Genetic analysis of the frozen microbiome at 7900 m a.s.l., on the South Col of Sagarmatha (Mount Everest)
ABSTRACTMicrobial communities in alpine environments >7,500 m.a.s.l. have not been well studied using modern cultivation-independent sequencing approaches due to the challenges and danger associated with reaching such high elevations. For this reason, we know little about the microorganisms found...
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ftdoajarticles:oai:doaj.org/article:6b97e5304088480b9b0cc159d04d0075 2024-09-15T17:49:02+00:00 Genetic analysis of the frozen microbiome at 7900 m a.s.l., on the South Col of Sagarmatha (Mount Everest) Nicholas B. Dragone L. Baker Perry Adam J. Solon Anton Seimon Tracie A. Seimon Steven K. Schmidt 2023-12-01T00:00:00Z https://doi.org/10.1080/15230430.2023.2164999 https://doaj.org/article/6b97e5304088480b9b0cc159d04d0075 EN eng Taylor & Francis Group https://www.tandfonline.com/doi/10.1080/15230430.2023.2164999 https://doaj.org/toc/1523-0430 https://doaj.org/toc/1938-4246 doi:10.1080/15230430.2023.2164999 1938-4246 1523-0430 https://doaj.org/article/6b97e5304088480b9b0cc159d04d0075 Arctic, Antarctic, and Alpine Research, Vol 55, Iss 1 (2023) Mount Everest alpine microbial ecology microbiology Environmental sciences GE1-350 Ecology QH540-549.5 article 2023 ftdoajarticles https://doi.org/10.1080/15230430.2023.2164999 2024-08-05T17:49:40Z ABSTRACTMicrobial communities in alpine environments >7,500 m.a.s.l. have not been well studied using modern cultivation-independent sequencing approaches due to the challenges and danger associated with reaching such high elevations. For this reason, we know little about the microorganisms found in sediments on Earth’s tallest mountains, how they reach these surfaces, and how they survive and remain active at such extreme elevations. Here, we explore the microbial diversity recovered from three sediment samples collected from the South Col (~7,900 m.a.s.l.) of Sagarmatha (Mount Everest) using both culturing and next generation sequencing approaches (16S rRNA gene, internal transcribed spacer [ITS] region, and 18S rRNA gene sequencing). Both approaches detected very low diversity of bacteria, protists, and fungi that included a combination of cosmopolitan taxa and specialized microorganisms often found at high elevations like those of the genera Modestobacter and Naganishia. Though we managed to grow viable cultures of many of these taxa, it remains likely that few, if any, can be active in situ at the South Col. Instead, these high-elevation surfaces may act as deep-freeze collection zones of organisms deposited from the atmosphere or left by climbers scaling the Earth’s highest mountain. Article in Journal/Newspaper Antarctic and Alpine Research Arctic Directory of Open Access Journals: DOAJ Articles Arctic, Antarctic, and Alpine Research 55 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Mount Everest alpine microbial ecology microbiology Environmental sciences GE1-350 Ecology QH540-549.5 |
spellingShingle |
Mount Everest alpine microbial ecology microbiology Environmental sciences GE1-350 Ecology QH540-549.5 Nicholas B. Dragone L. Baker Perry Adam J. Solon Anton Seimon Tracie A. Seimon Steven K. Schmidt Genetic analysis of the frozen microbiome at 7900 m a.s.l., on the South Col of Sagarmatha (Mount Everest) |
topic_facet |
Mount Everest alpine microbial ecology microbiology Environmental sciences GE1-350 Ecology QH540-549.5 |
description |
ABSTRACTMicrobial communities in alpine environments >7,500 m.a.s.l. have not been well studied using modern cultivation-independent sequencing approaches due to the challenges and danger associated with reaching such high elevations. For this reason, we know little about the microorganisms found in sediments on Earth’s tallest mountains, how they reach these surfaces, and how they survive and remain active at such extreme elevations. Here, we explore the microbial diversity recovered from three sediment samples collected from the South Col (~7,900 m.a.s.l.) of Sagarmatha (Mount Everest) using both culturing and next generation sequencing approaches (16S rRNA gene, internal transcribed spacer [ITS] region, and 18S rRNA gene sequencing). Both approaches detected very low diversity of bacteria, protists, and fungi that included a combination of cosmopolitan taxa and specialized microorganisms often found at high elevations like those of the genera Modestobacter and Naganishia. Though we managed to grow viable cultures of many of these taxa, it remains likely that few, if any, can be active in situ at the South Col. Instead, these high-elevation surfaces may act as deep-freeze collection zones of organisms deposited from the atmosphere or left by climbers scaling the Earth’s highest mountain. |
format |
Article in Journal/Newspaper |
author |
Nicholas B. Dragone L. Baker Perry Adam J. Solon Anton Seimon Tracie A. Seimon Steven K. Schmidt |
author_facet |
Nicholas B. Dragone L. Baker Perry Adam J. Solon Anton Seimon Tracie A. Seimon Steven K. Schmidt |
author_sort |
Nicholas B. Dragone |
title |
Genetic analysis of the frozen microbiome at 7900 m a.s.l., on the South Col of Sagarmatha (Mount Everest) |
title_short |
Genetic analysis of the frozen microbiome at 7900 m a.s.l., on the South Col of Sagarmatha (Mount Everest) |
title_full |
Genetic analysis of the frozen microbiome at 7900 m a.s.l., on the South Col of Sagarmatha (Mount Everest) |
title_fullStr |
Genetic analysis of the frozen microbiome at 7900 m a.s.l., on the South Col of Sagarmatha (Mount Everest) |
title_full_unstemmed |
Genetic analysis of the frozen microbiome at 7900 m a.s.l., on the South Col of Sagarmatha (Mount Everest) |
title_sort |
genetic analysis of the frozen microbiome at 7900 m a.s.l., on the south col of sagarmatha (mount everest) |
publisher |
Taylor & Francis Group |
publishDate |
2023 |
url |
https://doi.org/10.1080/15230430.2023.2164999 https://doaj.org/article/6b97e5304088480b9b0cc159d04d0075 |
genre |
Antarctic and Alpine Research Arctic |
genre_facet |
Antarctic and Alpine Research Arctic |
op_source |
Arctic, Antarctic, and Alpine Research, Vol 55, Iss 1 (2023) |
op_relation |
https://www.tandfonline.com/doi/10.1080/15230430.2023.2164999 https://doaj.org/toc/1523-0430 https://doaj.org/toc/1938-4246 doi:10.1080/15230430.2023.2164999 1938-4246 1523-0430 https://doaj.org/article/6b97e5304088480b9b0cc159d04d0075 |
op_doi |
https://doi.org/10.1080/15230430.2023.2164999 |
container_title |
Arctic, Antarctic, and Alpine Research |
container_volume |
55 |
container_issue |
1 |
_version_ |
1810290742569992192 |