |
container_start_page |
176 |
op_container_end_page |
183 |
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