Antibodies elicited in adults by a primary Plasmodium falciparum blood-stage infection recognize different epitopes compared with immune individuals
Abstract Background Asexual stage antibody responses following initial Plasmodium falciparum infections in previously healthy adults may inform vaccine development, yet these have not been as intensively studied as they have in populations from malaria-endemic areas. Methods Serum samples were colle...
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ftdoajarticles:oai:doaj.org/article:6d5ca167753040bfa22e78be29f24ddf 2023-05-15T15:11:53+02:00 Antibodies elicited in adults by a primary Plasmodium falciparum blood-stage infection recognize different epitopes compared with immune individuals Murhandarwati E Elsa H Jouin Helene Wang Lina Eisen Damon P Black Casilda G Mercereau-Puijalon Odile Coppel Ross L 2007-07-01T00:00:00Z https://doi.org/10.1186/1475-2875-6-86 https://doaj.org/article/6d5ca167753040bfa22e78be29f24ddf EN eng BMC http://www.malariajournal.com/content/6/1/86 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-6-86 1475-2875 https://doaj.org/article/6d5ca167753040bfa22e78be29f24ddf Malaria Journal, Vol 6, Iss 1, p 86 (2007) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2007 ftdoajarticles https://doi.org/10.1186/1475-2875-6-86 2022-12-31T10:23:23Z Abstract Background Asexual stage antibody responses following initial Plasmodium falciparum infections in previously healthy adults may inform vaccine development, yet these have not been as intensively studied as they have in populations from malaria-endemic areas. Methods Serum samples were collected over a six-month period from twenty travellers having returned with falciparum malaria. Fourteen of these were malaria-naïve and six had a past history of one to two episodes of malaria. Antibodies to seven asexual stage P. falciparum antigens were measured by ELISA. Invasion inhibitory antibody responses to the 19kDa fragment of merozoite surface protein 1 (MSP1 19 ) were determined. Results Short-lived antibody responses were found in the majority of the subjects. While MSP1 19 antibodies were most common, MSP1 block 2 antibodies were significantly less frequent and recognized conserved domains. Antibodies to MSP2 cross-reacted to the dimorphic allelic families and anti-MSP2 isotypes were not IgG3 skewed as shown previously. MSP1 19 invasion inhibiting antibodies were present in 9/20 patients. A past history of malaria did not influence the frequency of these short-lived, functional antibodies (p = 0.2, 2-tailed Fisher's exact test). Conclusion Adults infected with P. falciparum for the first time, develop relatively short-lived immune responses that, in the case of MSP1 19 , are functional. Antibodies to the polymorphic antigens studied were particularly directed to allelic family specific, non-repetitive and conserved determinants and were not IgG subclass skewed. These responses are substantially different to those found in malaria immune individuals. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 6 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Murhandarwati E Elsa H Jouin Helene Wang Lina Eisen Damon P Black Casilda G Mercereau-Puijalon Odile Coppel Ross L Antibodies elicited in adults by a primary Plasmodium falciparum blood-stage infection recognize different epitopes compared with immune individuals |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Asexual stage antibody responses following initial Plasmodium falciparum infections in previously healthy adults may inform vaccine development, yet these have not been as intensively studied as they have in populations from malaria-endemic areas. Methods Serum samples were collected over a six-month period from twenty travellers having returned with falciparum malaria. Fourteen of these were malaria-naïve and six had a past history of one to two episodes of malaria. Antibodies to seven asexual stage P. falciparum antigens were measured by ELISA. Invasion inhibitory antibody responses to the 19kDa fragment of merozoite surface protein 1 (MSP1 19 ) were determined. Results Short-lived antibody responses were found in the majority of the subjects. While MSP1 19 antibodies were most common, MSP1 block 2 antibodies were significantly less frequent and recognized conserved domains. Antibodies to MSP2 cross-reacted to the dimorphic allelic families and anti-MSP2 isotypes were not IgG3 skewed as shown previously. MSP1 19 invasion inhibiting antibodies were present in 9/20 patients. A past history of malaria did not influence the frequency of these short-lived, functional antibodies (p = 0.2, 2-tailed Fisher's exact test). Conclusion Adults infected with P. falciparum for the first time, develop relatively short-lived immune responses that, in the case of MSP1 19 , are functional. Antibodies to the polymorphic antigens studied were particularly directed to allelic family specific, non-repetitive and conserved determinants and were not IgG subclass skewed. These responses are substantially different to those found in malaria immune individuals. |
format |
Article in Journal/Newspaper |
author |
Murhandarwati E Elsa H Jouin Helene Wang Lina Eisen Damon P Black Casilda G Mercereau-Puijalon Odile Coppel Ross L |
author_facet |
Murhandarwati E Elsa H Jouin Helene Wang Lina Eisen Damon P Black Casilda G Mercereau-Puijalon Odile Coppel Ross L |
author_sort |
Murhandarwati E Elsa H |
title |
Antibodies elicited in adults by a primary Plasmodium falciparum blood-stage infection recognize different epitopes compared with immune individuals |
title_short |
Antibodies elicited in adults by a primary Plasmodium falciparum blood-stage infection recognize different epitopes compared with immune individuals |
title_full |
Antibodies elicited in adults by a primary Plasmodium falciparum blood-stage infection recognize different epitopes compared with immune individuals |
title_fullStr |
Antibodies elicited in adults by a primary Plasmodium falciparum blood-stage infection recognize different epitopes compared with immune individuals |
title_full_unstemmed |
Antibodies elicited in adults by a primary Plasmodium falciparum blood-stage infection recognize different epitopes compared with immune individuals |
title_sort |
antibodies elicited in adults by a primary plasmodium falciparum blood-stage infection recognize different epitopes compared with immune individuals |
publisher |
BMC |
publishDate |
2007 |
url |
https://doi.org/10.1186/1475-2875-6-86 https://doaj.org/article/6d5ca167753040bfa22e78be29f24ddf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 6, Iss 1, p 86 (2007) |
op_relation |
http://www.malariajournal.com/content/6/1/86 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-6-86 1475-2875 https://doaj.org/article/6d5ca167753040bfa22e78be29f24ddf |
op_doi |
https://doi.org/10.1186/1475-2875-6-86 |
container_title |
Malaria Journal |
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6 |
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1 |
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1766342667486101504 |