Mitochondrial activity disruption and local muscle damage induced in mice by Scolopendra polymorpha venom
Abstract Background: Scolopendra polymorpha (S. polymorpha) is a predatory centipede whose venom contains a multiplicity of biochemical effectors that can cause muscle damage and cumulative cell destruction in its prey. Despite previous investigations of S. polymorpha and other centipede venoms, the...
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ftdoajarticles:oai:doaj.org/article:6d27f7fdfb094f1e96a76de1410cd3af 2023-05-15T15:11:12+02:00 Mitochondrial activity disruption and local muscle damage induced in mice by Scolopendra polymorpha venom Judith Tabullo De Robles Francisca Fernández Valverde Lucero Valladares Cisneros Juana Hernández Villeda Ayixon Sánchez-Reyes María del Carmen Gutiérrez https://doi.org/10.1590/1678-9199-jvatitd-2019-0079 https://doaj.org/article/6d27f7fdfb094f1e96a76de1410cd3af EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100316&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0079 https://doaj.org/article/6d27f7fdfb094f1e96a76de1410cd3af Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 centipede venom myotoxic activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2019-0079 2022-12-31T05:28:29Z Abstract Background: Scolopendra polymorpha (S. polymorpha) is a predatory centipede whose venom contains a multiplicity of biochemical effectors that can cause muscle damage and cumulative cell destruction in its prey. Despite previous investigations of S. polymorpha and other centipede venoms, there is a lack of information on the morphological and biochemical patterns elicited by their myotoxic effects. To elucidate these processes, this paper presents evidence of skeletal muscle damage, and alterations in key biochemical mediators that appear only after exposure to centipede venom. Methods: Venom was collected and fractionated using RP-HPLC; mouse extensor digitorum longus (EDL) muscle was exposed to whole venom and venom fractions to evaluate myotoxicity by means of creatine kinase (CK) - a muscle damage marker - activity measurements and histochemical analysis. Results: CK activity was higher in EDL muscle exposed to venom than in unexposed muscle. This increase was observed after 15 min of venom incubation, and remained stable up to 45 min. Venom-exposed EDL muscle showed signs of muscle damage including necrosis, loss of fascicular structure as well as mitochondrial accumulations and ragged red fibers (RRF), suggesting an impairment in the normal mitochondrial arrangement. Nicotinamide adenine dinucleotide (NADH) and cytochrome oxidase (COX) tests also indicate that respiratory complexes might be affected. Conclusion: Our results suggest a different biochemical composition of S. polymorpha venom, based on the different effects of four venom fractions on the cells tested, according to statistical evidence. Fractions F6 and F7 caused the most important alterations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 26 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
centipede venom myotoxic activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
centipede venom myotoxic activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Judith Tabullo De Robles Francisca Fernández Valverde Lucero Valladares Cisneros Juana Hernández Villeda Ayixon Sánchez-Reyes María del Carmen Gutiérrez Mitochondrial activity disruption and local muscle damage induced in mice by Scolopendra polymorpha venom |
topic_facet |
centipede venom myotoxic activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Scolopendra polymorpha (S. polymorpha) is a predatory centipede whose venom contains a multiplicity of biochemical effectors that can cause muscle damage and cumulative cell destruction in its prey. Despite previous investigations of S. polymorpha and other centipede venoms, there is a lack of information on the morphological and biochemical patterns elicited by their myotoxic effects. To elucidate these processes, this paper presents evidence of skeletal muscle damage, and alterations in key biochemical mediators that appear only after exposure to centipede venom. Methods: Venom was collected and fractionated using RP-HPLC; mouse extensor digitorum longus (EDL) muscle was exposed to whole venom and venom fractions to evaluate myotoxicity by means of creatine kinase (CK) - a muscle damage marker - activity measurements and histochemical analysis. Results: CK activity was higher in EDL muscle exposed to venom than in unexposed muscle. This increase was observed after 15 min of venom incubation, and remained stable up to 45 min. Venom-exposed EDL muscle showed signs of muscle damage including necrosis, loss of fascicular structure as well as mitochondrial accumulations and ragged red fibers (RRF), suggesting an impairment in the normal mitochondrial arrangement. Nicotinamide adenine dinucleotide (NADH) and cytochrome oxidase (COX) tests also indicate that respiratory complexes might be affected. Conclusion: Our results suggest a different biochemical composition of S. polymorpha venom, based on the different effects of four venom fractions on the cells tested, according to statistical evidence. Fractions F6 and F7 caused the most important alterations. |
format |
Article in Journal/Newspaper |
author |
Judith Tabullo De Robles Francisca Fernández Valverde Lucero Valladares Cisneros Juana Hernández Villeda Ayixon Sánchez-Reyes María del Carmen Gutiérrez |
author_facet |
Judith Tabullo De Robles Francisca Fernández Valverde Lucero Valladares Cisneros Juana Hernández Villeda Ayixon Sánchez-Reyes María del Carmen Gutiérrez |
author_sort |
Judith Tabullo De Robles |
title |
Mitochondrial activity disruption and local muscle damage induced in mice by Scolopendra polymorpha venom |
title_short |
Mitochondrial activity disruption and local muscle damage induced in mice by Scolopendra polymorpha venom |
title_full |
Mitochondrial activity disruption and local muscle damage induced in mice by Scolopendra polymorpha venom |
title_fullStr |
Mitochondrial activity disruption and local muscle damage induced in mice by Scolopendra polymorpha venom |
title_full_unstemmed |
Mitochondrial activity disruption and local muscle damage induced in mice by Scolopendra polymorpha venom |
title_sort |
mitochondrial activity disruption and local muscle damage induced in mice by scolopendra polymorpha venom |
publisher |
SciELO |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0079 https://doaj.org/article/6d27f7fdfb094f1e96a76de1410cd3af |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100316&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0079 https://doaj.org/article/6d27f7fdfb094f1e96a76de1410cd3af |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0079 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
26 |
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