Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites
Abstract Background Malaria transmission occurs during the blood feeding of infected anopheline mosquitoes concomitant with a saliva injection into the vertebrate host. In sub-Saharan Africa, most malaria transmission is due to Anopheles funestus s.s and to Anopheles gambiae s.l. (mainly Anopheles g...
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ftdoajarticles:oai:doaj.org/article:6c5e33268ffa44caae358f8320d7c778 2023-05-15T15:14:18+02:00 Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites Ali Zakia M I Bakli Mahfoud Fontaine Albin Bakkali Nawal Hai Vinh Vu Audebert Stephane Boublik Yvan Pagès Frederic Remoué Franck Rogier Christophe Fraisier Christophe Almeras Lionel 2012-12-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-439 https://doaj.org/article/6c5e33268ffa44caae358f8320d7c778 EN eng BMC http://www.malariajournal.com/content/11/1/439 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-439 1475-2875 https://doaj.org/article/6c5e33268ffa44caae358f8320d7c778 Malaria Journal, Vol 11, Iss 1, p 439 (2012) Anopheles gambiae Anopheles funestus Antigenic salivary proteins SG6 5′nucleotidase Exposure biomarkers Epidemiology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-439 2022-12-31T13:50:13Z Abstract Background Malaria transmission occurs during the blood feeding of infected anopheline mosquitoes concomitant with a saliva injection into the vertebrate host. In sub-Saharan Africa, most malaria transmission is due to Anopheles funestus s.s and to Anopheles gambiae s.l. (mainly Anopheles gambiae s.s. and Anopheles arabiensis ). Several studies have demonstrated that the immune response against salivary antigens could be used to evaluate individual exposure to mosquito bites. The aim of this study was to assess the use of secreted salivary proteins as specific biomarkers of exposure to An . gambiae and/or An . funestus bites. Methods For this purpose, salivary gland proteins 6 (SG6) and 5′nucleotidases (5′nuc) from An. gambiae (gSG6 and g-5′nuc) and An. funestus (fSG6 and f-5′nuc) were selected and produced in recombinant form. The specificity of the IgG response against these salivary proteins was tested using an ELISA with sera from individuals living in three Senegalese villages (NDiop, n = 50; Dielmo, n = 38; and Diama, n = 46) that had been exposed to distinct densities and proportions of the Anopheles species. Individuals who had not been exposed to these tropical mosquitoes were used as controls (Marseille, n = 45). Results The IgG responses against SG6 recombinant proteins from these two Anopheles species and against g-5′nucleotidase from An . gambiae , were significantly higher in Senegalese individuals compared with controls who were not exposed to specific Anopheles species. Conversely, an association was observed between the level of An. funestus exposure and the serological immune response levels against the f-5′nucleotidase protein . Conclusion This study revealed an Anopheles salivary antigenic protein that could be considered to be a promising antigenic marker to distinguish malaria vector exposure at the species level. The epidemiological interest of such species-specific antigenic markers is discussed. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 439 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Anopheles gambiae Anopheles funestus Antigenic salivary proteins SG6 5′nucleotidase Exposure biomarkers Epidemiology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Anopheles gambiae Anopheles funestus Antigenic salivary proteins SG6 5′nucleotidase Exposure biomarkers Epidemiology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Ali Zakia M I Bakli Mahfoud Fontaine Albin Bakkali Nawal Hai Vinh Vu Audebert Stephane Boublik Yvan Pagès Frederic Remoué Franck Rogier Christophe Fraisier Christophe Almeras Lionel Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites |
topic_facet |
Anopheles gambiae Anopheles funestus Antigenic salivary proteins SG6 5′nucleotidase Exposure biomarkers Epidemiology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria transmission occurs during the blood feeding of infected anopheline mosquitoes concomitant with a saliva injection into the vertebrate host. In sub-Saharan Africa, most malaria transmission is due to Anopheles funestus s.s and to Anopheles gambiae s.l. (mainly Anopheles gambiae s.s. and Anopheles arabiensis ). Several studies have demonstrated that the immune response against salivary antigens could be used to evaluate individual exposure to mosquito bites. The aim of this study was to assess the use of secreted salivary proteins as specific biomarkers of exposure to An . gambiae and/or An . funestus bites. Methods For this purpose, salivary gland proteins 6 (SG6) and 5′nucleotidases (5′nuc) from An. gambiae (gSG6 and g-5′nuc) and An. funestus (fSG6 and f-5′nuc) were selected and produced in recombinant form. The specificity of the IgG response against these salivary proteins was tested using an ELISA with sera from individuals living in three Senegalese villages (NDiop, n = 50; Dielmo, n = 38; and Diama, n = 46) that had been exposed to distinct densities and proportions of the Anopheles species. Individuals who had not been exposed to these tropical mosquitoes were used as controls (Marseille, n = 45). Results The IgG responses against SG6 recombinant proteins from these two Anopheles species and against g-5′nucleotidase from An . gambiae , were significantly higher in Senegalese individuals compared with controls who were not exposed to specific Anopheles species. Conversely, an association was observed between the level of An. funestus exposure and the serological immune response levels against the f-5′nucleotidase protein . Conclusion This study revealed an Anopheles salivary antigenic protein that could be considered to be a promising antigenic marker to distinguish malaria vector exposure at the species level. The epidemiological interest of such species-specific antigenic markers is discussed. |
format |
Article in Journal/Newspaper |
author |
Ali Zakia M I Bakli Mahfoud Fontaine Albin Bakkali Nawal Hai Vinh Vu Audebert Stephane Boublik Yvan Pagès Frederic Remoué Franck Rogier Christophe Fraisier Christophe Almeras Lionel |
author_facet |
Ali Zakia M I Bakli Mahfoud Fontaine Albin Bakkali Nawal Hai Vinh Vu Audebert Stephane Boublik Yvan Pagès Frederic Remoué Franck Rogier Christophe Fraisier Christophe Almeras Lionel |
author_sort |
Ali Zakia M I |
title |
Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites |
title_short |
Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites |
title_full |
Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites |
title_fullStr |
Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites |
title_full_unstemmed |
Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites |
title_sort |
assessment of anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-439 https://doaj.org/article/6c5e33268ffa44caae358f8320d7c778 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 439 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/439 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-439 1475-2875 https://doaj.org/article/6c5e33268ffa44caae358f8320d7c778 |
op_doi |
https://doi.org/10.1186/1475-2875-11-439 |
container_title |
Malaria Journal |
container_volume |
11 |
container_issue |
1 |
container_start_page |
439 |
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1766344767967330304 |