Multidrug resistance extended spectrum β-lactamase and AmpC producing Escherichia coli isolated from the environment of Bogor Slaughterhouse, Indonesia

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Mirnawati Bachrum Sudarwanto, Denny Widaya Lukman, Trioso Purnawarman, Hadri Latif, Herwin Pisestyani, Eddy Sukmawinata
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2017
• Subjects: Escherichia coli; Environment; Extended spectrum β-lactamase; Slaughterhouse; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic; Human health
• Online Access: https://doi.org/10.1016/j.apjtb.2017.07.012; https://doaj.org/article/6bdc334ce8a44ab5876b68330766687e

Objective: To determine the multidrug resistance extended spectrum β-lactamase and AmpC (ESBL/AmpC producing) Escherichia coli (E. coli) isolated from the environment of Bogor slaughterhouse, Indonesia. Methods: A total of 35 samples from 7 locations in slaughterhouse i.e., source of water, slaughtering floor, swab of carcass area floor, swab of evisceration area floor, untreated waste water, treated waste water, drinking water for cattle were collected from March to April 2016. Presence of ESBL/AmpC producing E. coli and susceptibility testing against 8 antimicrobial agents (penicillin G, streptomycin, gentamycin, ciprofloxacin, enrofloxacin, tetracycline, trimethoprim-sulfamethoxazole, and polymyxin B) were detected by disk diffusion test according to Clinical and Laboratory Standards Institute. Results: ESBL/AmpC producing E. coli were identified in 14.3% (5/35) of the collected samples from the environment of Bogor slaughterhouse. ESBL/AmpC-producing E. coli isolates were detected in untreated waste water (n = 3), slaughtering floor (n = 1), and carcass area floor (n = 1). Most of ESBL/AmpC-producing E. coli isolates (80%) showed multidrug resistance phenotypes against at least three classes of antibiotics. The highest incidence of antibiotics resistance was against penicillin G (100.0%) and streptomycin (100.0%), followed by gentamicin (60.0%), trimethoprim-sulfamethoxazole (60.0%), tetracycline (40.0%), ciprofloxacin (40.0%), enrofloxacin (20.0%), and polymyxin B (0.0%). Conclusions: The transmission of antimicrobial resistant bacteria into the environment may be a potential risk for human health.