Hot in Cold: Microbial Life in the Hottest Springs in Permafrost
Chukotka is an arctic region located in the continuous permafrost zone, but thermal springs are abundant there. In this study, for the first time, the microbial communities of the Chukotka hot springs (CHS) biofilms and sediments with temperatures 54–94 °C were investigated and analyzed by NGS seque...
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ftdoajarticles:oai:doaj.org/article:6ba1eb1a096240939f046b50ed08c546 2023-05-15T15:07:41+02:00 Hot in Cold: Microbial Life in the Hottest Springs in Permafrost Tatiana V. Kochetkova Stepan V. Toshchakov Kseniya S. Zayulina Alexander G. Elcheninov Daria G. Zavarzina Vasiliy Yu. Lavrushin Elizaveta A. Bonch-Osmolovskaya Ilya V. Kublanov 2020-08-01T00:00:00Z https://doi.org/10.3390/microorganisms8091308 https://doaj.org/article/6ba1eb1a096240939f046b50ed08c546 EN eng MDPI AG https://www.mdpi.com/2076-2607/8/9/1308 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms8091308 2076-2607 https://doaj.org/article/6ba1eb1a096240939f046b50ed08c546 Microorganisms, Vol 8, Iss 1308, p 1308 (2020) Chukotka thermophiles hot spring NGS sequencing microbial diversity permafrost Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.3390/microorganisms8091308 2022-12-31T10:03:46Z Chukotka is an arctic region located in the continuous permafrost zone, but thermal springs are abundant there. In this study, for the first time, the microbial communities of the Chukotka hot springs (CHS) biofilms and sediments with temperatures 54–94 °C were investigated and analyzed by NGS sequencing of 16S rRNA gene amplicons. In microbial mats (54–75 °C), phototrophic bacteria of genus Chloroflexus dominated (up to 89% of all prokaryotes), while Aquificae were the most numerous at higher temperatures in Fe-rich sediments and filamentous “streamers” (up to 92%). The electron donors typical for Aquificae , such as H 2 S and H 2 , are absent or present only in trace amounts, and the prevalence of Aquificae might be connected with their ability to oxidize the ferrous iron present in CHS sediments. Armatimonadetes , Proteobacteria , Deinococcus-Thermus , Dictyoglomi , and Thermotogae, as well as uncultured bacteria (candidate divisions Oct-Spa1-106, GAL15, and OPB56), were numerous, and Cyanobacteria were present in low numbers. Archaea (less than 8% of the total community of each tested spring) belonged to Bathyarchaeota , Aigarchaeota , and Thaumarchaeota . The geographical location and the predominantly autotrophic microbial community, built on mechanisms other than the sulfur cycle-based ones, make CHS a special and unique terrestrial geothermal ecosystem. Article in Journal/Newspaper Arctic Chukotka permafrost Directory of Open Access Journals: DOAJ Articles Arctic Microorganisms 8 9 1308 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Chukotka thermophiles hot spring NGS sequencing microbial diversity permafrost Biology (General) QH301-705.5 |
spellingShingle |
Chukotka thermophiles hot spring NGS sequencing microbial diversity permafrost Biology (General) QH301-705.5 Tatiana V. Kochetkova Stepan V. Toshchakov Kseniya S. Zayulina Alexander G. Elcheninov Daria G. Zavarzina Vasiliy Yu. Lavrushin Elizaveta A. Bonch-Osmolovskaya Ilya V. Kublanov Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
topic_facet |
Chukotka thermophiles hot spring NGS sequencing microbial diversity permafrost Biology (General) QH301-705.5 |
description |
Chukotka is an arctic region located in the continuous permafrost zone, but thermal springs are abundant there. In this study, for the first time, the microbial communities of the Chukotka hot springs (CHS) biofilms and sediments with temperatures 54–94 °C were investigated and analyzed by NGS sequencing of 16S rRNA gene amplicons. In microbial mats (54–75 °C), phototrophic bacteria of genus Chloroflexus dominated (up to 89% of all prokaryotes), while Aquificae were the most numerous at higher temperatures in Fe-rich sediments and filamentous “streamers” (up to 92%). The electron donors typical for Aquificae , such as H 2 S and H 2 , are absent or present only in trace amounts, and the prevalence of Aquificae might be connected with their ability to oxidize the ferrous iron present in CHS sediments. Armatimonadetes , Proteobacteria , Deinococcus-Thermus , Dictyoglomi , and Thermotogae, as well as uncultured bacteria (candidate divisions Oct-Spa1-106, GAL15, and OPB56), were numerous, and Cyanobacteria were present in low numbers. Archaea (less than 8% of the total community of each tested spring) belonged to Bathyarchaeota , Aigarchaeota , and Thaumarchaeota . The geographical location and the predominantly autotrophic microbial community, built on mechanisms other than the sulfur cycle-based ones, make CHS a special and unique terrestrial geothermal ecosystem. |
format |
Article in Journal/Newspaper |
author |
Tatiana V. Kochetkova Stepan V. Toshchakov Kseniya S. Zayulina Alexander G. Elcheninov Daria G. Zavarzina Vasiliy Yu. Lavrushin Elizaveta A. Bonch-Osmolovskaya Ilya V. Kublanov |
author_facet |
Tatiana V. Kochetkova Stepan V. Toshchakov Kseniya S. Zayulina Alexander G. Elcheninov Daria G. Zavarzina Vasiliy Yu. Lavrushin Elizaveta A. Bonch-Osmolovskaya Ilya V. Kublanov |
author_sort |
Tatiana V. Kochetkova |
title |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_short |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_full |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_fullStr |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_full_unstemmed |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_sort |
hot in cold: microbial life in the hottest springs in permafrost |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8091308 https://doaj.org/article/6ba1eb1a096240939f046b50ed08c546 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Chukotka permafrost |
genre_facet |
Arctic Chukotka permafrost |
op_source |
Microorganisms, Vol 8, Iss 1308, p 1308 (2020) |
op_relation |
https://www.mdpi.com/2076-2607/8/9/1308 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms8091308 2076-2607 https://doaj.org/article/6ba1eb1a096240939f046b50ed08c546 |
op_doi |
https://doi.org/10.3390/microorganisms8091308 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
9 |
container_start_page |
1308 |
_version_ |
1766339137443463168 |