Complete Protection against Pneumonic and Bubonic Plague after a Single Oral Vaccination.
BACKGROUND:No efficient vaccine against plague is currently available. We previously showed that a genetically attenuated Yersinia pseudotuberculosis producing the Yersinia pestis F1 antigen was an efficient live oral vaccine against pneumonic plague. This candidate vaccine however failed to confer...
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ftdoajarticles:oai:doaj.org/article:6afcd0a12b9b40009a01ee006d69872b 2023-05-15T15:15:22+02:00 Complete Protection against Pneumonic and Bubonic Plague after a Single Oral Vaccination. Anne Derbise Yuri Hanada Manal Khalifé Elisabeth Carniel Christian E Demeure 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004162 https://doaj.org/article/6afcd0a12b9b40009a01ee006d69872b EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4608741?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004162 https://doaj.org/article/6afcd0a12b9b40009a01ee006d69872b PLoS Neglected Tropical Diseases, Vol 9, Iss 10, p e0004162 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004162 2022-12-31T13:31:39Z BACKGROUND:No efficient vaccine against plague is currently available. We previously showed that a genetically attenuated Yersinia pseudotuberculosis producing the Yersinia pestis F1 antigen was an efficient live oral vaccine against pneumonic plague. This candidate vaccine however failed to confer full protection against bubonic plague and did not produce F1 stably. METHODOLOGY/PRINCIPAL FINDINGS:The caf operon encoding F1 was inserted into the chromosome of a genetically attenuated Y. pseudotuberculosis, yielding the VTnF1 strain, which stably produced the F1 capsule. Given orally to mice, VTnF1 persisted two weeks in the mouse gut and induced a high humoral response targeting both F1 and other Y. pestis antigens. The strong cellular response elicited was directed mostly against targets other than F1, but also against F1. It involved cells with a Th1-Th17 effector profile, producing IFNγ, IL-17, and IL-10. A single oral dose (108 CFU) of VTnF1 conferred 100% protection against pneumonic plague using a high-dose challenge (3,300 LD50) caused by the fully virulent Y. pestis CO92. Moreover, vaccination protected 100% of mice from bubonic plague caused by a challenge with 100 LD50 Y. pestis and 93% against a high-dose infection (10,000 LD50). Protection involved fast-acting mechanisms controlling Y. pestis spread out of the injection site, and the protection provided was long-lasting, with 93% and 50% of mice surviving bubonic and pneumonic plague respectively, six months after vaccination. Vaccinated mice also survived bubonic and pneumonic plague caused by a high-dose of non-encapsulated (F1-) Y. pestis. SIGNIFICANCE:VTnF1 is an easy-to-produce, genetically stable plague vaccine candidate, providing a highly efficient and long-lasting protection against both bubonic and pneumonic plague caused by wild type or un-encapsulated (F1-negative) Y. pestis. To our knowledge, VTnF1 is the only plague vaccine ever reported that could provide high and durable protection against the two forms of plague after a single oral ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 10 e0004162 |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Anne Derbise Yuri Hanada Manal Khalifé Elisabeth Carniel Christian E Demeure Complete Protection against Pneumonic and Bubonic Plague after a Single Oral Vaccination. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:No efficient vaccine against plague is currently available. We previously showed that a genetically attenuated Yersinia pseudotuberculosis producing the Yersinia pestis F1 antigen was an efficient live oral vaccine against pneumonic plague. This candidate vaccine however failed to confer full protection against bubonic plague and did not produce F1 stably. METHODOLOGY/PRINCIPAL FINDINGS:The caf operon encoding F1 was inserted into the chromosome of a genetically attenuated Y. pseudotuberculosis, yielding the VTnF1 strain, which stably produced the F1 capsule. Given orally to mice, VTnF1 persisted two weeks in the mouse gut and induced a high humoral response targeting both F1 and other Y. pestis antigens. The strong cellular response elicited was directed mostly against targets other than F1, but also against F1. It involved cells with a Th1-Th17 effector profile, producing IFNγ, IL-17, and IL-10. A single oral dose (108 CFU) of VTnF1 conferred 100% protection against pneumonic plague using a high-dose challenge (3,300 LD50) caused by the fully virulent Y. pestis CO92. Moreover, vaccination protected 100% of mice from bubonic plague caused by a challenge with 100 LD50 Y. pestis and 93% against a high-dose infection (10,000 LD50). Protection involved fast-acting mechanisms controlling Y. pestis spread out of the injection site, and the protection provided was long-lasting, with 93% and 50% of mice surviving bubonic and pneumonic plague respectively, six months after vaccination. Vaccinated mice also survived bubonic and pneumonic plague caused by a high-dose of non-encapsulated (F1-) Y. pestis. SIGNIFICANCE:VTnF1 is an easy-to-produce, genetically stable plague vaccine candidate, providing a highly efficient and long-lasting protection against both bubonic and pneumonic plague caused by wild type or un-encapsulated (F1-negative) Y. pestis. To our knowledge, VTnF1 is the only plague vaccine ever reported that could provide high and durable protection against the two forms of plague after a single oral ... |
format |
Article in Journal/Newspaper |
author |
Anne Derbise Yuri Hanada Manal Khalifé Elisabeth Carniel Christian E Demeure |
author_facet |
Anne Derbise Yuri Hanada Manal Khalifé Elisabeth Carniel Christian E Demeure |
author_sort |
Anne Derbise |
title |
Complete Protection against Pneumonic and Bubonic Plague after a Single Oral Vaccination. |
title_short |
Complete Protection against Pneumonic and Bubonic Plague after a Single Oral Vaccination. |
title_full |
Complete Protection against Pneumonic and Bubonic Plague after a Single Oral Vaccination. |
title_fullStr |
Complete Protection against Pneumonic and Bubonic Plague after a Single Oral Vaccination. |
title_full_unstemmed |
Complete Protection against Pneumonic and Bubonic Plague after a Single Oral Vaccination. |
title_sort |
complete protection against pneumonic and bubonic plague after a single oral vaccination. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0004162 https://doaj.org/article/6afcd0a12b9b40009a01ee006d69872b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 10, p e0004162 (2015) |
op_relation |
http://europepmc.org/articles/PMC4608741?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004162 https://doaj.org/article/6afcd0a12b9b40009a01ee006d69872b |
op_doi |
https://doi.org/10.1371/journal.pntd.0004162 |
container_title |
PLOS Neglected Tropical Diseases |
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9 |
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10 |
container_start_page |
e0004162 |
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1766345742092337152 |