Multiplex real-time PCR diagnostic of relapsing fevers in Africa.
BACKGROUND:In Africa, relapsing fever borreliae are neglected arthropod-borne pathogens causing mild to deadly septicemia and miscarriage. The closely related Borrelia crocidurae, Borrelia duttonii, Borrelia recurrentis and Borrelia hispanica are rarely diagnosed at the species level, hampering refi...
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ftdoajarticles:oai:doaj.org/article:6ce132bdbeda4633bf5fbcebf6de85b3 2023-05-15T15:08:59+02:00 Multiplex real-time PCR diagnostic of relapsing fevers in Africa. Haitham Elbir Mireille Henry Georges Diatta Oleg Mediannikov Cheikh Sokhna Adama Tall Cristina Socolovschi Sally J Cutler Kassahum D Bilcha Jemal Ali Dayana Campelo Steven C Barker Didier Raoult Michel Drancourt 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002042 https://doaj.org/article/6ce132bdbeda4633bf5fbcebf6de85b3 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3561136?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002042 https://doaj.org/article/6ce132bdbeda4633bf5fbcebf6de85b3 PLoS Neglected Tropical Diseases, Vol 7, Iss 1, p e2042 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002042 2022-12-31T12:23:31Z BACKGROUND:In Africa, relapsing fever borreliae are neglected arthropod-borne pathogens causing mild to deadly septicemia and miscarriage. The closely related Borrelia crocidurae, Borrelia duttonii, Borrelia recurrentis and Borrelia hispanica are rarely diagnosed at the species level, hampering refined epidemiological and clinical knowledge of the relapsing fevers. It would be hugely beneficial to have simultaneous detection and identification of Borrelia to species level directly from clinical samples. METHODOLOGY/PRINCIPAL FINDINGS:We designed a multiplex real-time PCR protocol targeting the 16S rRNA gene detecting all four Borrelia, the glpQ gene specifically detecting B. crocidurae, the recN gene specifically detecting B. duttonii/B. recurrentis and the recC gene specifically detecting B. hispanica. Compared to combined 16S rRNA gene and flaB gene sequencing as the gold standard, multiplex real-time PCR analyses of 171 Borrelia-positive and 101 Borrelia-negative control blood specimens yielded 100% sensitivity and specificity for B. duttonii/B. recurrentis and B. hispanica and 99% sensitivity and specificity for B. crocidurae. CONCLUSIONS/SIGNIFICANCE:The multiplex real-time PCR developed in this study is a rapid technique for both molecular detection and speciation of relapsing fever borreliae from blood in Africa. It could be incorporated in point-of-care laboratory to confirm diagnosis and provide evidence of the burden of infection attributed to different species of known or potentially novel relapsing fever borreliae. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 1 e2042 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Haitham Elbir Mireille Henry Georges Diatta Oleg Mediannikov Cheikh Sokhna Adama Tall Cristina Socolovschi Sally J Cutler Kassahum D Bilcha Jemal Ali Dayana Campelo Steven C Barker Didier Raoult Michel Drancourt Multiplex real-time PCR diagnostic of relapsing fevers in Africa. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:In Africa, relapsing fever borreliae are neglected arthropod-borne pathogens causing mild to deadly septicemia and miscarriage. The closely related Borrelia crocidurae, Borrelia duttonii, Borrelia recurrentis and Borrelia hispanica are rarely diagnosed at the species level, hampering refined epidemiological and clinical knowledge of the relapsing fevers. It would be hugely beneficial to have simultaneous detection and identification of Borrelia to species level directly from clinical samples. METHODOLOGY/PRINCIPAL FINDINGS:We designed a multiplex real-time PCR protocol targeting the 16S rRNA gene detecting all four Borrelia, the glpQ gene specifically detecting B. crocidurae, the recN gene specifically detecting B. duttonii/B. recurrentis and the recC gene specifically detecting B. hispanica. Compared to combined 16S rRNA gene and flaB gene sequencing as the gold standard, multiplex real-time PCR analyses of 171 Borrelia-positive and 101 Borrelia-negative control blood specimens yielded 100% sensitivity and specificity for B. duttonii/B. recurrentis and B. hispanica and 99% sensitivity and specificity for B. crocidurae. CONCLUSIONS/SIGNIFICANCE:The multiplex real-time PCR developed in this study is a rapid technique for both molecular detection and speciation of relapsing fever borreliae from blood in Africa. It could be incorporated in point-of-care laboratory to confirm diagnosis and provide evidence of the burden of infection attributed to different species of known or potentially novel relapsing fever borreliae. |
format |
Article in Journal/Newspaper |
author |
Haitham Elbir Mireille Henry Georges Diatta Oleg Mediannikov Cheikh Sokhna Adama Tall Cristina Socolovschi Sally J Cutler Kassahum D Bilcha Jemal Ali Dayana Campelo Steven C Barker Didier Raoult Michel Drancourt |
author_facet |
Haitham Elbir Mireille Henry Georges Diatta Oleg Mediannikov Cheikh Sokhna Adama Tall Cristina Socolovschi Sally J Cutler Kassahum D Bilcha Jemal Ali Dayana Campelo Steven C Barker Didier Raoult Michel Drancourt |
author_sort |
Haitham Elbir |
title |
Multiplex real-time PCR diagnostic of relapsing fevers in Africa. |
title_short |
Multiplex real-time PCR diagnostic of relapsing fevers in Africa. |
title_full |
Multiplex real-time PCR diagnostic of relapsing fevers in Africa. |
title_fullStr |
Multiplex real-time PCR diagnostic of relapsing fevers in Africa. |
title_full_unstemmed |
Multiplex real-time PCR diagnostic of relapsing fevers in Africa. |
title_sort |
multiplex real-time pcr diagnostic of relapsing fevers in africa. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002042 https://doaj.org/article/6ce132bdbeda4633bf5fbcebf6de85b3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 1, p e2042 (2013) |
op_relation |
http://europepmc.org/articles/PMC3561136?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002042 https://doaj.org/article/6ce132bdbeda4633bf5fbcebf6de85b3 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002042 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
1 |
container_start_page |
e2042 |
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1766340244819410944 |