Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species.
The genus Deschampsia P. Beauv (Poaceae) involves a group of widespread polymorphic species. Some of them are highly tolerant to stressful and variable environmental conditions, and D. antarctica is one of the only two vascular plants growing in Antarctic. This species is a source of useful for sele...
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ftdoajarticles:oai:doaj.org/article:6cb71da6423f47b48f7a2db6eede683b 2023-05-15T14:01:23+02:00 Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species. Alexandra V Amosova Nadezhda L Bolsheva Svyatoslav A Zoshchuk Maryana O Twardovska Olga Yu Yurkevich Igor O Andreev Tatiana E Samatadze Ekaterina D Badaeva Viktor A Kunakh Olga V Muravenko 2017-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0175760 https://doaj.org/article/6cb71da6423f47b48f7a2db6eede683b EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5391082?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0175760 https://doaj.org/article/6cb71da6423f47b48f7a2db6eede683b PLoS ONE, Vol 12, Iss 4, p e0175760 (2017) Medicine R Science Q article 2017 ftdoajarticles https://doi.org/10.1371/journal.pone.0175760 2022-12-31T13:27:47Z The genus Deschampsia P. Beauv (Poaceae) involves a group of widespread polymorphic species. Some of them are highly tolerant to stressful and variable environmental conditions, and D. antarctica is one of the only two vascular plants growing in Antarctic. This species is a source of useful for selection traits and a valuable model for studying an environmental stress tolerance in plants. Genome diversity and comparative chromosomal phylogeny within the genus have not been studied yet as karyotypes of most Deschampsia species are poorly investigated. We firstly conducted a comparative molecular cytogenetic analysis of D. antarctica (Antarctic Peninsula) and related species from various localities (D. cespitosa, D. danthonioides, D. elongata, D. flexuosa (= Avenella flexuosa), D. parvula and D. sukatschewii by fluorescence in situ hybridization with 45S and 5S rDNA, DAPI-banding and sequential rapid in situ hybridization with genomic DNA of D. antarctica, D. cespitosa, and D. flexuosa. Based on patterns of distribution of the examined markers, chromosomes of the studied species were identified. Within these species, common features as well as species peculiarities in their karyotypic structure and chromosomal distribution of molecular cytogenetic markers were characterized. Different chromosomal rearrangements were detected in D. antarctica, D. flexuosa, D. elongata and D. sukatschewii. In karyotypes of D. antarctica, D. cespitosa, D. elongata and D. sukatschewii, 0-3 B chromosomes possessed distinct DAPI-bands were observed. Our findings suggest that the genome evolution of the genus Deschampsia involved polyploidy and also different chromosomal rearrangements. The obtained results will help clarify the relationships within the genus Deschampsia, and can be a basis for the further genetic and biotechnological studies as well as for selection of plants tolerant to extreme habitats. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Antarctic Peninsula PLOS ONE 12 4 e0175760 |
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topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Alexandra V Amosova Nadezhda L Bolsheva Svyatoslav A Zoshchuk Maryana O Twardovska Olga Yu Yurkevich Igor O Andreev Tatiana E Samatadze Ekaterina D Badaeva Viktor A Kunakh Olga V Muravenko Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species. |
topic_facet |
Medicine R Science Q |
description |
The genus Deschampsia P. Beauv (Poaceae) involves a group of widespread polymorphic species. Some of them are highly tolerant to stressful and variable environmental conditions, and D. antarctica is one of the only two vascular plants growing in Antarctic. This species is a source of useful for selection traits and a valuable model for studying an environmental stress tolerance in plants. Genome diversity and comparative chromosomal phylogeny within the genus have not been studied yet as karyotypes of most Deschampsia species are poorly investigated. We firstly conducted a comparative molecular cytogenetic analysis of D. antarctica (Antarctic Peninsula) and related species from various localities (D. cespitosa, D. danthonioides, D. elongata, D. flexuosa (= Avenella flexuosa), D. parvula and D. sukatschewii by fluorescence in situ hybridization with 45S and 5S rDNA, DAPI-banding and sequential rapid in situ hybridization with genomic DNA of D. antarctica, D. cespitosa, and D. flexuosa. Based on patterns of distribution of the examined markers, chromosomes of the studied species were identified. Within these species, common features as well as species peculiarities in their karyotypic structure and chromosomal distribution of molecular cytogenetic markers were characterized. Different chromosomal rearrangements were detected in D. antarctica, D. flexuosa, D. elongata and D. sukatschewii. In karyotypes of D. antarctica, D. cespitosa, D. elongata and D. sukatschewii, 0-3 B chromosomes possessed distinct DAPI-bands were observed. Our findings suggest that the genome evolution of the genus Deschampsia involved polyploidy and also different chromosomal rearrangements. The obtained results will help clarify the relationships within the genus Deschampsia, and can be a basis for the further genetic and biotechnological studies as well as for selection of plants tolerant to extreme habitats. |
format |
Article in Journal/Newspaper |
author |
Alexandra V Amosova Nadezhda L Bolsheva Svyatoslav A Zoshchuk Maryana O Twardovska Olga Yu Yurkevich Igor O Andreev Tatiana E Samatadze Ekaterina D Badaeva Viktor A Kunakh Olga V Muravenko |
author_facet |
Alexandra V Amosova Nadezhda L Bolsheva Svyatoslav A Zoshchuk Maryana O Twardovska Olga Yu Yurkevich Igor O Andreev Tatiana E Samatadze Ekaterina D Badaeva Viktor A Kunakh Olga V Muravenko |
author_sort |
Alexandra V Amosova |
title |
Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species. |
title_short |
Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species. |
title_full |
Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species. |
title_fullStr |
Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species. |
title_full_unstemmed |
Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species. |
title_sort |
comparative molecular cytogenetic characterization of seven deschampsia (poaceae) species. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pone.0175760 https://doaj.org/article/6cb71da6423f47b48f7a2db6eede683b |
geographic |
Antarctic Antarctic Peninsula |
geographic_facet |
Antarctic Antarctic Peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica |
op_source |
PLoS ONE, Vol 12, Iss 4, p e0175760 (2017) |
op_relation |
http://europepmc.org/articles/PMC5391082?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0175760 https://doaj.org/article/6cb71da6423f47b48f7a2db6eede683b |
op_doi |
https://doi.org/10.1371/journal.pone.0175760 |
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PLOS ONE |
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12 |
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4 |
container_start_page |
e0175760 |
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1766271191326130176 |