Identification of regenerating island-derived protein 3E in dogs
Regenerating islet-derived protein (REG) 1A (aka pancreatic stone protein) and REG3A (aka pancreatitis-associated protein) are upregulated in humans with sepsis, pancreatitis, and gastrointestinal diseases, but little is known about this protein family in dogs. Our aim was to identify REG1 and REG3...
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ftdoajarticles:oai:doaj.org/article:6c76c7a4e51045f790b57ef980ba871c 2023-05-15T15:50:49+02:00 Identification of regenerating island-derived protein 3E in dogs Laureen M. Peters Judith Howard Tosso Leeb Meike Mevissen Rolf Graf Theresia Reding Graf 2022-10-01T00:00:00Z https://doi.org/10.3389/fvets.2022.1010809 https://doaj.org/article/6c76c7a4e51045f790b57ef980ba871c EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fvets.2022.1010809/full https://doaj.org/toc/2297-1769 2297-1769 doi:10.3389/fvets.2022.1010809 https://doaj.org/article/6c76c7a4e51045f790b57ef980ba871c Frontiers in Veterinary Science, Vol 9 (2022) biomarker canine Canis lupus familiaris gastrointestinal pancreas pancreatitis Veterinary medicine SF600-1100 article 2022 ftdoajarticles https://doi.org/10.3389/fvets.2022.1010809 2022-12-30T23:06:05Z Regenerating islet-derived protein (REG) 1A (aka pancreatic stone protein) and REG3A (aka pancreatitis-associated protein) are upregulated in humans with sepsis, pancreatitis, and gastrointestinal diseases, but little is known about this protein family in dogs. Our aim was to identify REG1 and REG3 family members in dogs. REG-family genes were computationally annotated in the canine genome and proteome, with verification of gene expression using publicly available RNA-seq data. The presence of the protein in canine pancreatic tissue and plasma was investigated with Western blot and immunohistochemistry, using anti-human REG1A and REG3A antibodies. Protein identity was confirmed with mass spectrometry. Two members of the REG3 subfamily were found in the canine genome, REG3E1 and REG3E2, both encoding for the same 176 AA protein, subsequently named REG3E. Anti-human REG3A antibodies demonstrated cross-reactivity with the canine REG3E protein in pancreas homogenates. In canine plasma, a protein band of approximately 17 kDa was apparent. Mass spectrometry confirmed this protein to be the product of the two annotated REG3E genes. Strong immunoreactivity to anti-human REG3A antibodies was found in sections of canine pancreas affected with acute pancreatitis, but it was weak in healthy pancreatic tissue. Recombinant canine REG3E protein underwent a selective trypsin digestion as described in other species. No evidence for the presence of a homolog of REG1A in dogs was found in any of the investigations. In conclusion, dogs express REG3E in the pancreas, whose role as biomarker merits further investigations. Homologs to human REG1A are not likely to exist in dogs. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Frontiers in Veterinary Science 9 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
biomarker canine Canis lupus familiaris gastrointestinal pancreas pancreatitis Veterinary medicine SF600-1100 |
spellingShingle |
biomarker canine Canis lupus familiaris gastrointestinal pancreas pancreatitis Veterinary medicine SF600-1100 Laureen M. Peters Judith Howard Tosso Leeb Meike Mevissen Rolf Graf Theresia Reding Graf Identification of regenerating island-derived protein 3E in dogs |
topic_facet |
biomarker canine Canis lupus familiaris gastrointestinal pancreas pancreatitis Veterinary medicine SF600-1100 |
description |
Regenerating islet-derived protein (REG) 1A (aka pancreatic stone protein) and REG3A (aka pancreatitis-associated protein) are upregulated in humans with sepsis, pancreatitis, and gastrointestinal diseases, but little is known about this protein family in dogs. Our aim was to identify REG1 and REG3 family members in dogs. REG-family genes were computationally annotated in the canine genome and proteome, with verification of gene expression using publicly available RNA-seq data. The presence of the protein in canine pancreatic tissue and plasma was investigated with Western blot and immunohistochemistry, using anti-human REG1A and REG3A antibodies. Protein identity was confirmed with mass spectrometry. Two members of the REG3 subfamily were found in the canine genome, REG3E1 and REG3E2, both encoding for the same 176 AA protein, subsequently named REG3E. Anti-human REG3A antibodies demonstrated cross-reactivity with the canine REG3E protein in pancreas homogenates. In canine plasma, a protein band of approximately 17 kDa was apparent. Mass spectrometry confirmed this protein to be the product of the two annotated REG3E genes. Strong immunoreactivity to anti-human REG3A antibodies was found in sections of canine pancreas affected with acute pancreatitis, but it was weak in healthy pancreatic tissue. Recombinant canine REG3E protein underwent a selective trypsin digestion as described in other species. No evidence for the presence of a homolog of REG1A in dogs was found in any of the investigations. In conclusion, dogs express REG3E in the pancreas, whose role as biomarker merits further investigations. Homologs to human REG1A are not likely to exist in dogs. |
format |
Article in Journal/Newspaper |
author |
Laureen M. Peters Judith Howard Tosso Leeb Meike Mevissen Rolf Graf Theresia Reding Graf |
author_facet |
Laureen M. Peters Judith Howard Tosso Leeb Meike Mevissen Rolf Graf Theresia Reding Graf |
author_sort |
Laureen M. Peters |
title |
Identification of regenerating island-derived protein 3E in dogs |
title_short |
Identification of regenerating island-derived protein 3E in dogs |
title_full |
Identification of regenerating island-derived protein 3E in dogs |
title_fullStr |
Identification of regenerating island-derived protein 3E in dogs |
title_full_unstemmed |
Identification of regenerating island-derived protein 3E in dogs |
title_sort |
identification of regenerating island-derived protein 3e in dogs |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fvets.2022.1010809 https://doaj.org/article/6c76c7a4e51045f790b57ef980ba871c |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Frontiers in Veterinary Science, Vol 9 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fvets.2022.1010809/full https://doaj.org/toc/2297-1769 2297-1769 doi:10.3389/fvets.2022.1010809 https://doaj.org/article/6c76c7a4e51045f790b57ef980ba871c |
op_doi |
https://doi.org/10.3389/fvets.2022.1010809 |
container_title |
Frontiers in Veterinary Science |
container_volume |
9 |
_version_ |
1766385835102437376 |