Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation

• Published in: International Journal of Molecular Sciences
• Main Authors: Rodolpho R. C. Monteiro, Davino M. Andrade Neto, Pierre B. A. Fechine, Ada A. S. Lopes, Luciana R. B. Gonçalves, José C. S. dos Santos, Maria C. M. de Souza, Roberto Fernandez-Lafuente
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2019
• Subjects: ethyl butyrate; lipase a from candida antarctica; lipase b from candida antarctica; magnetic nanoparticles; ultrasonic irradiation; Biology (General); QH301-705.5; Chemistry; QD1-999; Antarc*; Antarctica
• Online Access: https://doi.org/10.3390/ijms20225807; https://doaj.org/article/6c33dbc28eb0455b96435cb6ef58b630

The synthesis of ethyl butyrate catalyzed by lipases A (CALA) or B (CALB) from Candida antarctica immobilized onto magnetic nanoparticles (MNP), CALA-MNP and CALB-MNP, respectively, is hereby reported. MNPs were prepared by co-precipitation, functionalized with 3-aminopropyltriethoxysilane, activated with glutaraldehyde, and then used as support to immobilize either CALA or CALB (immobilization yield: 100 ± 1.2% and 57.6 ± 3.8%; biocatalysts activities: 198.3 ± 2.7 U p -NPB /g and 52.9 ± 1.7 U p -NPB /g for CALA-MNP and CALB-MNP, respectively). X-ray diffraction and Raman spectroscopy analysis indicated the production of a magnetic nanomaterial with a diameter of 13.0 nm, whereas Fourier-transform infrared spectroscopy indicated functionalization, activation and enzyme immobilization. To determine the optimum conditions for the synthesis, a four-variable Central Composite Design (CCD) (biocatalyst content, molar ratio, temperature and time) was performed. Under optimized conditions (1:1, 45 °C and 6 h), it was possible to achieve 99.2 ± 0.3% of conversion for CALA-MNP (10 mg) and 97.5 ± 0.8% for CALB-MNP (12.5 mg), which retained approximately 80% of their activity after 10 consecutive cycles of esterification. Under ultrasonic irradiation, similar conversions were achieved but at 4 h of incubation, demonstrating the efficiency of ultrasound technology in the enzymatic synthesis of esters.