Identification of a conserved var gene in different Plasmodium falciparum strains
Abstract Background The multicopy var gene family of Plasmodium falciparum is of crucial importance for pathogenesis and antigenic variation. So far only var2csa, the var gene responsible for placental malaria, was found to be highly conserved among all P. falciparum strains. Here, a new conserved 3...
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ftdoajarticles:oai:doaj.org/article:6d73f5c0de4e4f4d8240455540d44f76 2023-05-15T15:16:05+02:00 Identification of a conserved var gene in different Plasmodium falciparum strains Sandra Dimonte Ellen I. Bruske Corinna Enderes Thomas D. Otto Louise Turner Peter Kremsner Matthias Frank 2020-05-01T00:00:00Z https://doi.org/10.1186/s12936-020-03257-x https://doaj.org/article/6d73f5c0de4e4f4d8240455540d44f76 EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03257-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03257-x 1475-2875 https://doaj.org/article/6d73f5c0de4e4f4d8240455540d44f76 Malaria Journal, Vol 19, Iss 1, Pp 1-15 (2020) Malaria var genes Genetic diversity Microsatellites Recombination VSA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03257-x 2022-12-31T03:56:22Z Abstract Background The multicopy var gene family of Plasmodium falciparum is of crucial importance for pathogenesis and antigenic variation. So far only var2csa, the var gene responsible for placental malaria, was found to be highly conserved among all P. falciparum strains. Here, a new conserved 3D7 var gene (PF3D7_0617400) is identified in several field isolates. Methods DNA sequencing, transcriptional analysis, Cluster of Differentiation (CD) 36-receptor binding, indirect immunofluorescence with PF3D7_0617400-antibodies and quantification of surface reactivity against semi-immune sera were used to characterize an NF54 clone and a Gabonese field isolate clone (MOA C3) transcribing the gene. A population of 714 whole genome sequenced parasites was analysed to characterize the conservation of the locus in African and Asian isolates. The genetic diversity of two var2csa fragments was compared with the genetic diversity of 57 microsatellites fragments in field isolates. Results PFGA01_060022400 was identified in a Gabonese parasite isolate (MOA) from a chronic infection and found to be 99% identical with PF3D7_0617400 of the 3D7 genome strain. Transcriptional analysis and immunofluorescence showed expression of the gene in an NF54 and a MOA clone but CD36 binding assays and surface reactivity to semi-immune sera differed markedly in the two clones. Long-read Pacific bioscience whole genome sequencing showed that PFGA01_060022400 is located in the internal cluster of chromosome 6. The full length PFGA01_060022400 was detected in 36 of 714 P. falciparum isolates and 500 bp fragments were identified in more than 100 isolates. var2csa was in parts highly conserved (He = 0) but in other parts as variable (He = 0.86) as the 57 microsatellites markers (He = 0.8). Conclusions Individual var gene sequences exhibit conservation in the global parasite population suggesting that purifying selection may limit overall genetic diversity of some var genes. Notably, field and laboratory isolates expressing the same var gene ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Moa ENVELOPE(15.184,15.184,67.286,67.286) Pacific Malaria Journal 19 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria var genes Genetic diversity Microsatellites Recombination VSA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria var genes Genetic diversity Microsatellites Recombination VSA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Sandra Dimonte Ellen I. Bruske Corinna Enderes Thomas D. Otto Louise Turner Peter Kremsner Matthias Frank Identification of a conserved var gene in different Plasmodium falciparum strains |
topic_facet |
Malaria var genes Genetic diversity Microsatellites Recombination VSA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The multicopy var gene family of Plasmodium falciparum is of crucial importance for pathogenesis and antigenic variation. So far only var2csa, the var gene responsible for placental malaria, was found to be highly conserved among all P. falciparum strains. Here, a new conserved 3D7 var gene (PF3D7_0617400) is identified in several field isolates. Methods DNA sequencing, transcriptional analysis, Cluster of Differentiation (CD) 36-receptor binding, indirect immunofluorescence with PF3D7_0617400-antibodies and quantification of surface reactivity against semi-immune sera were used to characterize an NF54 clone and a Gabonese field isolate clone (MOA C3) transcribing the gene. A population of 714 whole genome sequenced parasites was analysed to characterize the conservation of the locus in African and Asian isolates. The genetic diversity of two var2csa fragments was compared with the genetic diversity of 57 microsatellites fragments in field isolates. Results PFGA01_060022400 was identified in a Gabonese parasite isolate (MOA) from a chronic infection and found to be 99% identical with PF3D7_0617400 of the 3D7 genome strain. Transcriptional analysis and immunofluorescence showed expression of the gene in an NF54 and a MOA clone but CD36 binding assays and surface reactivity to semi-immune sera differed markedly in the two clones. Long-read Pacific bioscience whole genome sequencing showed that PFGA01_060022400 is located in the internal cluster of chromosome 6. The full length PFGA01_060022400 was detected in 36 of 714 P. falciparum isolates and 500 bp fragments were identified in more than 100 isolates. var2csa was in parts highly conserved (He = 0) but in other parts as variable (He = 0.86) as the 57 microsatellites markers (He = 0.8). Conclusions Individual var gene sequences exhibit conservation in the global parasite population suggesting that purifying selection may limit overall genetic diversity of some var genes. Notably, field and laboratory isolates expressing the same var gene ... |
format |
Article in Journal/Newspaper |
author |
Sandra Dimonte Ellen I. Bruske Corinna Enderes Thomas D. Otto Louise Turner Peter Kremsner Matthias Frank |
author_facet |
Sandra Dimonte Ellen I. Bruske Corinna Enderes Thomas D. Otto Louise Turner Peter Kremsner Matthias Frank |
author_sort |
Sandra Dimonte |
title |
Identification of a conserved var gene in different Plasmodium falciparum strains |
title_short |
Identification of a conserved var gene in different Plasmodium falciparum strains |
title_full |
Identification of a conserved var gene in different Plasmodium falciparum strains |
title_fullStr |
Identification of a conserved var gene in different Plasmodium falciparum strains |
title_full_unstemmed |
Identification of a conserved var gene in different Plasmodium falciparum strains |
title_sort |
identification of a conserved var gene in different plasmodium falciparum strains |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03257-x https://doaj.org/article/6d73f5c0de4e4f4d8240455540d44f76 |
long_lat |
ENVELOPE(15.184,15.184,67.286,67.286) |
geographic |
Arctic Moa Pacific |
geographic_facet |
Arctic Moa Pacific |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-15 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03257-x https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03257-x 1475-2875 https://doaj.org/article/6d73f5c0de4e4f4d8240455540d44f76 |
op_doi |
https://doi.org/10.1186/s12936-020-03257-x |
container_title |
Malaria Journal |
container_volume |
19 |
container_issue |
1 |
_version_ |
1766346399675318272 |