Genetic and phenotypic characterization of recently discovered enterovirus D type 111.
Members of the species Enterovirus D (EV-D) remain poorly studied. The two first EV-D types (EV-D68 and EV-D70) have regularly caused outbreaks in humans since their discovery five decades ago but have been neglected until the recent occurrence of severe respiratory diseases due to EV-D68. The three...
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ftdoajarticles:oai:doaj.org/article:6b4569f9f3fb4b7ca6698445b1bf7595 2023-05-15T15:15:29+02:00 Genetic and phenotypic characterization of recently discovered enterovirus D type 111. Serge Alain Sadeuh-Mba Marie-Line Joffret Arthur Mazitchi Marie-Claire Endegue-Zanga Richard Njouom Francis Delpeyroux Ionela Gouandjika-Vasilache Maël Bessaud 2019-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007797 https://doaj.org/article/6b4569f9f3fb4b7ca6698445b1bf7595 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007797 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007797 https://doaj.org/article/6b4569f9f3fb4b7ca6698445b1bf7595 PLoS Neglected Tropical Diseases, Vol 13, Iss 10, p e0007797 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007797 2022-12-31T05:52:39Z Members of the species Enterovirus D (EV-D) remain poorly studied. The two first EV-D types (EV-D68 and EV-D70) have regularly caused outbreaks in humans since their discovery five decades ago but have been neglected until the recent occurrence of severe respiratory diseases due to EV-D68. The three other known EV-D types (EV-D94, EV-D111 and EV-D120) were discovered in the 2000s-2010s in Africa and have never been observed elsewhere. One strain of EV-D111 and all known EV-D120s were detected in stool samples of wild non-human primates, suggesting that these viruses could be zoonotic viruses. To date, EV-D111s are only known through partial genetic sequences of the few strains that have been identified so far. In an attempt to bring new pieces to the puzzle, we genetically characterized four EV-D111 strains (among the seven that have been reported until now). We observed that the EV-D111 strains from human samples and the unique simian EV-D111 strain were not phylogenetically distinct, thus suggesting a recent zoonotic transmission. We also discovered evidences of probable intertypic genetic recombination events between EV-D111s and EV-D94s. As recombination can only happen in co-infected cells, this suggests that EV-D94s and EV-D111s share common replication sites in the infected hosts. These sites could be located in the gut since the phenotypic analysis we performed showed that, contrary to EV-D68s and like EV-D94s, EV-D111s are resistant to acid pHs. We also found that EV-D111s induce strong cytopathic effects on L20B cells, a cell line routinely used to specifically detect polioviruses. An active circulation of EV-D111s among humans could then induce a high number of false-positive detection of polioviruses, which could be particularly problematic in Central Africa, where EV-D111 circulates and which is a key region for poliovirus eradication. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 10 e0007797 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Serge Alain Sadeuh-Mba Marie-Line Joffret Arthur Mazitchi Marie-Claire Endegue-Zanga Richard Njouom Francis Delpeyroux Ionela Gouandjika-Vasilache Maël Bessaud Genetic and phenotypic characterization of recently discovered enterovirus D type 111. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Members of the species Enterovirus D (EV-D) remain poorly studied. The two first EV-D types (EV-D68 and EV-D70) have regularly caused outbreaks in humans since their discovery five decades ago but have been neglected until the recent occurrence of severe respiratory diseases due to EV-D68. The three other known EV-D types (EV-D94, EV-D111 and EV-D120) were discovered in the 2000s-2010s in Africa and have never been observed elsewhere. One strain of EV-D111 and all known EV-D120s were detected in stool samples of wild non-human primates, suggesting that these viruses could be zoonotic viruses. To date, EV-D111s are only known through partial genetic sequences of the few strains that have been identified so far. In an attempt to bring new pieces to the puzzle, we genetically characterized four EV-D111 strains (among the seven that have been reported until now). We observed that the EV-D111 strains from human samples and the unique simian EV-D111 strain were not phylogenetically distinct, thus suggesting a recent zoonotic transmission. We also discovered evidences of probable intertypic genetic recombination events between EV-D111s and EV-D94s. As recombination can only happen in co-infected cells, this suggests that EV-D94s and EV-D111s share common replication sites in the infected hosts. These sites could be located in the gut since the phenotypic analysis we performed showed that, contrary to EV-D68s and like EV-D94s, EV-D111s are resistant to acid pHs. We also found that EV-D111s induce strong cytopathic effects on L20B cells, a cell line routinely used to specifically detect polioviruses. An active circulation of EV-D111s among humans could then induce a high number of false-positive detection of polioviruses, which could be particularly problematic in Central Africa, where EV-D111 circulates and which is a key region for poliovirus eradication. |
format |
Article in Journal/Newspaper |
author |
Serge Alain Sadeuh-Mba Marie-Line Joffret Arthur Mazitchi Marie-Claire Endegue-Zanga Richard Njouom Francis Delpeyroux Ionela Gouandjika-Vasilache Maël Bessaud |
author_facet |
Serge Alain Sadeuh-Mba Marie-Line Joffret Arthur Mazitchi Marie-Claire Endegue-Zanga Richard Njouom Francis Delpeyroux Ionela Gouandjika-Vasilache Maël Bessaud |
author_sort |
Serge Alain Sadeuh-Mba |
title |
Genetic and phenotypic characterization of recently discovered enterovirus D type 111. |
title_short |
Genetic and phenotypic characterization of recently discovered enterovirus D type 111. |
title_full |
Genetic and phenotypic characterization of recently discovered enterovirus D type 111. |
title_fullStr |
Genetic and phenotypic characterization of recently discovered enterovirus D type 111. |
title_full_unstemmed |
Genetic and phenotypic characterization of recently discovered enterovirus D type 111. |
title_sort |
genetic and phenotypic characterization of recently discovered enterovirus d type 111. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007797 https://doaj.org/article/6b4569f9f3fb4b7ca6698445b1bf7595 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 10, p e0007797 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007797 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007797 https://doaj.org/article/6b4569f9f3fb4b7ca6698445b1bf7595 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007797 |
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PLOS Neglected Tropical Diseases |
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13 |
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10 |
container_start_page |
e0007797 |
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