Evaluación de la baciloscopia, cultivo y reacción en cadena de la polimerasa para el diagnóstico de tuberculosis pulmonar

• Main Authors: Nava Paz Orlando, Hassanhi Manzur, Prieto Lisbeth
• Format: Article in Journal/Newspaper
• Language: English; Spanish
• Published: Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales 2005
• Subjects: Tuberculosis; RCP; direct exam; cultivation; Examen directo; cultivo; Arctic medicine. Tropical medicine; RC955-962; Public aspects of medicine; RA1-1270; Arctic; Cadena
• Online Access: https://doaj.org/article/6cb31e55e9d64380bda2ff3dad090a48

Abstract: The utility of baciloscopy, cultivation and Polymerase Chain Reaction of (PCR) was evaluated in 31 sputum samples, of patient of both sexes, without distinction of etnial group and ages between 17 and 80 years, with clinical manifestations, discoveries radiological and/or suggestive epidemic data of lung tuberculosis. To all the samples they were practiced baciloscopy using the technique of Ziehl-Neelsen. The Ogawa-Kudoh cultivation was used like standard. The objectives for the rehearsal of PCR were the genes that code the protein 32-KDa (alpha antigen) and the insertion sequence IS6110. After the amplification was obtained a fragment of 984 couples of bases corresponding to the sequence of insert IS6110 and another of 506 couples corresponding to the alpha antigen. Of the total of samples examined by the Ziehl-Neelsen method, 19 were positive and 12 were negative (61.29 and 38.71% respectively). 19 samples (61.29%) were positive and 12 (38.71%) were negative to the cultivation. Of all amplified samples, 22 (70.69%) showed the presence of fragments corresponding to DNA so much to the sequence of insert IS6110 like the antigen alpha 32-KDa, while in 9 (20.04%) presence of DNA mycobacterium was not detected for RCP method. The sensibility of the RCP test was virtually 100% and its specificity was of 100%. It was demonstrated that RCP, based on the simultaneous amplification of the insertion element IS6110 and the gene that codes the alpha antigen in a single step, can identify mycobacterium infections. We conclude also that is more sensitive that routine bacteriological procedures (direct exam and cultivation) and can be a very powerful tool for the diagnosis of lung tuberculosis and other mycobacterium diseases. Resumen La utilidad de la baciloscopia, cultivo y la Reacción en Cadena de la Polimerasa (RCP) fue evaluada en 31 muestras de esputo, provenientes de pacientes de ambos sexos, sin distinción de grupo étnico y edades comprendidas entre los 17 y 80 años, con manifestaciones clínicas, hallazgos ...