Kinetic Analysis of the Lipase-Catalyzed Hydrolysis of Erythritol and Pentaerythritol Fatty Acid Esters: A Biotechnological Application for Making Low-Calorie Healthy Food Alternatives
Contemporary consumers demand healthier and more nourishing food, and thus, alternative foods that are low-calorie in fats and/or sugars are preferred. These desired properties may be attained by substituting the fatty acid esters of erythritol and pentaerythritol due to their antioxidant action and...
Published in: | Catalysts |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3390/catal10090965 https://doaj.org/article/6d6919c1ab5943cb870266b3496c4c92 |
id |
ftdoajarticles:oai:doaj.org/article:6d6919c1ab5943cb870266b3496c4c92 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:6d6919c1ab5943cb870266b3496c4c92 2023-05-15T14:03:17+02:00 Kinetic Analysis of the Lipase-Catalyzed Hydrolysis of Erythritol and Pentaerythritol Fatty Acid Esters: A Biotechnological Application for Making Low-Calorie Healthy Food Alternatives Olga A. Gkini Panagiota-Yiolanda Stergiou Athanasios Foukis Panayiotis V. Ioannou Emmanuel M. Papamichael 2020-08-01T00:00:00Z https://doi.org/10.3390/catal10090965 https://doaj.org/article/6d6919c1ab5943cb870266b3496c4c92 EN eng MDPI AG https://www.mdpi.com/2073-4344/10/9/965 https://doaj.org/toc/2073-4344 doi:10.3390/catal10090965 2073-4344 https://doaj.org/article/6d6919c1ab5943cb870266b3496c4c92 Catalysts, Vol 10, Iss 965, p 965 (2020) erythritol pentaerythritol tetraesters fatty acids Candida antarctica lipase-B low calorie dietary foods Chemical technology TP1-1185 Chemistry QD1-999 article 2020 ftdoajarticles https://doi.org/10.3390/catal10090965 2022-12-31T11:28:58Z Contemporary consumers demand healthier and more nourishing food, and thus, alternative foods that are low-calorie in fats and/or sugars are preferred. These desired properties may be attained by substituting the fatty acid esters of erythritol and pentaerythritol due to their antioxidant action and low toxicity for humans. In this work, the catalyzed hydrolysis of five fatty acid tetraesters of erythritol and/or pentaerythritol by both porcine pancreas type VI-s lipase (PPL) and Candida antarctica lipase-B (CALB) were studied kinetically. In all cases, except the hydrolysis of pentaerythritol tetrastearate by CALB, Michaelis–Menten kinetics were observed. In addition, the p K a values of the fatty acids released due to the catalyzed hydrolysis of the studied tetraesters by CALB were estimated. In the course of the aforementioned procedures, it was found that the CALB-catalyzed hydrolysis was incomplete to various degrees among four of the five studied tetraesters (excluding erythritol tetraoleate), and one or more estimated apparent p K a values were obtained. These results are novel, and by means of applied methodology, they reveal that erythritol and/or pentaerythritol tetraesters of medium- and long-chain fatty acids are suitable candidates for use as beneficial alternatives to butter and/or sweeteners. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Catalysts 10 9 965 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
erythritol pentaerythritol tetraesters fatty acids Candida antarctica lipase-B low calorie dietary foods Chemical technology TP1-1185 Chemistry QD1-999 |
spellingShingle |
erythritol pentaerythritol tetraesters fatty acids Candida antarctica lipase-B low calorie dietary foods Chemical technology TP1-1185 Chemistry QD1-999 Olga A. Gkini Panagiota-Yiolanda Stergiou Athanasios Foukis Panayiotis V. Ioannou Emmanuel M. Papamichael Kinetic Analysis of the Lipase-Catalyzed Hydrolysis of Erythritol and Pentaerythritol Fatty Acid Esters: A Biotechnological Application for Making Low-Calorie Healthy Food Alternatives |
topic_facet |
erythritol pentaerythritol tetraesters fatty acids Candida antarctica lipase-B low calorie dietary foods Chemical technology TP1-1185 Chemistry QD1-999 |
description |
Contemporary consumers demand healthier and more nourishing food, and thus, alternative foods that are low-calorie in fats and/or sugars are preferred. These desired properties may be attained by substituting the fatty acid esters of erythritol and pentaerythritol due to their antioxidant action and low toxicity for humans. In this work, the catalyzed hydrolysis of five fatty acid tetraesters of erythritol and/or pentaerythritol by both porcine pancreas type VI-s lipase (PPL) and Candida antarctica lipase-B (CALB) were studied kinetically. In all cases, except the hydrolysis of pentaerythritol tetrastearate by CALB, Michaelis–Menten kinetics were observed. In addition, the p K a values of the fatty acids released due to the catalyzed hydrolysis of the studied tetraesters by CALB were estimated. In the course of the aforementioned procedures, it was found that the CALB-catalyzed hydrolysis was incomplete to various degrees among four of the five studied tetraesters (excluding erythritol tetraoleate), and one or more estimated apparent p K a values were obtained. These results are novel, and by means of applied methodology, they reveal that erythritol and/or pentaerythritol tetraesters of medium- and long-chain fatty acids are suitable candidates for use as beneficial alternatives to butter and/or sweeteners. |
format |
Article in Journal/Newspaper |
author |
Olga A. Gkini Panagiota-Yiolanda Stergiou Athanasios Foukis Panayiotis V. Ioannou Emmanuel M. Papamichael |
author_facet |
Olga A. Gkini Panagiota-Yiolanda Stergiou Athanasios Foukis Panayiotis V. Ioannou Emmanuel M. Papamichael |
author_sort |
Olga A. Gkini |
title |
Kinetic Analysis of the Lipase-Catalyzed Hydrolysis of Erythritol and Pentaerythritol Fatty Acid Esters: A Biotechnological Application for Making Low-Calorie Healthy Food Alternatives |
title_short |
Kinetic Analysis of the Lipase-Catalyzed Hydrolysis of Erythritol and Pentaerythritol Fatty Acid Esters: A Biotechnological Application for Making Low-Calorie Healthy Food Alternatives |
title_full |
Kinetic Analysis of the Lipase-Catalyzed Hydrolysis of Erythritol and Pentaerythritol Fatty Acid Esters: A Biotechnological Application for Making Low-Calorie Healthy Food Alternatives |
title_fullStr |
Kinetic Analysis of the Lipase-Catalyzed Hydrolysis of Erythritol and Pentaerythritol Fatty Acid Esters: A Biotechnological Application for Making Low-Calorie Healthy Food Alternatives |
title_full_unstemmed |
Kinetic Analysis of the Lipase-Catalyzed Hydrolysis of Erythritol and Pentaerythritol Fatty Acid Esters: A Biotechnological Application for Making Low-Calorie Healthy Food Alternatives |
title_sort |
kinetic analysis of the lipase-catalyzed hydrolysis of erythritol and pentaerythritol fatty acid esters: a biotechnological application for making low-calorie healthy food alternatives |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/catal10090965 https://doaj.org/article/6d6919c1ab5943cb870266b3496c4c92 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Catalysts, Vol 10, Iss 965, p 965 (2020) |
op_relation |
https://www.mdpi.com/2073-4344/10/9/965 https://doaj.org/toc/2073-4344 doi:10.3390/catal10090965 2073-4344 https://doaj.org/article/6d6919c1ab5943cb870266b3496c4c92 |
op_doi |
https://doi.org/10.3390/catal10090965 |
container_title |
Catalysts |
container_volume |
10 |
container_issue |
9 |
container_start_page |
965 |
_version_ |
1766273912359878656 |